Chemical Proteomic Platform To Identify Citrullinated Proteins

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dc.contributor.authorLewallen, Daniel M.en
dc.contributor.authorBicker, Kevin L.en
dc.contributor.authorSubramanian, Venkataramanen
dc.contributor.authorClancy, Kathleen W.en
dc.contributor.authorSlade, Daniel J.en
dc.contributor.authorMartell, Julianneen
dc.contributor.authorDreyton, Christina J.en
dc.contributor.authorSokolove, Jeremyen
dc.contributor.authorWeerapana, Eranthieen
dc.contributor.authorThompson, Paul R.en
dc.contributor.departmentBiochemistryen
dc.contributor.departmentCenter for Drug Discoveryen
dc.date.accessioned2016-11-09T03:06:02Zen
dc.date.available2016-11-09T03:06:02Zen
dc.date.issued2015-11-20en
dc.description.abstractAnti-citrullinated protein antibodies (ACPAs) are a hallmark of rheumatoid arthritis (RA) and are routinely used for disease diagnosis. Protein citrullination is also increased in cancer and other autoimmune disorders, suggesting that citrullinated proteins may serve as biomarkers for diseases beyond RA. To identify these citrullinated proteins, we developed biotin-conjugated phenylglyoxal (biotin-PG). Using this probe and our platform technology, we identified >50 intracellular citrullinated proteins. More than 20 of these are involved in RNA splicing, suggesting, for the first time, that citrullination modulates RNA biology. Overall, this chemical proteomic platform will play a key role in furthering ourunderstanding of protein citrullination in rheumatoid arthritis and potentially a wider spectrum of inflammatory diseases.en
dc.description.versionPublished versionen
dc.format.extent2520 - 2528 page(s)en
dc.identifier.doihttps://doi.org/10.1021/acschembio.5b00438en
dc.identifier.eissn1554-8937en
dc.identifier.issn1554-8929en
dc.identifier.issue11en
dc.identifier.urihttp://hdl.handle.net/10919/73406en
dc.identifier.volume10en
dc.language.isoenen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.titleChemical Proteomic Platform To Identify Citrullinated Proteinsen
dc.title.serialACS Chemical Biologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

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