Blood Serum Affects Polysaccharide Production and Surface Protein Expression in S. Aureus

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dc.contributor.authorIslam, Nazrulen
dc.contributor.authorHossain, Khwaja G.en
dc.contributor.authorRoss, Julia M.en
dc.contributor.authorMarten, Mark R.en
dc.contributor.departmentChemical Engineeringen
dc.date.accessioned2021-02-04T22:12:28Zen
dc.date.available2021-02-04T22:12:28Zen
dc.date.issued2017-01en
dc.date.updated2021-02-04T22:12:25Zen
dc.description.abstractS. aureus biofilm serves a major role in pathogenesis. Two of the major components of bacterial biofilm are Polysaccharides intercellular adhesions (PIA) and surface proteins. It is not known how PIA and surface proteins expressions are affected in presence of blood serum. Analyses of surface proteins expressions will provide more effective biomarker discovery that might lead to development of antimicrobial therapeutics to meet the challenges of biofilm-related infections.Secondary cultures of S. aureus Philips, a biofilm-forming bacterium, were generated by inoculating 1 ml of overnight culture into 50 ml of TSB. Bacteria were cultured at several concentrations of blood serum and found that 12.5% supplemented blood serum provide s similar growth curve as normal TSB (100%). One and 2 D SASPAGE were used to separate proteins and the differentially expressed proteins were identified by nano-LC/MS.Polysaccharide intercellular adhesions production was significantly increased due to the addition of blood serum in the media. We also identified two serum proteins, apolipoprotein and globulin (Fc and Fab), that remained attached with the membrane fraction of bacterial proteins.These results have strongly demonstrated that blood serum influences the exopolysaccharide expression in S. aureus.en
dc.description.versionPublished (Publication status)en
dc.format.mediumPrint-Electronicen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.19080/ARGH.2017.02.555589en
dc.identifier.eissn2474-7637en
dc.identifier.issn2474-7637en
dc.identifier.issue3en
dc.identifier.otherPMC5983386 (pmc)en
dc.identifier.pmid29863159 (pubmed)en
dc.identifier.urihttp://hdl.handle.net/10919/102252en
dc.identifier.volume2en
dc.language.isoenen
dc.publisherJuniper Publishersen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectBiofilmen
dc.subjectProteomeen
dc.subjectSerumen
dc.subjectStaphylococcus aureusen
dc.titleBlood Serum Affects Polysaccharide Production and Surface Protein Expression in S. Aureusen
dc.title.serialAdvances in biotechnology & microbiology (Newbury, Calif.)en
dc.typeArticle - Refereeden
dc.type.otherresearch-articleen
dc.type.otherJournal Articleen
pubs.organisational-group/Virginia Tech/Engineeringen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineering/COE Administrationen
pubs.organisational-group/Virginia Techen

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