Modulation of gap junction-associated Cx43 in neural stem/progenitor cells following traumatic brain injury

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dc.contributor.authorGreer, Kishaen
dc.contributor.authorChen, Jiangen
dc.contributor.authorBrickler, Thomasen
dc.contributor.authorGourdie, Robert G.en
dc.contributor.authorTheus, Michelle H.en
dc.date.accessioned2019-10-03T12:37:19Zen
dc.date.available2019-10-03T12:37:19Zen
dc.date.issued2017-09en
dc.description.abstractRestoration of learning and memory deficits following traumatic brain injury (TBI) is attributed, in part, to enhanced neural stem/progenitor cell (NSPCs) function. Recent findings suggest gap junction (GJ)-associated connexin 43 (Cx43) plays a key role in the cell cycle regulation and function of NSPCs and is modulated following TBI. Here, we demonstrate that Cx43 is up-regulated in the dentate gyrus following TBI and is expressed on vimentin-positive cells in the subgranular zone. To test the role of Cx43 on NSPCs, we exposed primary cultures to the alpha-connexin Carboxyl Terminal (alpha CT1) peptide which selectively modulates GJ-associated Cx43. Treatment with alpha CT1 substantially reduced proliferation and increased caspase 3/7 expression on NSPCs in a dose-dependent manner. alpha CT1 exposure also reduced overall expression of Cx43 and phospho (p)-Serine368. These findings demonstrate that Cx43 positively regulates adult NPSCs; the modulation of which may influence changes in the dentate gyrus following TBI.en
dc.description.notesThe authors would like to thank VT-IMSD Programs for student support (K.M.G.). This work was supported by R01 NS096281 (MHT), diversity supplement NS096281 (MHT, KG), R15 NS081623 (MHT) and VT's Institute of Critical Technology and Science. We recognize the Virginia Maryland College of Veterinary Medicine for student and financial support. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH or any other funding agency.en
dc.description.sponsorshipVT's Institute of Critical Technology and Science; Virginia Maryland College of Veterinary Medicine; [R01 NS096281]; [NS096281]; [R15 NS081623]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.brainresbull.2017.06.016en
dc.identifier.eissn1873-2747en
dc.identifier.issn0361-9230en
dc.identifier.pmid28648814en
dc.identifier.urihttp://hdl.handle.net/10919/94331en
dc.identifier.volume134en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectCx43en
dc.subjectTraumatic brain injuryen
dc.subjectNeural stem progenitor cellen
dc.subjectGap junctionen
dc.subjectApoptosisen
dc.titleModulation of gap junction-associated Cx43 in neural stem/progenitor cells following traumatic brain injuryen
dc.title.serialBrain Research Bulletinen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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Scholarly Works, Biomedical Sciences and Pathobiology
Destination Area: Adaptive Brain and Behavior (ABB)
Scholarly Works, Fralin Biomedical Research Institute at VTC