Packed capillary columns for liquid chromatography

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dc.contributor.authorWilson, William Henryen
dc.contributor.committeechairMcNair, H.M.en
dc.contributor.committeememberTaylor, Larry T.en
dc.contributor.committeememberAnderson, M.en
dc.contributor.committeememberWightman, James P.en
dc.contributor.committeememberGibson, Harry W.en
dc.contributor.departmentChemistryen
dc.date.accessioned2014-03-14T21:10:52Zen
dc.date.adate2006-05-11en
dc.date.available2014-03-14T21:10:52Zen
dc.date.issued1990en
dc.date.rdate2006-05-11en
dc.date.sdate2006-05-11en
dc.description.abstractThe advantages and disadvantages of packed capillary columns for high performance liquid chromatography are examined. Historically, the advantages are smaller sample and phase consumption, enhancement in sensitivity, easy column synthesis, higher obtainable efficiency, and easier interfacing to other techniques. These points are explored through experiments in microbore ion chromatography, packed fused silica columns, and capillary zone electrophoresis. These studies also address the disadvantages of microscale HPLC which are stringent instrument design, brittle or weak columns, poor column stability, and the lack of commercial instrumentation. The results of these investigations indicate the following. First, the purported sensitivity enhancement is really attributable to solute focusing and not to column miniaturization. Second, column synthesis is still a difficult procedure that requires experience. Third, higher efficiencies are realized, especially when the column diameter to particle diameter ratio is optimized. Fourth, interfacing to other techniques is simplified because of the lower volumetric flow rates. Finally, the only real disadvantages are stringent instrument design and brittle columns in some instances. This thesis offers means for circumventing these difficulties.en
dc.description.degreePh. D.en
dc.format.extentx, 149 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-05112006-154753en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05112006-154753/en
dc.identifier.urihttp://hdl.handle.net/10919/37746en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1990.W567.pdfen
dc.relation.isformatofOCLC# 23716329en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1990.W567en
dc.subject.lcshLiquid chromatography -- Researchen
dc.titlePacked capillary columns for liquid chromatographyen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineChemistryen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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