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Development and characterization of microsatellite loci in the endangered catspaw, Epioblasma obliquata (Bivalvia: Unionidae)

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dc.contributor.authorOrtiz, Katlynen
dc.contributor.authorJones, Jess W.en
dc.contributor.authorHallerman, Eric M.en
dc.date.accessioned2022-02-15T18:01:44Zen
dc.date.available2022-02-15T18:01:44Zen
dc.date.issued2022en
dc.date.updated2022-02-15T18:01:42Zen
dc.description.abstractThe endangered Catspaw, Epioblasma obliquata, is restricted to one known reproducing population in Killbuck Creek, Coshocton County, Ohio. Little is known about the genetic diversity of this small population, and such information is needed to help inform recovery planning. We nonlethally sampled 44 individuals of E. obliquata using buccal swabs, from which we developed and characterized 14 polymorphic microsatellite loci. Significant deviations from Hardy–Weinberg Equilibrium (HWE), showing deficiencies in heterozygotes, were observed at 6 of the 14 loci, and linkage disequilibrium (LD) was observed at 9 (~10%) of 91 possible pairwise comparisons among loci. Allelic diversity ranged from 2 to 15 alleles per locus and averaged 7.6 alleles per locus. Observed heterozygosity per locus ranged from 0.091 to 1.000 and averaged 0.674. Possible explanations for deviations from HWE and LD could be from loci located close together on the same chromosome, segregation of null alleles, family structure within the small population, population bottlenecks, inbreeding, hermaphroditic reproduction, or some combination of these factors. Managers can use these microsatellite markers to assess and monitor genetic diversity in the remaining wild population in Killbuck Creek, prospective broodstock, hatchery-reared progeny, and reintroduced populations founded to promote recovery of the species.en
dc.description.versionAccepted versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.31931/fmbc-d-21-00002en
dc.identifier.orcidHallerman, Eric [0000-0002-5839-858X]en
dc.identifier.urihttp://hdl.handle.net/10919/108367en
dc.identifier.volume25en
dc.language.isoenen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.titleDevelopment and characterization of microsatellite loci in the endangered catspaw, Epioblasma obliquata (Bivalvia: Unionidae)en
dc.title.serialFreshwater Mollusk Biology and Conservationen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Natural Resources & Environmenten
pubs.organisational-group/Virginia Tech/Natural Resources & Environment/Fish and Wildlife Conservationen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Natural Resources & Environment/CNRE T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten

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