Sanitizer efficacy towards attached bacteria in a simulated milk pipeline system using pure and mixed cultures

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dc.contributor.authorMosteller, Tracy M.en
dc.contributor.committeechairBishop, J. Russellen
dc.contributor.committeecochairHackney, Cameron Rajen
dc.contributor.committeememberPierson, M. D.en
dc.contributor.committeememberEigel, W. N.en
dc.contributor.committeememberJohnson, Janet M.en
dc.contributor.departmentFood Science and Technologyen
dc.date.accessioned2014-03-14T21:17:17Zen
dc.date.adate2007-08-06en
dc.date.available2014-03-14T21:17:17Zen
dc.date.issued1993en
dc.date.rdate2007-08-06en
dc.date.sdate2007-08-06en
dc.description.abstractThe efficacy of six sanitizers, [chloline (200 ppm), iodophor (2S: ppm), acid anionic (200 ppm), peracetic acid (200 ppm), and fatty acid sanitizer (200 ppm)], was evaluated against bacteria attached to gasket materials. Pseudomonas fluorescens, Yersinia enterocolitica, Bacillus cereus, and Listeria monocvtogenes were capable of significant attachment to both buna-N nlbber and TeflonĀ® gasket surfaces in either pure or mixed cultures. Differences in initial attachment rates were evident in a mixed culture of P. fluorescens, Y. enterocolitica, and Listeria monocytogenes in vitro. Sanitizer effectiveness depended upon the bacterium being enumerated, the type of surface, if the bacterium was attached in pure culture or as part of a mixed culture, and the system of evaluation, (i.e. whether or not sanitizer was used alone or as part of a cleaning system). Peracetic acid was the most effective. Removal of bacteria was more pronounced on the Te'f1onĀ® surface with all sanitizers used. The cleaning system, which consisted of a pre-rinse with warm water, application of the cleaning solution, post-rinse with warm water, and application of the sanitizing solution, allowed microorganisms to remain, when the bacteria were present as a pure culture, but resulted in the complete removal of bacteria in mixed culture.en
dc.description.degreePh. D.en
dc.format.extentxv, 233 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-08062007-094410en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-08062007-094410/en
dc.identifier.urihttp://hdl.handle.net/10919/39061en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1993.M677.pdfen
dc.relation.isformatofOCLC# 29699941en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1993.M677en
dc.subject.lcshBacteria -- Adhesionen
dc.subject.lcshDairy microbiologyen
dc.subject.lcshDisinfection and disinfectantsen
dc.subject.lcshMilk -- Microbiologyen
dc.titleSanitizer efficacy towards attached bacteria in a simulated milk pipeline system using pure and mixed culturesen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineFood Science and Technologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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