Comparison of two DNA extraction methods from larvae, pupae, and adults of Aedes aegypti

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dc.contributor.authorde la Cruz-Ramos, Josue M.en
dc.contributor.authorHernandez-Triana, Luis M.en
dc.contributor.authorGarcia-De la Pena, Cristinaen
dc.contributor.authorGonzalez-Alvarez, Vicente H.en
dc.contributor.authorWeger-Lucarelli, Jamesen
dc.contributor.authorKarmy Siller-Rodriguez, Quetzalyen
dc.contributor.authorSanchez Ramos, Francisco J.en
dc.contributor.authorRodriguez, Americo D.en
dc.contributor.authorOrtega-Morales, Aldo, I.en
dc.date.accessioned2020-02-11T14:19:24Zen
dc.date.available2020-02-11T14:19:24Zen
dc.date.issued2019-10en
dc.description.abstractMosquitoes are the most important arthropods from the point of view of public health, due to the fact that they can transmit a large number of pathogens which can cause diseases to humans and animals. Aedes aegypti (L.) is one of the most important vector species in the world, since it can transmit numerous pathogens such as dengue, Zika, and chikungunya. Therefore, studies involving the molecular aspects of this and other mosquitoes species are currently increasing. In this report, we describe the comparison between two DNA extraction techniques, Chelex and cetyltrimethylammonium bromide (CTAB), for carrying out DNA extraction in larvae, pupae and adult female of Ae. aegypti. The Chelex technique was superior in the amount and purity of DNA as compared to the CTAB technique in the three life stages we tested.en
dc.description.notesLuis M Hernandez-Triana would like to thank the Department for Environment Food and Rural Affairs (DEFRA), Scottish Government and Welsh Government through grants SV3045, and SE4113, and the EU Framework Horizon 2020 Innovation Grant, European Virus Archive (EVAg, grant no. 653316) for funding.en
dc.description.sponsorshipDepartment for Environment Food and Rural Affair (DEFRA), Scottish Government [SV3045, SE4113]; Department for Environment Food and Rural Affair (DEFRA), Welsh Government [SV3045, SE4113]; EUEuropean Union (EU) [653316]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.heliyon.2019.e02660en
dc.identifier.issn2405-8440en
dc.identifier.issue10en
dc.identifier.othere02660en
dc.identifier.pmid31692696en
dc.identifier.urihttp://hdl.handle.net/10919/96796en
dc.identifier.volume5en
dc.language.isoenen
dc.rightsOpen Government License (OGL)en
dc.rights.urihttp://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/en
dc.subjectMicrobiologyen
dc.subjectGeneticsen
dc.subjectBiochemistryen
dc.subjectMolecular biologyen
dc.subjectPublic healthen
dc.subjectAedes aegyptien
dc.subjectDNA extractionen
dc.subjectComparisonen
dc.subjectChelexen
dc.subjectCTABen
dc.titleComparison of two DNA extraction methods from larvae, pupae, and adults of Aedes aegyptien
dc.title.serialHeliyonen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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Scholarly Works, Biomedical Sciences and Pathobiology
Destination Area: Global Systems Science (GSS)