The dynamic response of the Arabidopsis root metabolome to auxin and ethylene is not predicted by changes in the transcriptome

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dc.contributor.authorHildreth, Sherry B.en
dc.contributor.authorFoley, Evan E.en
dc.contributor.authorMuday, Gloria K.en
dc.contributor.authorHelm, Richard F.en
dc.contributor.authorWinkel, Brenda S. J.en
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentBiochemistryen
dc.date.accessioned2021-09-29T16:28:08Zen
dc.date.available2021-09-29T16:28:08Zen
dc.date.issued2020-01-20en
dc.date.updated2021-09-29T16:28:05Zen
dc.description.abstractWhile the effects of phytohormones on plant gene expression have been well characterized, comparatively little is known about how hormones influence metabolite profiles. This study examined the effects of elevated auxin and ethylene on the metabolome of Arabidopsis roots using a high-resolution 24 h time course, conducted in parallel to time-matched transcriptomic analyses. Mass spectrometry using orthogonal UPLC separation strategies (reversed phase and HILIC) in both positive and negative ionization modes was used to maximize identification of metabolites with altered levels. The findings show that the root metabolome responds rapidly to hormone stimulus and that compounds belonging to the same class of metabolites exhibit similar changes. The responses were dominated by changes in phenylpropanoid, glucosinolate, and fatty acid metabolism, although the nature and timing of the response was unique for each hormone. These alterations in the metabolome were not directly predicted by the corresponding transcriptome data, suggesting that post-transcriptional events such as changes in enzyme activity and/or transport processes drove the observed changes in the metabolome. These findings underscore the need to better understand the biochemical mechanisms underlying the temporal reconfiguration of plant metabolism, especially in relation to the hormone-metabolome interface and its subsequent physiological and morphological effects.en
dc.description.versionPublished versionen
dc.format.extent15 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 679 (Article number)en
dc.identifier.doihttps://doi.org/10.1038/s41598-019-57161-9en
dc.identifier.eissn2045-2322en
dc.identifier.issn2045-2322en
dc.identifier.issue1en
dc.identifier.orcidHelm, Richard [0000-0001-5317-0925]en
dc.identifier.orcidWinkel, Brenda [0000-0003-3481-024X]en
dc.identifier.other10.1038/s41598-019-57161-9 (PII)en
dc.identifier.pmid31959762en
dc.identifier.urihttp://hdl.handle.net/10919/105106en
dc.identifier.volume10en
dc.language.isoenen
dc.publisherNature Researchen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000562135000002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBIOSYNTHETIC-PATHWAYen
dc.subjectEXPRESSIONen
dc.subjectACCUMULATIONen
dc.subjectTRANSPORTen
dc.subjectLIGNINen
dc.subjectACIDen
dc.subjectPHOSPHOLIPASESen
dc.subjectHOMEOSTASISen
dc.subjectFLAVONOIDSen
dc.subjectMUTANTSen
dc.subject.meshArabidopsisen
dc.subject.meshPlant Rootsen
dc.subject.meshEthylenesen
dc.subject.meshIndoleacetic Acidsen
dc.subject.meshGlucosinolatesen
dc.subject.meshFatty Acidsen
dc.subject.meshPlant Growth Regulatorsen
dc.subject.meshGene Expression Profilingen
dc.subject.meshGene Expressionen
dc.subject.meshGenes, Planten
dc.subject.meshTime Factorsen
dc.subject.meshMass Spectrometryen
dc.subject.meshMetabolomeen
dc.subject.meshTranscriptomeen
dc.titleThe dynamic response of the Arabidopsis root metabolome to auxin and ethylene is not predicted by changes in the transcriptomeen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2019-12-19en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/Biological Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten

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