Skeletal muscle autophagy and mitophagy in endurance-trained runners before and after a high-fat meal

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dc.contributor.authorTarpey, Michael D.en
dc.contributor.authorDavy, Kevin P.en
dc.contributor.authorMcMillan, Ryan P.en
dc.contributor.authorBowser, Suzanne M.en
dc.contributor.authorHalliday, Tanya M.en
dc.contributor.authorBoutagy, Nabil E.en
dc.contributor.authorDavy, Brenda M.en
dc.contributor.authorFrisard, Madlyn I.en
dc.contributor.authorHulver, Matthew W.en
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.date.accessioned2017-12-06T19:12:56Zen
dc.date.available2017-12-06T19:12:56Zen
dc.date.issued2017-10-24en
dc.description.abstractObjective: We tested the hypothesis that skeletal muscle of endurance-trained male runners would exhibit elevated autophagy and mitophagy markers, which would be associated with greater metabolic flexibility following a high-fat meal (HFM). Methods: Muscle biopsies were collected to determine differences in autophagy and mitophagy protein markers and metabolic flexibility under fasting conditions and 4 h following a HFM between endurance-trained male runners (n =10) and sedentary, non-obese controls (n = 9). Results: Maximal oxygen consumption (ml・kg・min⁻¹) was approximately 50% higher (p < 0.05) in endurance-trained runners compared with sedentary controls (65.8 ±2.3 and 43.1 ±3.4, respectively). Autophagy markers were similar between groups. Mitophagy and mitochondrial dynamics protein markers were significantly higher in skeletal muscle of endurance-trained runners compared with sedentary controls in the fasted state, although unaffected by the HFM. Skeletal muscle metabolic flexibility was similar between groups when fasted (p > 0.05), but increased in response to the HFM in endurance-trained athletes only (p < 0.005). Key mitophagy markers, phospho-Pink1Thr257 and phospho- ParkinS⁶⁵(r = 0.64, p < 0.005), and phospo-ParkinSer⁶⁵ and phospho-Drp1Ser⁶¹⁶ (r = 0.70, p < 0.05) were correlated only within the endurance-trained group. Autophagy and mitophagy markers were not correlated with metabolic flexibility. Conclusion: In summary, mitophagy may be enhanced in endurance-trained runners based on elevated markers of mitophagy and mitochondrial dynamics. The HFM did not alter autophagy or mitophagy in either group. The absence of a relationship between mitophagy markers and metabolic flexibility suggests that mitophagy is not a key determinant of metabolic flexibility in a healthy population, but further investigation is warranted.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.molmet.2017.10.006en
dc.identifier.urihttp://hdl.handle.net/10919/81055en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherElsevieren
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectMetabolic flexibilityen
dc.subjectAutophagyen
dc.subjectMitophagyen
dc.subjectEndurance trainingen
dc.subjectSkeletal muscleen
dc.titleSkeletal muscle autophagy and mitophagy in endurance-trained runners before and after a high-fat mealen
dc.title.serialMolecular Metabolismen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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