Interaction of Phytophthora sojae Effector Avr1b With E3 Ubiquitin Ligase GmPUB1 Is Required for Recognition by Soybeans Carrying Phytophthora Resistance Rps1-b and Rps1-k Genes

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dc.contributor.authorLi, Shanen
dc.contributor.authorHanlon, Reginaen
dc.contributor.authorWise, Huaen
dc.contributor.authorPal, Narinderen
dc.contributor.authorBrar, Hargeeten
dc.contributor.authorLiao, Chunyuen
dc.contributor.authorGao, Hongyuen
dc.contributor.authorPerez, Elien
dc.contributor.authorZhou, Lecongen
dc.contributor.authorTyler, Brett M.en
dc.contributor.authorBhattacharyya, Madan K.en
dc.date.accessioned2021-11-08T19:34:57Zen
dc.date.available2021-11-08T19:34:57Zen
dc.date.issued2021-10-06en
dc.description.abstractPhytophthora sojae is an oomycete that causes stem and root rot disease in soybean. P. sojae delivers many RxLR effector proteins, including Avr1b, into host cells to promote infection. We show here that Avr1b interacts with the soybean U-box protein, GmPUB1-1, in yeast two-hybrid, pull down, and bimolecular fluorescence complementation (BIFC) assays. GmPUB1-1, and a homeologous copy GmPUB1-2, are induced by infection and encode 403 amino acid proteins with U-Box domains at their N-termini. Non-synonymous mutations in the Avr1b C-terminus that abolish suppression of cell death also abolished the interaction of Avr1b with GmPUB1-1, while deletion of the GmPUB1-1 C-terminus, but not the U box, abolished the interaction. BIFC experiments suggested that the GmPUB1-1-Avr1b complex is targeted to the nucleus. In vitro ubiquitination assays demonstrated that GmPUB1-1 possesses E3 ligase activity. Silencing of the GmPUB1 genes in soybean cotyledons resulted in loss of recognition of Avr1b by gene products encoded by Rps1-b and Rps1-k. The recognition of Avr1k (which did not interact with GmPUB1-1) by Rps1-k plants was not, however, affected following GmPUB1-1 silencing. Furthermore, over-expression of GmPUB1-1 in particle bombardment experiments triggered cell death suggesting that GmPUB1 may be a positive regulator of effector-triggered immunity. In a yeast two-hybrid system, GmPUB1-1 also interacted with a number of other RxLR effectors including Avr1d, while Avr1b and Avr1d interacted with a number of other infection-induced GmPUB proteins, suggesting that the pathogen uses a multiplex of interactions of RxLR effectors with GmPUB proteins to modulate host immunity.</p>en
dc.description.notesThis work was supported in part by grants to MB from the Iowa Soybean Association and Agronomy Department, Iowa State University, in part by grants to BT from the National Science Foundation (IOS #0744875), from the Agriculture and Food Research Initiative of the USDA National Institute of Food and Agriculture (#2007-35319-18100 and #2010-65110-20764), and by support from Oregon State University.en
dc.description.sponsorshipIowa Soybean Association; National Science Foundation (IOS)National Science Foundation (NSF) [0744875]; Agriculture and Food Research Initiative of the USDA National Institute of Food and Agriculture [2007-35319-18100, 2010-65110-20764]; Oregon State Universityen
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fpls.2021.725571en
dc.identifier.issn1664-462Xen
dc.identifier.other725571en
dc.identifier.pmid34691104en
dc.identifier.urihttp://hdl.handle.net/10919/106551en
dc.identifier.volume12en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectsoybean U-box proteinen
dc.subjectE3 ubiquitin-ligaseen
dc.subjectPhytophthora sojaeen
dc.subjectoomyceteen
dc.subjectRXLR effectoren
dc.subjectplant immunityen
dc.titleInteraction of Phytophthora sojae Effector Avr1b With E3 Ubiquitin Ligase GmPUB1 Is Required for Recognition by Soybeans Carrying Phytophthora Resistance Rps1-b and Rps1-k Genesen
dc.title.serialFrontiers in Plant Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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