Metagonimoides oregonensis (heterophyidae: digenea) infection in pleurocerid snails and desmognathus quadramaculatus salamander larvae in southern Appalachian streams

dc.contributor.authorBelden, Lisa K.en
dc.contributor.authorPeterman, William E.en
dc.contributor.authorSmith, Stephen A.en
dc.contributor.authorBrooks, Lauren R.en
dc.contributor.authorBenfield, Ernest F.en
dc.contributor.authorBlack, Wesley P.en
dc.contributor.authorYang, Zhaominen
dc.contributor.authorWojdak, Jeremy M.en
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.coverage.countryUnited Statesen
dc.date.accessed2014-06-16en
dc.date.accessioned2014-06-20T14:13:17Zen
dc.date.available2014-06-20T14:13:17Zen
dc.date.issued2012-08en
dc.description.abstractMetagonimoides oregonensis (Heterophyidae) is a little-known digenetic trematode that uses raccoons and possibly mink as definitive hosts, and stream snails and amphibians as intermediate hosts. Some variation in the life cycle and adult morphology in western and eastern populations has been previously noted. In the southern Appalachians, Pleurocera snails and stream salamanders, e.g., Desmognathus spp., are used as intermediate hosts in the life cycle. We completed a series of studies in this system examining some aspects of larval trematode morphology and first and second intermediate host use. Molecular sequencing of the 28S rDNA of cercariae in our survey placed them clearly within the heterophyid family. However, light and scanning electron microscopy revealed both lateral and dorso-ventral finfolds on the cercariae in our region, whereas original descriptions of M. oregonensis cercariae from the west coast indicate only a dorso-ventral finfold, so further work on the systematics of this group may be warranted. A survey of first intermediate host, Pleurocera proxima, from 7 streams in the region identified only M. oregonensis, virgulate-type cercariae, and cotylomicrocercous-type cercariae in the streams, with M. oregonensis having the highest prevalence, and the only type present that use amphibians as second intermediate hosts. Based on clearing and staining of 6 Desmognathus quadramaculatus salamander larvae, we found that individual salamanders could have over 600 metacercariae, which form between muscle fibers throughout the body. Histological observations suggest that the metacercariae do not cause excessive tissue damage or inflammation, and likely persist through metamorphosis, thereby transmitting potentially large numbers of worms to definitive host raccoons foraging along streams.en
dc.description.sponsorshipNational Science Foundation SBE-0244916, DEB-0918960, DEB-0918656en
dc.description.sponsorshipRadford University College of Science and Technology Faculty Researchen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationLisa K. Belden, William E. Peterman, Stephen A. Smith, Lauren R. Brooks, E. F. Benfield, Wesley P. Black, Zhaomin Yang, and Jeremy M. Wojdak (2012. "Metagonimoides oregonensis (Heterophyidae: Digenea) Infection In Pleurocerid Snails and Desmognathus quadramaculatus Salamander Larvae In Southern Appalachian Streams," Journal of Parasitology, Vol. 98, No. 4, pp. 760-767. doi: http://dx.doi.org/10.1645/GE-2986.1en
dc.identifier.doihttps://doi.org/10.1645/ge-2986.1en
dc.identifier.issn0022-3395en
dc.identifier.urihttp://hdl.handle.net/10919/49057en
dc.identifier.urlhttp://www.bioone.org/doi/abs/10.1645/GE-2986.1en
dc.language.isoenen
dc.publisherAmerican Society of Parasitologyen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjecthelminth-parasitesen
dc.subjectgreen frogsen
dc.subjectmegalodiscus-temperatusen
dc.subjectprocyon-lotoren
dc.subjectlife-cycleen
dc.subjecttadpolesen
dc.subjecthosten
dc.subjectplatyhelminthesen
dc.subjecttransmissionen
dc.subjectparasitologyen
dc.titleMetagonimoides oregonensis (heterophyidae: digenea) infection in pleurocerid snails and desmognathus quadramaculatus salamander larvae in southern Appalachian streamsen
dc.title.serialJournal of Parasitologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
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