Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes

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dc.contributor.authorCoscia, Erin C.en
dc.contributor.authorAbutaleb, Nader S.en
dc.contributor.authorHostetter, Bradleyen
dc.contributor.authorSeleem, Mohamed N.en
dc.contributor.authorBreur, Gert J.en
dc.contributor.authorMcCain, Robyn R.en
dc.contributor.authorCrain, Christa J.en
dc.contributor.authorSlaby, Ondrejen
dc.contributor.authorCapoor, Manu N.en
dc.contributor.authorMcDowell, Andrewen
dc.contributor.authorAhmed, Fahad S.en
dc.contributor.authorVijayanpillai, Vijuen
dc.contributor.authorNarayanan, Sanjeev K.en
dc.contributor.authorCoscia, Michael F.en
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.date.accessioned2021-04-28T14:49:35Zen
dc.date.available2021-04-28T14:49:35Zen
dc.date.issued2021-03en
dc.description.abstractThe anaerobic bacterium Cutibacterium acnes has been increasingly linked to the development of degenerative disc disease (DDD), although causality is yet to be conclusively proven. To better study how this organism could contribute to the aetiology of DDD, improved animal models that are more reflective of human disc anatomy, biology and mechanical properties are required. Against this background, our proof-of concept study aimed to be the first demonstration that C. acnes could be safely administered percutaneously into sheep intervertebral discs (IVDs) for in vivo study. Following our protocol, two sheep were successfully injected with a strain of C. acnes (8.3 x 10(6) CFU/disc) previously recovered from a human degenerative disc. No adverse reactions were noted, and at one-month post inoculation all triplicate infected discs in our first animal grew C. acnes, albeit at a reduced load (5.12 x 10(4) to 6.67 x 10(4) CFU/disc). At six months, no growth was detected in discs from our second animal indicating bacterial clearance. This pilot study has demonstrated the feasibility of safe percutaneous injection of C. acnes into sheep IVDs under fluoroscopic guidance. The design of follow-up sheep studies to investigate the potential of C. acnes to drive pathological changes within infected discs should now be pursued.en
dc.description.notesThis research was funded by a grant from the Indiana Orthopaedic Hospital Research Foundation.en
dc.description.sponsorshipIndiana Orthopaedic Hospital Research Foundationen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3390/vetsci8030048en
dc.identifier.eissn2306-7381en
dc.identifier.issue3en
dc.identifier.other48en
dc.identifier.pmid33809558en
dc.identifier.urihttp://hdl.handle.net/10919/103162en
dc.identifier.volume8en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectCutibacterium acnesen
dc.subjectsheep modelen
dc.subjectspinal intervertebral discsen
dc.subjectpercutaneous injectionsen
dc.subjectbacterial discitisen
dc.titleSheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnesen
dc.title.serialVeterinary Sciencesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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