Human rhinovirus infection causes different DNA methylation changes in nasal epithelial cells from healthy and asthmatic subjects

Simple item page
dc.contributor.authorMcErlean, Peteren
dc.contributor.authorFavoreto, Silvio Jren
dc.contributor.authorCosta, Fabricio F.en
dc.contributor.authorShen, Junqingen
dc.contributor.authorQuraishi, Jihanen
dc.contributor.authorBiyasheva, Asselen
dc.contributor.authorCooper, Jocelyn J.en
dc.contributor.authorScholtens, Denise M.en
dc.contributor.authorVanin, Elio F.en
dc.contributor.authorde Bonaldo, Maria F.en
dc.contributor.authorXie, Hehuang Daviden
dc.contributor.authorSoares, Marcelo B.en
dc.contributor.authorAvila, Pedro C.en
dc.date.accessioned2014-07-03T11:05:07Zen
dc.date.available2014-07-03T11:05:07Zen
dc.date.issued2014-06-19en
dc.date.updated2014-07-03T11:05:09Zen
dc.description.abstractBackground Mechanisms underlying the development of virus-induced asthma exacerbations remain unclear. To investigate if epigenetic mechanisms could be involved in virus-induced asthma exacerbations, we undertook DNA methylation profiling in asthmatic and healthy nasal epithelial cells (NECs) during Human Rhinovirus (HRV) infection in vitro. Methods Global and loci-specific methylation profiles were determined via Alu element and Infinium Human Methylation 450-K microarray, respectively. Principal components analysis identified the genomic loci influenced the most by disease-status and infection. Real-time PCR and pyrosequencing were used to confirm gene expression and DNA methylation, respectively. Results HRV infection significantly increased global DNA methylation in cells from asthmatic subjects only (43.6% to 44.1%, p = 0.04). Microarray analysis revealed 389 differentially methylated loci either based on disease status, or caused by virus infection. There were disease-associated DNA methylation patterns that were not affected by HRV infection as well as HRV-induced DNA methylation changes that were unique to each group. A common methylation locus stood out in response to HRV infection in both groups, where the small nucleolar RNA, H/ACA box 12 (SNORA12) is located. Further analysis indicated that a relationship existed between SNORA12 DNA methylation and gene expression in response to HRV infection. Conclusions We describe for the first time that Human rhinovirus infection causes DNA methylation changes in airway epithelial cells that differ between asthmatic and healthy subjects. These epigenetic differences may possibly explain the mechanism by which respiratory viruses cause asthma exacerbations.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBMC Medical Genomics. 2014 Jun 19;7(1):37en
dc.identifier.doihttps://doi.org/10.1186/1755-8794-7-37en
dc.identifier.urihttp://hdl.handle.net/10919/49303en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderPeter McErlean et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleHuman rhinovirus infection causes different DNA methylation changes in nasal epithelial cells from healthy and asthmatic subjectsen
dc.title.serialBMC Medical Genomicsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 3 of 3
Name:
1755-8794-7-37.xml
Size:
81.79 KB
Format:
Extensible Markup Language
Download
Thumbnail Image
Name:
1755-8794-7-37.pdf
Size:
1.26 MB
Format:
Adobe Portable Document Format
Download
Thumbnail Image
Name:
1755-8794-7-37-S1.tiff
Size:
837 KB
Format:
Tag Image File Format
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.5 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Journal Articles, BioMed Central and SpringerOpen
Scholarly Works, Fralin Life Sciences Institute