Multiplexed Analysis of Cage and Cage Free Chicken Egg Fatty Acids Using Stable Isotope Labeling and Mass Spectrometry

dc.contributor.authorTorde, Richard G.en
dc.contributor.authorTherrien, Andrew J.en
dc.contributor.authorShortreed, Michael R.en
dc.contributor.authorSmith, Lloyd M.en
dc.contributor.authorLamos, Shane M.en
dc.date.accessioned2017-09-20T18:19:59Zen
dc.date.available2017-09-20T18:19:59Zen
dc.date.issued2013-12-05en
dc.date.updated2017-09-20T18:20:00Zen
dc.description.abstractBinary stable isotope labeling couple with LC-ESI-MS has been used as a powerful non-targeted approach for the relative quantification of lipids, amino acids, and many other important metabolite classes. A multiplexed approach using three or more isotopic labeling reagents greatly reduces analytical run-time while maintaining excellent sensitivity and reproducibility. Three isotopic cholamine labeling reagents have been developed to take advantage of the pre-ionized character of cholamine, for ESI, and the ease by which stable isotopes can be incorporated into the cholamine structure. These three cholamine labeling reagents have been used to relatively quantify three fatty acid samples simultaneously. The quantification resulted in the observation of 12 fatty acids that had an average absolute error of 0.9% and an average coefficient of variation of 6.1%. Caged<i> versus</i> cage-free isotope labeling experiments showed that cage-free eggs have an increased level of omega-3 fatty acids as compared to caged eggs. This multiplexed fatty acid analysis provides an inexpensive and expedited tool for broad-based lipid profiling that will further aid discoveries in the mechanisms of fatty acid action in cells.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationTorde, R.G.; Therrien, A.J.; Shortreed, M.R.; Smith, L.M.; Lamos, S.M. Multiplexed Analysis of Cage and Cage Free Chicken Egg Fatty Acids Using Stable Isotope Labeling and Mass Spectrometry. Molecules 2013, 18, 14977-14988.en
dc.identifier.doihttps://doi.org/10.3390/molecules181214977en
dc.identifier.urihttp://hdl.handle.net/10919/79198en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectfatty acidsen
dc.subjectcholamineen
dc.subjectpre-ionization chargeen
dc.subjectstable isotope labelingen
dc.subjectmultiplexingen
dc.titleMultiplexed Analysis of Cage and Cage Free Chicken Egg Fatty Acids Using Stable Isotope Labeling and Mass Spectrometryen
dc.title.serialMoleculesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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