Tissue-, age- and dose-dependent changes in avian beta-defensin and LEAP2 mRNA abundance in the intestines of Salmonella Typhimurium challenged broilers

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dc.contributor.authorGarcia, Javier S.en
dc.contributor.authorByrd, J. Allenen
dc.contributor.authorWong, Eric A.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2021-03-31T12:23:43Zen
dc.date.available2021-03-31T12:23:43Zen
dc.date.issued2020-03-18en
dc.description.abstractSalmonella is a pathogen normally found in the gastrointestinal tract of poultry. The objective of this study was to determine changes in avian beta-defensin (AvBD) and liver-enriched antimicrobial peptide 2 (LEAP2) mRNA following Salmonella challenge. Day of hatch chicks were challenged with 10(6), 10(7) or 10(8) colony-forming units (cfu) of Salmonella typhimurium. There were dose-, tissue- and age-specific changes in AvBD and LEAP2 mRNA. At 1-day post-infection (dpi) there was a transient upregulation of AvBD1, 8, 10 and 12 mRNA in the 10(8) cfu group. At 5 dpi, all seven AvBD mRNA were downregulated in the ileum, while only AvBD1, 6, 10 and 11 mRNA were downregulated in the jejunum and AvBD6, 8, 10, 12 and 13 were downregulated in the cecum. At 7 dpi, there was downregulation of all seven AvBD mRNA in the duodenum and downregulation of selected AvBD in the jejunum, ileum and cecum. LEAP2 mRNA was downregulated at all doses of Salmonella in the cecum at 1 dpi and in the ileum at 5 dpi. In summary, Salmonella infection caused an initial upregulation followed by a downregulation of AvBD mRNA.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesFunding for this work was provided in part by the Virginia Agricultural Council, the Virginia Agricultural Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agriculture. JG was supported in part by a fellowship from the College of Agriculture and Life Sciences George Washington Carver and Graduate Teaching Scholar programs at Virginia Tech.en
dc.description.sponsorshipVirginia Agricultural Council; Virginia Agricultural Experiment Station; Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agriculture; College of Agriculture and Life Sciences George Washington Carver and Graduate Teaching Scholar programs at Virginia Techen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1080/10495398.2020.1738449en
dc.identifier.eissn1532-2378en
dc.identifier.issn1049-5398en
dc.identifier.pmid32186462en
dc.identifier.urihttp://hdl.handle.net/10919/102903en
dc.language.isoenen
dc.rightsPublic Domainen
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/en
dc.subjectSalmonella typhimuriumen
dc.subjectAvian beta-defensinen
dc.subjectLEAP2en
dc.titleTissue-, age- and dose-dependent changes in avian beta-defensin and LEAP2 mRNA abundance in the intestines of Salmonella Typhimurium challenged broilersen
dc.title.serialAnimal Biotechnologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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