High-Frequency Irreversible Electroporation for Treatment of Primary Liver Cancer: A Proof-of-Principle Study in Canine Hepatocellular Carcinoma

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dc.contributor.authorPartridge, Brittanie R.en
dc.contributor.authorO'Brien, Timothy J.en
dc.contributor.authorLorenzo, Melvin F.en
dc.contributor.authorCoutermarsh-Ott, Sherylen
dc.contributor.authorBarry, Sabrina L.en
dc.contributor.authorStadler, Krystina L.en
dc.contributor.authorMuro, Noelleen
dc.contributor.authorMeyerhoeffer, Mitchellen
dc.contributor.authorAllen, Irving C.en
dc.contributor.authorDavalos, Rafael V.en
dc.contributor.authorDervisis, Nikolaos G.en
dc.contributor.departmentSmall Animal Clinical Sciencesen
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.date.accessioned2021-01-07T18:13:52Zen
dc.date.available2021-01-07T18:13:52Zen
dc.date.issued2020-03en
dc.description.abstractPurpose: To determine the safety and feasibility of percutaneous high-frequency irreversible electroporation (HFIRE) for primary liver cancer and evaluate the HFIRE-induced local immune response. Materials and Methods: HFIRE therapy was delivered percutaneously in 3 canine patients with resectable hepatocellular carcinoma (HCC) in the absence of intraoperative paralytic agents or cardiac synchronization. Pre- and post-HFIRE biopsy samples were processed with histopathology and immunohistochemistry for CD3, CD4, CD8, and CD79a. Blood was collected on days 0, 2, and 4 for complete blood count and chemistry. Numeric models were developed to determine the treatment-specific lethal thresholds for malignant canine liver tissue and healthy porcine liver tissue. Results: HFIRE resulted in predictable ablation volumes as assessed by posttreatment CT. No detectable cardiac interference and minimal muscle contraction occurred during HFIRE. No clinically significant adverse events occurred secondary to HFIRE. Microscopically, a well-defined ablation zone surrounded by a reactive zone was evident in the majority of samples. This zone was composed primarily of maturing collagen interspersed with CD3(+)/CD4(-)/CD8(-) lymphocytes in a proinflammatory microenvironment. The average ablation volumes for the canine HCC patients and the healthy porcine tissue were 3.89 cm(3) +/- 0.74 and 1.56 cm(3) +/- 0.16, respectively (P = .03), and the respective average lethal thresholds were 710 V/cm +/- 28.2 and 957 V/cm +/- 24.4 V/cm (P = .0004). Conclusions: HFIRE can safely and effectively be delivered percutaneously, results in a predictable ablation volume, and is associated with lymphocytic tumor infiltration. This is the first step toward the use of HFIRE for treatment of unresectable liver tumors.en
dc.description.notesThe present study was supported by the Veterinary Memorial Fund, Institute for Critical Technology and Applied Science Center for Engineered Health, Pancreatic Cancer Action Network (PanCAN) Grant PanCAN 16-65-IANN, and the Grayton Friedlander Memorial Fund. The authors thank members of the Veterinary Teaching Hospital (VTH) Oncology service (Klahn, Olsen, and Wyne), VTH Radiology service (Stadler), VTH Surgery service (Muro), VTH Anesthesia (Carpenter), Dr. Coy Allen's laboratory (Ringel-Scala and Brock), Dr. John Rossmeisl's laboratory (Arena), and Anne Avery at CSU's Clinical Immunology Lab for all of their contributions to this project.en
dc.description.sponsorshipVeterinary Memorial Fund; Institute for Critical Technology and Applied Science Center for Engineered Health; Pancreatic Cancer Action Network (PanCAN) Grant [PanCAN 16-65-IANN]; Grayton Friedlander Memorial Funden
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.jvir.2019.10.015en
dc.identifier.eissn1535-7732en
dc.identifier.issn1051-0443en
dc.identifier.issue3en
dc.identifier.pmid31956003en
dc.identifier.urihttp://hdl.handle.net/10919/101771en
dc.identifier.volume31en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleHigh-Frequency Irreversible Electroporation for Treatment of Primary Liver Cancer: A Proof-of-Principle Study in Canine Hepatocellular Carcinomaen
dc.title.serialJournal of Vascular and Interventional Radiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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