Separation and quantitation of the seven forms of vitamin B-6 in plasma and 4-pyridoxic acid in urine of adolescent girls by reverse phase high performance liquid chromatography

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dc.contributor.authorChrisley, Barbara Mcen
dc.contributor.committeechairDriskell, Judy A.en
dc.contributor.committeememberRitchey, Sanford J.en
dc.contributor.committeememberMcNair, Harold M.en
dc.contributor.committeememberJohnson, J.M.en
dc.contributor.committeememberYoung, R.W.en
dc.contributor.departmentHuman Nutrition and Foodsen
dc.date.accessioned2015-06-29T22:07:11Zen
dc.date.available2015-06-29T22:07:11Zen
dc.date.issued1988en
dc.description.abstractThe vitamin B-6 status of seemingly healthy adolescent girls was determined using several accepted and proposed parameters in an effort to establish guidelines for status evaluation. HPLC-derived plasma B-6 vitamer [pyridoxal phosphate (PLP), pyridoxine phosphate (PNP), pyridoxamine phosphate (PMP), pyridoxal (PL), pyridoxine (PN), and pyridoxamine (PM)] and 4-pyridoxic acid (4-PA) concentrations and urinary 4-PA levels of 28 white adolescent females, 12-15 years, having radiomonitored plasma PLP concentrations and coenzyme stimulation of erythrocyte alanine aminotransferase activities indicative of adequate status were determined. Mean daily vitamin B-6 and protein intakes of the subjects were 1.48 mg and 78.3 g, respectively. The ranges for plasma B-6 vitamer and 4-PA concentrations for these subjects which had seemingly adequate vitamin B-6 status were as follows: (nmol/L) PLP, 40.9-122.2; PNP, 0-16.1; PMP, 0-8.1; PL, 0- 15.0; PN, 0-21.9; PM, 0-17.8; and 4-PA, 0-55.7. PLP was the predominant plasma B-6 vitamer as well as being the only vitamer found in plasma of all subjects. Urinary 4-PA concentrations of the girls ranged from 0.11-2.50 pmol/mmol creatinine. The B-6 vitamer values of these white adolescent girls should be of use in the establishment of normal ranges for vitamin B-6 status parameters. HPLC methodologies seem to be advantageous for the rapid and accurate assessment of vitamin B-6 status.en
dc.description.degreePh. D.en
dc.format.extentxi, 119 leavesen
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/10919/53934en
dc.language.isoen_USen
dc.publisherVirginia Polytechnic Institute and State Universityen
dc.relation.isformatofOCLC# 19257978en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1988.C568en
dc.subject.lcshTeenage girls -- Nutritionen
dc.subject.lcshVitamin B6 in human nutritionen
dc.titleSeparation and quantitation of the seven forms of vitamin B-6 in plasma and 4-pyridoxic acid in urine of adolescent girls by reverse phase high performance liquid chromatographyen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineHuman Nutrition and Foodsen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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