Recombination activating gene-2(null) severe combined immunodeficient pigs and mice engraft human induced pluripotent stem cells differently

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dc.contributor.authorChoi, Yun-Jungen
dc.contributor.authorKim, EunSuen
dc.contributor.authorReza, Abu Musa Md Talimuren
dc.contributor.authorHong, Kwonhoen
dc.contributor.authorSong, Hyuken
dc.contributor.authorPark, Chankyuen
dc.contributor.authorCho, Seong-Keunen
dc.contributor.authorLee, Kihoen
dc.contributor.authorPrather, Randall S.en
dc.contributor.authorKim, Jin-Hoien
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2019-09-20T14:37:00Zen
dc.date.available2019-09-20T14:37:00Zen
dc.date.issued2017-09-19en
dc.description.abstractThis study comparatively investigated the transcriptional, physiological, and phenotypic differences of the immune disorder between severe combined immunodeficient (SCID) mouse and pig models. We discovered that the recombination activating gene-2 (Rag-2) SCID mice, but not RAG-2 SCID pigs, showed intense, infrequent, and mild cluster of CD3(+)-, CD4(+)-, and CD8(+) signals respectively, suggesting that distinct species-specific effects exist. Furthermore, the expression of six relevant genes (NFATC1, CD79B, CD2, BLNK, FOXO1, and CD40) was more downregulated than that in the Rag-2 SCID mice, which provides a partial rationale for the death of T/B cells in the lymphoid organs of RAG-2 SCID pigs but not in Rag-2 SCID mice. Further, NK cell maturation-related gene expression was significantly lower in RAG-2 SCID pigs than in Rag-2 SCID mice. Consistently, the RAG-2 SCID pigs, but not Rag-2 SCID mice, developed human induced pluripotent stem cell-derived teratomas that were the same as those of perforin/Rag-2 SCID mice. Therefore, these unexpected findings indicate the superiority of RAG-2 SCID pigs over Rag-2 SCID mice as a suitable model for investigating human diseases.en
dc.description.notesThis work was supported by a grant from the Science Research Center (2015R1A5A1009701) of the National Research Foundation of Korea and Next-Generation BioGreen21 program (No. PJ011328) from Rural Development Administration, Korea.en
dc.description.sponsorshipScience Research Center of the National Research Foundation of Korea [2015R1A5A1009701]; Rural Development Administration, Korea [PJ011328]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.18632/oncotarget.20626en
dc.identifier.eissn1949-2553en
dc.identifier.issue41en
dc.identifier.pmid29050212en
dc.identifier.urihttp://hdl.handle.net/10919/93947en
dc.identifier.volume8en
dc.language.isoenen
dc.rightsCreative Commons Attribution 3.0 Unporteden
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subjectsevere combined immunodeficienten
dc.subjectSCIDen
dc.subjectrecombination activating gene-2en
dc.subjectRAG-2en
dc.subjectTalenen
dc.subjectImmunology and Microbiology Sectionen
dc.subjectImmune responseen
dc.subjectImmunityen
dc.titleRecombination activating gene-2(null) severe combined immunodeficient pigs and mice engraft human induced pluripotent stem cells differentlyen
dc.title.serialOncotargeten
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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