Multiple immunity-related genes control susceptibility of Arabidopsis thaliana to the parasitic weed Phelipanche aegyptiaca

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dc.contributor.authorClarke, Christopher R.en
dc.contributor.authorPark, So-Yonen
dc.contributor.authorTuosto, Roberten
dc.contributor.authorJia, Xiaoyanen
dc.contributor.authorYoder, Amandaen
dc.contributor.authorVan Mullekom, Jennifer H.en
dc.contributor.authorWestwood, James H.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.contributor.departmentStatisticsen
dc.date.accessioned2020-08-05T13:49:20Zen
dc.date.available2020-08-05T13:49:20Zen
dc.date.issued2020-06-08en
dc.description.abstractParasitic weeds represent a major threat to agricultural production across the world. Little is known about which host genetic pathways determine compatibility for any host-parasitic plant interaction. We developed a quantitative assay to characterize the growth of the parasitic weed Phelipanche aegyptiaca on 46 mutant lines of the host plant Arabidopsis thaliana to identify host genes that are essential for susceptibility to the parasite. A. thaliana host plants with mutations in genes involved in jasmonic acid biosynthesis/signaling or the negative regulation of plant immunity were less susceptible to P. aegyptiaca parasitization. In contrast, A. thaliana plants with a mutant allele of the putative immunity hub gene Pfd6 were more susceptible to parasitization. Additionally, quantitative PCR revealed that P. aegyptiaca parasitization leads to transcriptional reprograming of several hormone signaling pathways. While most tested A. thaliana lines were fully susceptible to P. aegyptiaca parasitization, this work revealed several host genes essential for full susceptibility or resistance to parasitism. Altering these pathways may be a viable approach for limiting host plant susceptibility to parasitism.en
dc.description.notesThis project was supported by the National Institute of Food and Agricultural through postdoctoral fellowship award 2015-67012-22821 to Christopher Clarke and award 135997 to James Westwood. Additional support was through the US National Science Foundation (IOS-1238057) to James Westwood. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.description.sponsorshipNational Institute of Food and Agricultural [2015-67012-22821, 135997]; US National Science FoundationNational Science Foundation (NSF) [IOS-1238057]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.7717/peerj.9268en
dc.identifier.issn2167-8359en
dc.identifier.othere9268en
dc.identifier.pmid32551199en
dc.identifier.urihttp://hdl.handle.net/10919/99486en
dc.identifier.volume8en
dc.language.isoenen
dc.rightsCreative Commons CC0 1.0 Universal Public Domain Dedicationen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectPhelipanche aegyptiacaen
dc.subjectParasitic plantsen
dc.subjectParasite resistanceen
dc.subjectParasite susceptibilityen
dc.subjectArabidopsis thalianaen
dc.subjectPlant immunityen
dc.titleMultiple immunity-related genes control susceptibility of Arabidopsis thaliana to the parasitic weed Phelipanche aegyptiacaen
dc.title.serialPeerJen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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