Temporal response of ectopic activity in guinea pig ventricular myocardium in response to isoproterenol and acetylcholine

Simple item page
dc.contributor.authorGreer-Short, Amara D.en
dc.contributor.authorPoelzing, Stevenen
dc.contributor.departmentFralin Biomedical Research Instituteen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2017-02-03T15:09:05Zen
dc.date.available2017-02-03T15:09:05Zen
dc.date.issued2015-10-20en
dc.description.abstractBoth beta adrenergic and musoarinio receptor stimulation independently potentiate arrhy-thmogenesis. However, the effect of simultaneous stimulation on arrhy-thmogenesis is not well known. The purpose of this study was to determine the temporal response of arrhythmia risk to individual and combined autonomic agonists. Guinea pig hearts were excised and Langendorff-perfused. The beta adrenergic receptor and musoarinio receptor agonists were isoproterenol (ISO, 0.6 mu M) and acetylcholine (ACh, 10 mu M), respectively. All measurements with agonists occurred over 21 min. ISO induced ectopic activity for the first 8 min. ISO also transiently shortened and then prolonged R-R interval over a similar time course. ACh added after ISO transiently induced ectopic activity for 12 min, while R-R interval invariantly prolonged. ACh alone produced few ectopic beats, while invariantly prolonging R-R interval. In contrast to ISO alone, ISO following ACh significantly increased ectopic activity and shortened R-R interval for the duration of the experiment. Animals aged 17-19 months exhibited sustained arrhythmogenesis while those aged 11-14 did not. When ACh was removed in older hearts while ISO perfused, a transient increase in ectopic activity and decreased R-R interval was observed, similar to ISO alone. These data suggest that pre-treating with and maintaining ACh perfusion can sustain ISO sensitivity, in contrast to ISO perfusion alone.en
dc.description.versionPublished versionen
dc.format.extent? - ? (9) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fphys.2015.00278en
dc.identifier.issn1664-042Xen
dc.identifier.urihttp://hdl.handle.net/10919/74919en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherFrontiersen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000366736900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectPhysiologyen
dc.subjectectopicen
dc.subjectbeta adrenergicen
dc.subjectmuscarinicen
dc.subjectautonomicen
dc.subjectarrhythmiaen
dc.subjectBETA-ADRENERGIC RECEPTORSen
dc.subjectG-PROTEINen
dc.subjectDELAYED AFTERDEPOLARIZATIONSen
dc.subjectACCENTUATED ANTAGONISMen
dc.subjectRYANODINE RECEPTORen
dc.subjectCARDIAC MYOCYTESen
dc.subjectNERVOUS-SYSTEMen
dc.subjectCA2+ CURRENTen
dc.subjectHEARTen
dc.subjectCELLSen
dc.titleTemporal response of ectopic activity in guinea pig ventricular myocardium in response to isoproterenol and acetylcholineen
dc.title.serialFrontiers in Physiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Virginia Tech Carilion Research Instituteen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Temporal response of ectopic activity in guinea pig ventricular myocardium in response to isoproterenol and acetylcholine.pdf
Size:
1.72 MB
Format:
Adobe Portable Document Format
Description:
Publisher's Version
Download
License bundle
Now showing 1 - 1 of 1
Name:
VTUL_Distribution_License_2016_05_09.pdf
Size:
18.09 KB
Format:
Adobe Portable Document Format
Description:
Download

Collections

All Faculty Deposits
Scholarly Works, Fralin Biomedical Research Institute at VTC
Scholarly Works, School of Biomedical Engineering and Sciences