Deep Sequencing of Pyrethroid-Resistant Bed Bugs Reveals Multiple Mechanisms of Resistance within a Single Population

Simple item page
dc.contributor.authorAdelman, Zach N.en
dc.contributor.authorKilcullen, Kathleen A.en
dc.contributor.authorKoganemaru, Reinaen
dc.contributor.authorAnderson, Michelle A. E.en
dc.contributor.authorAnderson, Troy D.en
dc.contributor.authorMiller, Dini M.en
dc.contributor.departmentEntomologyen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2018-11-06T14:59:23Zen
dc.date.available2018-11-06T14:59:23Zen
dc.date.issued2011-10-19en
dc.description.abstractA frightening resurgence of bed bug infestations has occurred over the last 10 years in the U.S. and current chemical methods have been inadequate for controlling this pest due to widespread insecticide resistance. Little is known about the mechanisms of resistance present in U.S. bed bug populations, making it extremely difficult to develop intelligent strategies for their control. We have identified bed bugs collected in Richmond, VA which exhibit both kdr-type (L925I) and metabolic resistance to pyrethroid insecticides. Using LD50 bioassays, we determined that resistance ratios for Richmond strain bed bugs were ∼5200-fold to the insecticide deltamethrin. To identify metabolic genes potentially involved in the detoxification of pyrethroids, we performed deep-sequencing of the adult bed bug transcriptome, obtaining more than 2.5 million reads on the 454 titanium platform. Following assembly, analysis of newly identified gene transcripts in both Harlan (susceptible) and Richmond (resistant) bed bugs revealed several candidate cytochrome P450 and carboxylesterase genes which were significantly over-expressed in the resistant strain, consistent with the idea of increased metabolic resistance. These data will accelerate efforts to understand the biochemical basis for insecticide resistance in bed bugs, and provide molecular markers to assist in the surveillance of metabolic resistance.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0026228en
dc.identifier.eissn1932-6203en
dc.identifier.issue10en
dc.identifier.othere26228en
dc.identifier.pmid22039447en
dc.identifier.urihttp://hdl.handle.net/10919/85654en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleDeep Sequencing of Pyrethroid-Resistant Bed Bugs Reveals Multiple Mechanisms of Resistance within a Single Populationen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
journal.pone.0026228.PDF
Size:
509.17 KB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Scholarly Works, Entomology