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Polymicrobial Biofilm Interaction Between Histophilus somni and Pasteurella multocida

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dc.contributor.authorPetruzzi, Brianaen
dc.contributor.authorDickerman, Allan W.en
dc.contributor.authorLahmers, Kevin K.en
dc.contributor.authorScarratt, William K.en
dc.contributor.authorInzana, Thomas J.en
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.contributor.departmentLarge Animal Clinical Sciencesen
dc.date.accessioned2020-11-24T20:41:02Zen
dc.date.available2020-11-24T20:41:02Zen
dc.date.issued2020-07-10en
dc.description.abstractHistophilus somni and Pasteurella multocida are two of multiple agents responsible for bovine respiratory disease (BRD) in cattle. Following respiratory infection of calves withH. somni,P. multocidamay also be isolated from the lower respiratory tract. BecauseH. somnimay form a biofilm during BRD, we sought to determine ifP. multocidacan co-exist withH. somniin a polymicrobial biofilmin vitroandin vivo. Interactions between the two species in the biofilm were characterized and quantified by fluorescencein situhybridization (FISH). The biofilm matrix of each species was examined using fluorescently tagged lectins (FTL) specific for the exopolysaccharide (EPS) using confocal laser scanning microscopy. Bacterial interactions were determined by auto-aggregation and biofilm morphology.Pasteurella multocidaandH. somniwere evenly distributed in thein vitrobiofilm, and both species contributed to the polymicrobial biofilm matrix. The average biomass and biofilm thickness, and the total carbohydrate and protein content of the biofilm, were greatest when both species were present. Polymicrobial bacterial suspensions auto-aggregated faster than single species suspensions, suggesting physical interactions between the two species. Almost 300P. multocidagenes were significantly differentially regulated when the bacteria were in a polymicrobial biofilm compared to a mono-species biofilm, as determined by RNA-sequencing. As expected, host genes associated with inflammation and immune response were significantly upregulated at the infection site followingH. somnichallenge. EncapsulatedP. multocidaisolates not capable of forming a substantial biofilm enhanced anin vitropolymicrobial biofilm withH. somni, indicating they contributed to the polymicrobial biofilm matrix. Indirect evidence indicated that encapsulatedP. multocidaalso contributed to a polymicrobial biofilmin vivo. Only the EPS ofH. somnicould be detected by FTL staining of bovine tissues following challenge withH. somni. However, both species were isolated and an immune response to the biofilm matrix of both species was greater than the response to planktonic cells, suggesting encapsulatedP. multocidamay take advantage of theH. somnibiofilm to persist in the host during chronic BRD. These results may have important implications for the management and prevention of BRD.en
dc.description.notesThis work was supported by the United States Department of Agriculture-National Institute of Food and Agriculture grant 2013-67015-21314 to TI, and by the Virginia Tech College of Veterinary Medicine.en
dc.description.sponsorshipUnited States Department of Agriculture-National Institute of Food and AgricultureUnited States Department of Agriculture (USDA) [2013-67015-21314]; Virginia Tech College of Veterinary Medicineen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fmicb.2020.01561en
dc.identifier.issn1664-302Xen
dc.identifier.other1561en
dc.identifier.pmid32754136en
dc.identifier.urihttp://hdl.handle.net/10919/100927en
dc.identifier.volume11en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectbiofilmen
dc.subjectpolymicrobialen
dc.subjectbovine respiratory diseaseen
dc.subjectHistophilus somnien
dc.subjectPasteurella multocidaen
dc.titlePolymicrobial Biofilm Interaction Between Histophilus somni and Pasteurella multocidaen
dc.title.serialFrontiers In Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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