Resistance to Gray Leaf Spot of Maize: Genetic Architecture and Mechanisms Elucidated through Nested Association Mapping and Near-Isogenic Line Analysis

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dc.contributor.authorBenson, Jacqueline M.en
dc.contributor.authorPoland, Jesse A.en
dc.contributor.authorBenson, Brent M.en
dc.contributor.authorStromberg, Erik L.en
dc.contributor.authorNelson, Rebecca J.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2019-10-22T17:51:29Zen
dc.date.available2019-10-22T17:51:29Zen
dc.date.issued2015-03en
dc.description.abstractGray leaf spot (GLS), caused by Cercospora zeae-maydis and Cercospora zeina, is one of the most important diseases of maize worldwide. The pathogen has a necrotrophic lifestyle and no major genes are known for GLS. Quantitative resistance, although poorly understood, is important for GLS management. We used genetic mapping to refine understanding of the genetic architecture of GLS resistance and to develop hypotheses regarding the mechanisms underlying quantitative disease resistance (QDR) loci. Nested association mapping (NAM) was used to identify 16 quantitative trait loci (QTL) for QDR to GLS, including seven novel QTL, each of which demonstrated allelic series with significant effects above and below the magnitude of the B73 reference allele. Alleles at three QTL, qGLS1.04, qGLS2.09, and qGLS4.05, conferred disease reductions of greater than 10%. Interactions between loci were detected for three pairs of loci, including an interaction between iqGLS4.05 and qGLS7.03. Near-isogenic lines (NILs) were developed to confirm and fine-map three of the 16 QTL, and to develop hypotheses regarding mechanisms of resistance. qGLS1.04 was fine-mapped from an interval of 27.0 Mb to two intervals of 6.5 Mb and 5.2 Mb, consistent with the hypothesis that multiple genes underlie highly significant QTL identified by NAM. qGLS2.09, which was also associated with maturity (days to anthesis) and with resistance to southern leaf blight, was narrowed to a 4-Mb interval. The distance between major leaf veins was strongly associated with resistance to GLS at qGLS4.05. NILs for qGLS1.04 were treated with the C. zeae-maydis toxin cercosporin to test the role of host-specific toxin in QDR. Cercosporin exposure increased expression of a putative flavin-monooxygenase (FMO) gene, a candidate detoxification-related gene underlying qGLS1.04. This integrated approach to confirming QTL and characterizing the potential underlying mechanisms advances the understanding of QDR and will facilitate the development of resistant varieties.en
dc.description.notesThis research was supported (in part) by the Cornell University Agricultural Experiment Station federal formula funds, Project No. NYC-153418 and NYC-153462 received from the National Institute of Food and Agriculture (NIFA) United States Department of Agriculture. Any opinions, findings, conclusions, or recommendations expressed in the publication are those of the authors and do not necessarily reflect the view of the National Institute of Food and Agriculture (NIFA) or the United States Department of Agriculture (USDA). This research was also supported (in part) by the McKnight Foundation (http://www.mcknight.org/) and Cornell University (http://www.cornell.edu). These funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.description.sponsorshipCornell University Agricultural Experiment Station; National Institute of Food and Agriculture (NIFA) United States Department of Agriculture [NYC-153418, NYC-153462]; McKnight Foundation; Cornell Universityen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pgen.1005045en
dc.identifier.issn1553-7404en
dc.identifier.issue3en
dc.identifier.othere1005045en
dc.identifier.pmid25764179en
dc.identifier.urihttp://hdl.handle.net/10919/95023en
dc.identifier.volume11en
dc.language.isoenen
dc.publisherPLoSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleResistance to Gray Leaf Spot of Maize: Genetic Architecture and Mechanisms Elucidated through Nested Association Mapping and Near-Isogenic Line Analysisen
dc.title.serialPLOS Geneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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