Characterization of Lilium longiflorum cv. 'Nellie White' Infection with Root-lesion Nematode Pratylenchus penetrans by Bright-field and Transmission Electron Microscopy

Simple item page
dc.contributor.authorVieira, Pauloen
dc.contributor.authorMowery, Josephen
dc.contributor.authorKilcrease, Jamesen
dc.contributor.authorEisenback, Jonathan D.en
dc.contributor.authorKamo, Kathrynen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2020-03-24T19:31:56Zen
dc.date.available2020-03-24T19:31:56Zen
dc.date.issued2017-03en
dc.description.abstractLilium longiflorum cv. Nellie White, commonly known as Easter lily, is an important floral crop with an annual wholesale value of over $26 million in the United States. The root-lesion nematode, Pratylenchus penetrans, is a major pest of lily due to the significant root damage it causes. In this study, we investigated the cytological aspects of this plant-nematode interaction using bright-field and transmission electron microscopy. We took advantage of an in vitro culture method to multiply lilies and follow the nematode infection over time. Phenotypic reactions of roots inoculated with P. penetrans were evaluated from 0 to 60 d after nematode infection. Symptom development progressed from initial randomly distributed discrete necrotic areas to advanced necrosis along entire roots of each inoculated plant. A major feature characterizing this susceptible host response to nematode infection was the formation of necrosis, browning, and tissue death involving both root epidermis and cortical cells. Degradation of consecutive cell walls resulted in loss of cell pressure, lack of cytoplasmic integrity, followed by cell death along the intracellular path of the nematode's migration. Pratylenchus penetrans was never seen in the vascular cylinder as the layer of collapsed endodermal cells presumably blocked the progression of nematodes into this area of the roots. This study presents the first detailed cytological characterization of P. penetrans infection of Easter lily plants.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesThe authors acknowledge the technical assistance of Sarah Shih. This material is based on work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number 11588909.en
dc.description.sponsorshipNational Institute of Food and Agriculture, U.S. Department of AgricultureUnited States Department of Agriculture (USDA) [11588909]en
dc.format.mimetypeapplication/pdfen
dc.identifier.issn0022-300Xen
dc.identifier.issue1en
dc.identifier.pmid28512372en
dc.identifier.urihttp://hdl.handle.net/10919/97455en
dc.identifier.volume49en
dc.language.isoenen
dc.rightsCC0 1.0 Universalen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectcell biologyen
dc.subjectEaster lilyen
dc.subjectelectron microscopyen
dc.subjectLilium longiflorumen
dc.subjectPratylenchus penetransen
dc.subjectroot-lesion nematodeen
dc.titleCharacterization of Lilium longiflorum cv. 'Nellie White' Infection with Root-lesion Nematode Pratylenchus penetrans by Bright-field and Transmission Electron Microscopyen
dc.title.serialJournal of Nematologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
VieiraCharacterization.pdf
Size:
2.37 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Scholarly Works, School of Plant and Environmental Sciences