A Three-Dimensional in Vitro Tumor Platform for Modeling Therapeutic Irreversible Electroporation

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dc.contributor.authorArena, Christopher B.en
dc.contributor.authorSzot, Cchristopher S.en
dc.contributor.authorGarcia, Paulo A.en
dc.contributor.authorRylander, M. Nicholeen
dc.contributor.authorDavalos, Rafael V.en
dc.contributor.departmentMechanical Engineeringen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessed2014-02-05en
dc.date.accessioned2014-02-26T19:10:03Zen
dc.date.available2014-02-26T19:10:03Zen
dc.date.issued2012-06-01en
dc.description.abstractIrreversible electroporation (IRE) is emerging as a powerful tool for tumor ablation that utilizes pulsed electric fields to destabilize the plasma membrane of cancer cells past the point of recovery. The ablated region is dictated primarily by the electric field distribution in the tissue, which forms the basis of current treatment planning algorithms. To generate data for refinement of these algorithms, there is a need to develop a physiologically accurate and reproducible platform on which to study IRE in vitro. Here, IRE was performed on a 3D in vitro tumor model consisting of cancer cells cultured within dense collagen I hydrogels, which have been shown to acquire phenotypes and respond to therapeutic stimuli in a manner analogous to that observed in in vivo pathological systems. Electrical and thermal fluctuations were monitored during treatment, and this information was incorporated into a numerical model for predicting the electric field distribution in the tumors. When correlated with Live/Dead staining of the tumors, an electric field threshold for cell death (500 V/cm) comparable to values reported in vivo was generated. In addition, submillimeter resolution was observed at the boundary between the treated and untreated regions, which is characteristic of in vivo IRE. Overall, these results illustrate the advantages of using 3D cancer cell culture models to improve IRE-treatment planning and facilitate widespread clinical use of the technology.en
dc.description.sponsorshipNational Science Foundation CBET-1055913, CBET-0955072en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationArena, Christopher B.; Szot, Christopher S.; Garcia, Paulo A.; et al., "A Three-Dimensional In Vitro Tumor Platform for Modeling Therapeutic Irreversible Electroporation," Biophysical Journal 103(9), 2033-2042 (2012); doi: 10.1016/j.bpj.2012.09.017en
dc.identifier.doihttps://doi.org/10.1016/j.bpj.2012.09.017en
dc.identifier.issn0006-3495en
dc.identifier.urihttp://hdl.handle.net/10919/25761en
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006349512010326en
dc.language.isoenen
dc.publisherCell Pressen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectPancreatic-canceren
dc.subjectAblation techniqueen
dc.subjectField distributionen
dc.subjectTissuesen
dc.subjectAblationen
dc.subjectCells ablationen
dc.subjectBreast canceren
dc.subjectMouse modelen
dc.subjectVivoen
dc.subject3Den
dc.subjectLiveren
dc.titleA Three-Dimensional in Vitro Tumor Platform for Modeling Therapeutic Irreversible Electroporationen
dc.title.serialBiophysical Journalen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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Scholarly Works, School of Biomedical Engineering and Sciences
Destination Area: Global Systems Science (GSS)
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