Studies on mechanisms involved in the induction of microsomal benzpyrene hydroxylase following pretreatment with 3,4-benzpyrene anddaflatoxin BO

Simple item page
dc.contributor.authorGurtoo, Hira Lalen
dc.contributor.committeechairCampbell, T. Colinen
dc.contributor.committeememberWebb, Ryland E.en
dc.contributor.committeememberAckerman, C. J.en
dc.contributor.committeememberPlowman, Kent M.en
dc.contributor.committeememberSchmidt, Robert R.en
dc.contributor.departmentBiochemistry and Nutritionen
dc.date.accessioned2014-03-14T21:11:43Zen
dc.date.adate2010-06-02en
dc.date.available2014-03-14T21:11:43Zen
dc.date.issued1968-12-15en
dc.date.rdate2010-06-02en
dc.date.sdate2010-06-02en
dc.description.abstractMicrosomes obtained from different groups or rats, following pretreatment with different dosages or 3,4-benzpyrene (BP), aflatoxin Bl (Bl), ethionine (ET) and/or a combination of these, were used to obtain initial velocity data for BP disappearance. The values for Km, Vmax and K/v for different groups were obtained by the computer analysis of initial velocity data. Vmax and Km values were used to assess the quantitative and qualitative changes of microsomal B:2 hydroxylase, respectively, caused pretreatment. BP pretreatment greatly enhanced the V and also decreased the Km values. The increase in Vmax paralleled the increase in the dosage of BP from BP(lX) to BP(3X). When ET, a protein inhibitor, was given in conjunction with BP it blocked the increase in Vmax but did not block the effect of BP on the Km. The effect of BP on the qualitative properties (Km) of enzyme was observed to be a slow time dependent phenomenon which required the simultaneous presence of both the enzyme and the inducer.en
dc.description.degreePh. D.en
dc.format.extent119 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-06022010-020342en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06022010-020342/en
dc.identifier.urihttp://hdl.handle.net/10919/37933en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1968.G8.pdfen
dc.relation.isformatofOCLC# 20736950en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1968.G8en
dc.subject.lcshEnzymesen
dc.titleStudies on mechanisms involved in the induction of microsomal benzpyrene hydroxylase following pretreatment with 3,4-benzpyrene anddaflatoxin BOen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistry and Nutritionen
thesis.degree.grantorVirginia Polytechnic Instituteen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
LD5655.V856_1968.G8.pdf
Size:
4.79 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Doctoral Dissertations