Guy1, a Y-linked embryonic signal, regulates dosage compensation in Anopheles stephensi by increasing X gene expression

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dc.contributor.authorQi, Yuminen
dc.contributor.authorWu, Yangen
dc.contributor.authorSaunders, Randyen
dc.contributor.authorChen, Xiaoguangen
dc.contributor.authorMao, Chunhongen
dc.contributor.authorBiedler, James K.en
dc.contributor.authorTu, Zhijian Jakeen
dc.contributor.departmentBiochemistryen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2019-10-18T14:41:47Zen
dc.date.available2019-10-18T14:41:47Zen
dc.date.issued2019-03-19en
dc.description.abstractWe previously showed that Guy1, a primary signal expressed from the Y chromosome, is a strong candidate for a male-determining factor that confers female-specific lethality in Anopheles stephensi (Criscione et al., 2016). Here, we present evidence that Guyl increases X gene expression in Guy1-transgenic females from two independent lines, providing a mechanism underlying the Guy1-conferred female lethality. The median level gene expression (MGE) of X-linked genes is significantly higher than autosomal genes in Guy1-transgenic females while there is no significant difference in MGE between X and autosomal genes in wild-type females. Furthermore, Guyl significantly upregulates at least 40% of the 996 genes across the X chromosome in transgenic females. Guy1-conferred female-specific lethality is remarkably stable and completely penetrant. These findings indicate that Guyl regulates dosage compensation in An. stephensi and components of dosage compensation may be explored to develop novel strategies to control mosquito-borne diseases.en
dc.description.notesNational Institutes of Health AI105575 Zhijian Jake Tu; National Institutes of Health AI121284 Zhijian Jake Tu; The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.en
dc.description.sponsorshipNational Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [AI105575, AI121284]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.7754/eLife.43570en
dc.identifier.issn2050-084Xen
dc.identifier.othere43570en
dc.identifier.pmid30888319en
dc.identifier.urihttp://hdl.handle.net/10919/94636en
dc.identifier.volume8en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectsexen
dc.subjectchromosomeen
dc.subjectevolutionen
dc.subjectencodesen
dc.subjectproteinen
dc.titleGuy1, a Y-linked embryonic signal, regulates dosage compensation in Anopheles stephensi by increasing X gene expressionen
dc.title.serialeLifeen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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