Elucidation of resistance signaling and identification of powdery mildew resistant mapping loci (ClaPMR2) during watermelon-Podosphaera xanthii interaction using RNA-Seq and whole-genome resequencing approach

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dc.contributor.authorMandal, Mihir Kumaren
dc.contributor.authorSuren, Haktanen
dc.contributor.authorKousik, Chandrasekaren
dc.contributor.departmentForest Resources and Environmental Conservationen
dc.date.accessioned2021-03-12T13:28:14Zen
dc.date.available2021-03-12T13:28:14Zen
dc.date.issued2020-08-20en
dc.description.abstractWatermelon is an important vegetable crop and is widely cultivated in USA with an approximate global production of >100 million tons. Powdery mildew (PM) caused by Podosphaera xanthii is a major production-limiting factor on watermelon and other cucurbits. Numerous PM and multiple disease resistant (MDR) watermelon germplasm lines have been developed by the USDA in Charleston, SC. To gain a better understanding of the innate and activated molecular defense mechanisms involved during compatible and incompatible PM-watermelon interactions, we inoculated PM susceptible (USVL677-PMS) and resistant (USVL531-MDR) watermelon plants with 10(5) conidia ml(-1) of P. xanthii. RNA-seq profiling was done on leaf samples collected at 0, 1, 3, and 8 days post inoculation (DPI). A total of 2,566 unique differentially expressed genes (DEGs) were identified between compatible and incompatible interactions with P. xanthii. The compatible interactions resulted in distinct plant gene activation (>twofold unique transcripts, 335:191:1762en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesThis research was funded in part by a SCRI Vegetable Grafting Grant award 2016-1498-08 and the SCRI CuCAP Grant award 2015-51181-24285 to C. S. Kousik. Mihir Mandal acknowledges the fund provided by ORISE. The whole genome sequencing of USVL531-MDR and USVL677-PMS was performed at the Duke Center for Genomic and Computational Biology, Durham, NC, USA with Illumina HiSeq 4000 (Illumina, Inc., San Diego, CA, USA) while RIL lines were performed at Novogene Corporation, Sacramento, CA, USA using HiSeq X Ten platform. The authors would also like to acknowledge Dr. Jason Holliday, Associate Professor, Department of Forest Resources and Environmental Conservation, Virginia Tech for his valuable suggestions on analyzing our transcriptomic and whole genome sequencing data. The authors would also like to acknowledge the use of Virginia Tech Advanced Research Computing Center for clustering and bioinformatic sequence data analysis. The authors wish to acknowledge the technical assistance provided by Jennifer Ikerd and summer undergraduates in conducting many of these experiments. We also thank Dr. William Rutter, Plant Pathologist, USVL-USDA Charleston for critical review and suggestions to improve the manuscript prior to submission. Disclaimer: The use of trade, firm, or corporation names in this publication is for the convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agriculture Research Service of any product or service to the exclusion of others that may also be suitable.en
dc.description.sponsorshipSCRI Vegetable Grafting Grant award [2016-1498-08]; SCRI CuCAP Grant award [2015-51181-24285]; ORISEen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41598-020-70932-zen
dc.identifier.issn2045-2322en
dc.identifier.issue1en
dc.identifier.other14038en
dc.identifier.pmid32820191en
dc.identifier.urihttp://hdl.handle.net/10919/102666en
dc.identifier.volume10en
dc.language.isoenen
dc.rightsPublic Domainen
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/en
dc.titleElucidation of resistance signaling and identification of powdery mildew resistant mapping loci (ClaPMR2) during watermelon-Podosphaera xanthii interaction using RNA-Seq and whole-genome resequencing approachen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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