Gamogony of Sarcocystis Strixi in Mammalian Cell Cultures

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dc.contributor.authorLindsay, David S.en
dc.contributor.authorVerma, S. K.en
dc.contributor.authorDubey, Jitender P.en
dc.contributor.authorScott, Daviden
dc.contributor.authorvon Dohlen, Alexa Rosypalen
dc.date.accessioned2021-11-18T14:10:54Zen
dc.date.available2021-11-18T14:10:54Zen
dc.date.issued2021-07en
dc.description.abstractWe are interested in the disease ecology of Sarcocystis species that infect birds of prey as definitive and intermediate hosts. The present study was done to test our hypothesis that a laboratory model can be developed for sarcocystis infection in mammals using gamma interferon gene knockout (KO) mice as a source of Sarcocystis strixi bradyzoites and mammalian cell cultures as a source of sporulated S. strixi oocysts. Sporocysts of S. strixi from a naturally infected barred owl (Strix varia) were fed to KO mice to produce sarcocysts, and the enclosed bradyzoites were obtained by acid-pepsin digestion of abdominal and thigh muscles. Bradyzoites, metrocytes, and an unusual spherical stage were seen in digest before the inoculation of host cells. The spherical stages stained dark with Giemsa stain, but no nucleus was observed, and they were seen free and associated with the concave portion of some bradyzoites. Examination of infected cell cultures demonstrated that macrogamonts and microgamonts were present at 24 hr post-inoculation. Since sporulated oocysts were not observed, we had to reject our current hypothesis.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesThis work was supported in part by grant number 1505407 from the National Science Foundation Historically Black Colleges and Universities Undergraduate Program to A.R.V.D. and an Internal Research Competition (IRC) grant from the Virginia-Maryland College of Veterinary Medicine to D.S.L. This research was supported in part by an appointment to the Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA). ORISE is managed by Oak Ridge Associated Universities (ORAU) under DOE contract number DE-SC0014664. All opinions expressed in this paper are the authors' and do not necessarily reflect the policies and views of USDA, ARS, DOE, or ORAU/ORISE. The USDA is an equal opportunity provider and employer.en
dc.description.sponsorshipNational Science Foundation Historically Black Colleges and Universities Undergraduate Program [1505407]; Virginia-Maryland College of Veterinary Medicine; DOEUnited States Department of Energy (DOE) [DE-SC0014664]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1645/20-63en
dc.identifier.eissn1937-2345en
dc.identifier.issn0022-3395en
dc.identifier.issue4en
dc.identifier.pmid34283238en
dc.identifier.urihttp://hdl.handle.net/10919/106675en
dc.identifier.volume107en
dc.language.isoenen
dc.rightsPublic Domain (U.S.)en
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/en
dc.subjectSarcocystis strixien
dc.subjectBarred Owlen
dc.subjectStrix variaen
dc.subjectSarcocystsen
dc.subjectBradyzoitesen
dc.subjectMacrogamontsen
dc.subjectMicrogamontsen
dc.subjectOocystsen
dc.subjectCell Culturesen
dc.titleGamogony of Sarcocystis Strixi in Mammalian Cell Culturesen
dc.title.serialJournal of Parasitologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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