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Miniature fiber-optic multicavity Fabry-Perot interferometric biosensor

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dc.contributor.authorZhang, Yanen
dc.contributor.committeechairWang, Anboen
dc.contributor.committeememberHeflin, James R.en
dc.contributor.committeememberJacobs, Iraen
dc.contributor.committeememberSafaai-Jazi, Ahmaden
dc.contributor.committeememberPickrell, Gary R.en
dc.contributor.departmentElectrical and Computer Engineeringen
dc.date.accessioned2014-03-14T20:20:20Zen
dc.date.adate2005-12-22en
dc.date.available2014-03-14T20:20:20Zen
dc.date.issued2005-12-06en
dc.date.rdate2005-12-22en
dc.date.sdate2005-12-15en
dc.description.abstractFiber-optic Fabry-Perot interferometric (FFPI) sensors have been widely used due to their high sensitivity, ease of fabrication, miniature size, and capability for multiplexing. However, direct measurement of self-assembled thin films, receptor immobilization process or biological reaction is limited in the FFPI technique due to the difficulty of forming Fabry-Perot cavities by the thin film itself. Novel methods are needed to provide an accurate and reliable measurement for monitoring the thin-film growth in the nanometer range and under various conditions. In this work, two types of fiber-optic multicavity Fabry-Perot interferometric (MFPI) sensors with built-in temperature compensation were designed and fabricated for thin-film measurement, with applications in chemical and biological sensing. Both the tubing-based MFPI sensor and microgap MFPI sensor provide simple, yet high performance solutions for thin-film sensing. The temperature dependence of the sensing cavity is compensated by extracting the temperature information from a second multiplexed cavity. This provides the opportunity to examine the thin-film characteristics under different environment temperatures. To demonstrate the potential of this structure for practical applications, immunosensors were fabricated and tested using these structures. Self-assembled polyelectrolytes served as a precursor film for immobilization of antibodies to ensure they retain their biological activity. This not only provides a convenient method for protein immobilization but also presents the possibility of increasing the binding capacity and sensitivity by incorporating multilayers of antibodies into polyelectrolyte layers. The steady-state measurement demonstrated the surface concentration and binding ratio of the immunoreaction. Analysis of the kinetic binding profile provided a fast and effective way to measure antigen concentration. Monitoring the immunoreaction between commercially available immunoglobulin G (IgG) and anti-IgG demonstrated the feasibility of using the MFPI sensing system for immunosensing applications.en
dc.description.degreePh. D.en
dc.identifier.otheretd-12152005-213148en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-12152005-213148/en
dc.identifier.urihttp://hdl.handle.net/10919/30104en
dc.publisherVirginia Techen
dc.relation.haspartdissertation.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectTemperature compensationen
dc.subjectPolyelectrolyte self-assemblyen
dc.subjectFiber optic biosensoren
dc.subjectFabry-Perot interferometryen
dc.titleMiniature fiber-optic multicavity Fabry-Perot interferometric biosensoren
dc.typeDissertationen
thesis.degree.disciplineElectrical and Computer Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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