Fecal egg counts for gastrointestinal nematodes are associated with a polymorphism in the MHC-DRB1 gene in the Iranian Ghezel sheep breed

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dc.contributor.authorValilou, Rahman Hajializadehen
dc.contributor.authorRafat, Seyed A.en
dc.contributor.authorNotter, David R.en
dc.contributor.authorShojda, Diehlen
dc.contributor.authorMoghaddam, Gholamalien
dc.contributor.authorNematollahi, Ahmaden
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2019-11-04T18:34:08Zen
dc.date.available2019-11-04T18:34:08Zen
dc.date.issued2015-03-24en
dc.description.abstractGenetic variation among sheep breeds in resistance to gastrointestinal nematodes (GIN) has been demonstrated in several production environments. Relationships between the ovine major histocompatibility complex and resistance to GIN have been studied, but few studies have systematically examined this issue in less-developed and semi-arid regions. The aim of the current study was to explore associations between fecal worm egg counts (FEC) for several GIN and polymorphisms in the DRB1 gene. One hundred male lambs were selected at 4-6 months of age from weaned animals in five flocks (n = 20 per flock). Body weights were determined, FAMACHA scores based on color of the ocular mucous membranes were assigned as an indicator of anemia, and blood and fecal samples were collected twice to evaluate FEC and blood packed cell volume (PCV) and for DNA isolation. A repeated-measures analysis of variance was used to test effects of genotype on FEC. The model included fixed effects of flock, genotype, time of measurement (1 or 2), and flock x time and genoype x time interactions, and a random (repeated) effect of lamb. Two genotypes (A1A1 and A1A2) were observed following digestion of Region 1 of Ovar-DRB1 with Pstl. Genotypic frequencies were 0.73 for Al Al and 0.27 for A1A2. FEC differed between Ovar_DRB1 genotypes A1A1 and A1A2 for Marshallagia marshalli, Strongyle, and total nematode FEC. Observed FEC were 30 41% lower for genotype A1A1. Differences among genotypes were consistent across measurement times, with no effect of genotype x measurement time interaction for any parasite class (P >= 0.34). A significant association was observed between FAMACHA scores and lamb PCV, and the residual correlation between these two variables was - 0.51 (P < 0.001). FAMACHA scores can thus be used to detect differences among lambs in PCV, and polymorphic markers of Ovar-DRB1 have potential value as an indicator of parasite resistance in applied animal breeding programs on sheep farms in this region.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fgene.2015.00105en
dc.identifier.issn1664-8021en
dc.identifier.otherUNSP 105en
dc.identifier.pmid25852746en
dc.identifier.urihttp://hdl.handle.net/10919/95245en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectnematodesen
dc.subjectgenetic resistanceen
dc.subjectFAMACHAen
dc.subjectMHC-DRB1en
dc.subjectPCR-RFLPen
dc.subjectsheepen
dc.titleFecal egg counts for gastrointestinal nematodes are associated with a polymorphism in the MHC-DRB1 gene in the Iranian Ghezel sheep breeden
dc.title.serialFrontiers in Geneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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