Genomic and pathogenic properties of Pseudomonas syringae pv. syringae strains isolated from apricot in East Azerbaijan province, Iran

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dc.contributor.authorVasebi, Yaldaen
dc.contributor.authorKhakvar, Rezaen
dc.contributor.authorFaghihi, Mohammad Mehdien
dc.contributor.authorVinatzer, Boris A.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2019-08-26T14:19:51Zen
dc.date.available2019-08-26T14:19:51Zen
dc.date.issued2019-05en
dc.description.abstractStrains of Pseudomonas syringae pv. syringae (Pss) were isolated from P. armeniaca in different geographic areas in East Azerbaijan province, Iran, and studied for genetic diversity and host preference. Results of morphological, physiological and biochemical tests showed no differences among strains and syrB gene was determined to be present in all strains by PCR using gene-specific primers. Results of antibiotic assays showed that all strains were resistant to ceftriaxone and erythromycin, while tetracycline induced the strongest growth inhibition. In pathogenicity tests, all strains incited progressive necrotic lesions on apricot twigs at inoculated sites. Severity of symptoms was variable on mango leaves, lemon fruits, bean pods and tomato seedlings. To assess genetic diversity among strains, clustering of strains was performed based on partial sequences of the 16S rRNA and the rpoD housekeeping genes and DNA fingerprinting using IS50-PCR analysis. Cluster analysis was performed using the Unweighted Pair Group Method with Arithmetic (UPGMA) method and Jaccard's similarity coefficients. High genetic diversity was detected among strains. All Pss strains were divided into two distinct clusters at the 37% similarity level. In summary, this study indicates that Pss strains isolated from apricot in East Azerbaijan province in Iran, are genetically diverse, do not display host specificity, but are heterogeneous in regard to aggressiveness.en
dc.description.notesThe authors are grateful for financial support from Iran National Science Foundation [Grant number 93005043].en
dc.description.sponsorshipIran National Science Foundation [93005043]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.bcab.2019.101167en
dc.identifier.eissn1878-8181en
dc.identifier.otherUNSP 101167en
dc.identifier.urihttp://hdl.handle.net/10919/93259en
dc.identifier.volume19en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectBacterial cankeren
dc.subjectHost rangeen
dc.subject16S rRNAen
dc.subjectrpoDen
dc.subjectIS50-PCRen
dc.titleGenomic and pathogenic properties of Pseudomonas syringae pv. syringae strains isolated from apricot in East Azerbaijan province, Iranen
dc.title.serialBiocatalysis and Agricultural Biotechnologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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