Proteomics Reveals Distinct Changes Associated with Increased Gamma Radiation Resistance in the Black Yeast Exophiala dermatitidis

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dc.contributor.authorSchultzhaus, Zachary S.en
dc.contributor.authorSchultzhaus, Janna N.en
dc.contributor.authorRomsdahl, Jillianen
dc.contributor.authorChen, Amyen
dc.contributor.authorHervey, W. Judson, IVen
dc.contributor.authorLeary, Dagmar H.en
dc.contributor.authorWang, Zhengen
dc.contributor.departmentSchool of Medicineen
dc.date.accessioned2020-09-28T12:40:53Zen
dc.date.available2020-09-28T12:40:53Zen
dc.date.issued2020-09-25en
dc.date.updated2020-09-25T13:31:00Zen
dc.description.abstractThe yeast <i>Exophiala dermatitidis</i> exhibits high resistance to &gamma;-radiation in comparison to many other fungi. Several aspects of this phenotype have been characterized, including its dependence on homologous recombination for the repair of radiation-induced DNA damage, and the transcriptomic response invoked by acute &gamma;-radiation exposure in this organism. However, these findings have yet to identify unique &gamma;-radiation exposure survival strategies&mdash;many genes that are induced by &gamma;-radiation exposure do not appear to be important for recovery, and the homologous recombination machinery of this organism is not unique compared to more sensitive species. To identify features associated with &gamma;-radiation resistance, here we characterized the proteomes of two <i>E. dermatitidis</i> strains&mdash;the wild type and a hyper-resistant strain developed through adaptive laboratory evolution&mdash;before and after &gamma;-radiation exposure. The results demonstrate that protein intensities do not change substantially in response to this stress. Rather, the increased resistance exhibited by the evolved strain may be due in part to increased basal levels of single-stranded binding proteins and a large increase in ribosomal content, possibly allowing for a more robust, induced response during recovery. This experiment provides evidence enabling us to focus on DNA replication, protein production, and ribosome levels for further studies into the mechanism of &gamma;-radiation resistance in <i>E. dermatitidis</i> and other fungi.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSchultzhaus, Z.S.; Schultzhaus, J.N.; Romsdahl, J.; Chen, A.; Hervey IV, W.J.; Leary, D.H.; Wang, Z. Proteomics Reveals Distinct Changes Associated with Increased Gamma Radiation Resistance in the Black Yeast Exophiala dermatitidis. Genes 2020, 11, 1128.en
dc.identifier.doihttps://doi.org/10.3390/genes11101128en
dc.identifier.urihttp://hdl.handle.net/10919/100082en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectblack yeasten
dc.subjectmelaninen
dc.subjectproteomicsen
dc.subjectradiobiologyen
dc.subjecttranslationen
dc.titleProteomics Reveals Distinct Changes Associated with Increased Gamma Radiation Resistance in the Black Yeast Exophiala dermatitidisen
dc.title.serialGenesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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