Neck and capsid architecture of the robust Agrobacterium phage Milano

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dc.contributor.authorSonani, Ravi R.en
dc.contributor.authorEsteves, Nathaniel C.en
dc.contributor.authorHorton, Abigail A.en
dc.contributor.authorKelly, Rebecca J.en
dc.contributor.authorSebastian, Amanda L.en
dc.contributor.authorWang, Fengbinen
dc.contributor.authorKreutzberger, Mark A. B.en
dc.contributor.authorLeiman, Petr G.en
dc.contributor.authorScharf, Birgit E.en
dc.contributor.authorEgelman, Edward H.en
dc.date.accessioned2024-02-01T14:09:30Zen
dc.date.available2024-02-01T14:09:30Zen
dc.date.issued2023-09-08en
dc.description.abstractLarge gaps exist in our understanding of how bacteriophages, the most abundant biological entities on Earth, assemble and function. The structure of the “neck” region, where the DNA-filled capsid is connected to the host-recognizing tail remains poorly understood. We describe cryo-EM structures of the neck, the neck-capsid and neck-tail junctions, and capsid of the Agrobacterium phage Milano. The Milano neck 1 protein connects the 12-fold symmetrical neck to a 5-fold vertex of the icosahedral capsid. Comparison of Milano neck 1 homologs leads to four proposed classes, likely evolved from the simplest one in siphophages to more complex ones in myo- and podophages. Milano neck is surrounded by the atypical collar, which covalently crosslinks the tail sheath to neck 1. The Milano capsid is decorated with three types of proteins, a minor capsid protein (mCP) and two linking proteins crosslinking the mCP to the major capsid protein. The extensive network of disulfide bonds within and between neck, collar, capsid and tail provides an exceptional structural stability to Milano.en
dc.description.versionPublished versionen
dc.format.extent12 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 921 (Article number)en
dc.identifier.doihttps://doi.org/10.1038/s42003-023-05292-1en
dc.identifier.eissn2399-3642en
dc.identifier.issn2399-3642en
dc.identifier.issue1en
dc.identifier.orcidScharf, Birgit [0000-0001-6271-8972]en
dc.identifier.other10.1038/s42003-023-05292-1 (PII)en
dc.identifier.pmid37684529en
dc.identifier.urihttps://hdl.handle.net/10919/117780en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherNature Portfolioen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/37684529en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriophagesen
dc.subject.meshDendritic Spinesen
dc.subject.meshBacteriophagesen
dc.subject.meshCapsiden
dc.subject.meshCapsid Proteinsen
dc.subject.meshAgrobacteriumen
dc.titleNeck and capsid architecture of the robust <i>Agrobacterium</i> phage Milanoen
dc.title.serialCommunications Biologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2023-08-28en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/Biological Sciencesen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen

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