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Application of Exogenous dsRNAs-induced RNAi in Agriculture: Challenges and Triumphs

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dc.contributor.authorDas, Protiva Ranien
dc.contributor.authorSherif, Sherif M.en
dc.date.accessioned2021-10-04T14:44:37Zen
dc.date.available2021-10-04T14:44:37Zen
dc.date.issued2020-06-25en
dc.date.updated2021-10-04T14:44:32Zen
dc.description.abstractIn recent years, RNA interference (RNAi) machinery has widely been explored by plant biologists for its potential applications in disease management, plant development, and germplasm improvement. RNAi-based technologies have mainly been applied in the form of transgenic plant generation and host-induced-gene-silencing (HIGS). However, the approval of RNAi-based transgenic plants has always been challenging due to the proclaimed concerns surrounding their impacts on human health and the environment. Lately, exogenous applications of double-stranded RNAs (dsRNAs), short interfering RNAs (siRNAs), and hairpin RNAs (hpRNAs) has emerged as another technology that could be regarded as more eco-friendly, sustainable, and publicly acceptable than genetic transformation. Inside the plant cell, dsRNAs can undergo several steps of processing, which not only triggers RNAi machinery but may also involve transitive and systemic silencing, as well as epigenetic modifications. Therefore, along with the considerations of proper exogenous applications of dsRNAs, defining their final destination into plant cells is highly relevant. In this review, we highlighted the significance of several factors that affect dsRNA-induced gene silencing, the fate of exogenous dsRNAs in the plant cell, and the challenges surrounding production technologies, cost-effectiveness, and dsRNAs stability under open-field conditions. This review also provided insights into the potential applications of exogenous dsRNAs in plant protection and crop improvement.en
dc.description.versionPublished versionen
dc.format.extent12 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 946 (Article number)en
dc.identifier.doihttps://doi.org/10.3389/fpls.2020.00946en
dc.identifier.eissn1664-462Xen
dc.identifier.issn1664-462Xen
dc.identifier.pmid32670336en
dc.identifier.urihttp://hdl.handle.net/10919/105154en
dc.identifier.volume11en
dc.language.isoenen
dc.publisherFrontiersen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000551760900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectPlant Sciencesen
dc.subjectexogenous dsRNAsen
dc.subjectplant RNAien
dc.subjectapplication methoden
dc.subjectdelivery techniqueen
dc.subjectsymplastic movementen
dc.subjectapoplastic movementen
dc.subjectagricultural traitsen
dc.subjectCELL-TO-CELLen
dc.subjectDISTANCE TRANSPORTen
dc.subjectCHALCONE SYNTHASEen
dc.subjectSIRNA BIOGENESISen
dc.subjectPLANTSen
dc.subjectINTERFERENCEen
dc.subjectMECHANISMSen
dc.subjectMOVEMENTen
dc.subjectSUPPRESSIONen
dc.subjectTRAFFICKINGen
dc.subject0607 Plant Biologyen
dc.titleApplication of Exogenous dsRNAs-induced RNAi in Agriculture: Challenges and Triumphsen
dc.title.serialFrontiers in Plant Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournalen
dcterms.dateAccepted2020-06-10en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Alson H. Smith, Jr. ARECen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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