Genetic diversity of Plasmodium vivax in clinical isolates from Bangladesh

dc.contributor.authorKibria, Mohammad G.en
dc.contributor.authorElahi, Rubayeten
dc.contributor.authorMohon, Abu N.en
dc.contributor.authorKhan, Wasif A.en
dc.contributor.authorHaque, Rashidulen
dc.contributor.authorAlam, Mohammad S.en
dc.contributor.departmentBiochemistryen
dc.date.accessioned2015-07-31T16:41:01Zen
dc.date.available2015-07-31T16:41:01Zen
dc.date.issued2015-07-11en
dc.date.updated2015-07-31T16:41:01Zen
dc.description.abstractBackground Plasmodium vivax is the second most prevalent human malaria parasite in Bangladesh; however, there are no data of its genetic diversity. Several molecular markers are available where Pvcsp, Pvmsp 1 and Pvmsp 3α are most commonly used for P. vivax genotyping studies. The aim of the study was to investigate the population structure of P. vivax in Bangladesh. Methods A total of 102 P. vivax-positive blood samples were collected from different malaria-endemic areas in Bangladesh and subsequently analysed for those three genotyping markers. Nested PCR was performed for diagnosis and genotyping analysis followed by PCR–RFLP to detect genetic diversity using Pvcsp, Pvmsp 1 and Pvmsp 3α markers. Results Analysis of Pvcsp showed that the VK210 repeat type was highly prevalent (64.7%, 66/102) compared to VK247 (35.3%, 36/102), although the prevalence of VK247 was higher than other Southeast Asian countries. Analysis of these three genes revealed a diverse, circulating population of P. vivax where a total of ten, 56 and 35 distinct genotypes were detected for Pvcsp, Pvmsp 1 and Pvmsp 3α, respectively. Conclusion This genotyping observation of P. vivax is the first report from Bangladesh and will provide valuable information for establishing the genotyping methods and circulating genetic variants of these three markers available in Bangladesh.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMalaria Journal. 2015 Jul 11;14(1):267en
dc.identifier.doihttps://doi.org/10.1186/s12936-015-0790-4en
dc.identifier.urihttp://hdl.handle.net/10919/55028en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderKibria et al.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleGenetic diversity of Plasmodium vivax in clinical isolates from Bangladeshen
dc.title.serialMalaria Journalen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
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