Comparison of diagnostic techniques for detection of Giardia duodenalis in dogs and cats

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dc.contributor.authorSaleh, Meriam N.en
dc.contributor.authorHeptinstall, Jack R.en
dc.contributor.authorJohnson, Eileen M.en
dc.contributor.authorBallweber, Lora R.en
dc.contributor.authorLindsay, David S.en
dc.contributor.authorWerre, Stephen R.en
dc.contributor.authorHerbein, Joel F.en
dc.contributor.authorZajac, Anne M.en
dc.date.accessioned2019-08-05T17:19:28Zen
dc.date.available2019-08-05T17:19:28Zen
dc.date.issued2019-05en
dc.description.abstractBackground An evaluation of currently available in-clinic diagnostic tests for Giardia duodenalis infection of dogs and cats has not been performed. In addition, there is discordance among published diagnostic comparisons. The absence of a true gold standard for detecting Giardia duodenalis also complicates diagnostic evaluations. Objectives To evaluate diagnostic tests commercially available in the United States for detecting Giardia duodenalis in dogs and cats, in comparison to a widely used reference test, the direct immunofluorescent assay (IFA), and also to compare the results of 2 methods of analysis: comparison of diagnostic tests to a reference test (IFA) and Bayesian analysis. Animals Fecal samples from a convenience sample of 388 cats and dogs located in Colorado, Oklahoma, and Virginia. Methods Fecal samples were tested for Giardia duodenalis by zinc sulfate centrifugal fecal flotation and 4 different commercial diagnostic immunoassays. Results were analyzed via Bayesian analysis and by comparison to the IFA as the reference test. Results Sensitivity and specificity by comparison to IFA was >= 82% and >= 90%, respectively, for all diagnostic tests in dogs and cats. When analyzed via Bayesian analysis, sensitivity and specificity were >= 83% and >= 95%, respectively. When ZnSO4 centrifugal fecal flotation results were combined with immunoassay results, there was no longer a significant difference between the sensitivities of the commercial in-clinic immunoassays. Conclusion and Clinical Relevance The Bayesian analysis validates using IFA as the reference test. Differences in commercial in-clinic immunoassay sensitivities can be mitigated when the results are combined with ZnSO4 centrifugal fecal flotation results.en
dc.description.notesThis study was supported by funds from TechLab, Inc, Blacksburg, VA. Portions of this work were presented by Meriam Saleh at the 60th Annual Meeting of the American Association of Veterinary Parasitologists in Boston, MA, July 10-14, 2014, and the 64th Annual Meeting of the American Association of Veterinary Parasitologists in Denver CO July 14-17, 2018.en
dc.description.sponsorshipTechLab, Inc, Blacksburg, VAen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1111/jvim.15491en
dc.identifier.eissn1939-1676en
dc.identifier.issn0891-6640en
dc.identifier.issue3en
dc.identifier.pmid30982235en
dc.identifier.urihttp://hdl.handle.net/10919/92741en
dc.identifier.volume33en
dc.language.isoenen
dc.publisherAmerican College of Veterinary Internal Medicineen
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectcanineen
dc.subjectdirect immunofluorescenten
dc.subjectfecal flotationen
dc.subjectfelineen
dc.subjectimmunoassayen
dc.subjectprotozoaen
dc.titleComparison of diagnostic techniques for detection of Giardia duodenalis in dogs and catsen
dc.title.serialJournal of Veterinary Internal Medicineen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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