The Root Lesion Nematode Effector Ppen10370 Is Essential for Parasitism of Pratylenchus penetrans

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dc.contributor.authorVieira, Pauloen
dc.contributor.authorVicente, Cláudia S. L.en
dc.contributor.authorBranco, Jordanaen
dc.contributor.authorBuchan, Garyen
dc.contributor.authorMota, Manuelen
dc.contributor.authorNemchinov, Lev G.en
dc.date.accessioned2021-11-19T14:12:20Zen
dc.date.available2021-11-19T14:12:20Zen
dc.date.issued2021-06en
dc.description.abstractThe root lesion nematode Pratylenchus penetrans is a migratory species that attacks a broad range of crops. Like other plant pathogens, P. penetrans deploys a battery of secreted protein effectors to manipulate plant hosts and induce disease. Although several candidate effectors of P. penetrans have been identified, detailed mechanisms of their functions and particularly their host targets remain largely unexplored. In this study, a repertoire of candidate genes encoding pioneer effectors of P. penetrans was amplified from mixed life stages of the nematode, and candidate effectors were cloned and subjected to transient expression in a heterologous host, Nicotiana benthamiana, using potato virus X-based gene vector. Among seven analyzed genes, the candidate effector designated as Ppen10370 triggered pleiotropic phenotypes substantially different from those produced by wild type infection. Transcriptome analysis of plants expressing Ppen10370 demonstrated that observed phenotypic changes were likely related to disruption of core biological processes in the plant due to effector-originated activities. Cross-species comparative analysis of Ppen10370 identified homolog gene sequences in five other Pratylenchus species, and their transcripts were found to be localized specifically in the nematode esophageal glands by in situ hybridization. RNA silencing of the Ppen10370 resulted in a significant reduction of nematode reproduction and development, demonstrating an important role of the esophageal gland effector for parasitism.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesThis project was supported by the United States Department of Agriculture Agricultural Research Service CRIS project number 804221000-300-00D, by the National Funds through Fundacao para a Ciencia e a Tecnologia (FCT, Foundation for Science and Technology) under the projects UIDB/05183/2020 and PTDC/AGR-PRO/2589/2014 (to M. Mota and J. Branco) and by a FCT postdoctoral fellowship SFRH/BPD/116030/2016 (to C. S. L. Vicente).en
dc.description.sponsorshipUnited States Department of Agriculture Agricultural Research Service CRIS project [804221000-300-00D]; Fundacao para a Ciencia e a Tecnologia (FCT, Foundation for Science and Technology) [UIDB/05183/2020, PTDC/AGR-PRO/2589/2014]; FCT postdoctoral fellowship [SFRH/BPD/116030/2016]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1094/MPMI-09-20-0267-Ren
dc.identifier.eissn1943-7706en
dc.identifier.issn0894-0282en
dc.identifier.issue6en
dc.identifier.pmid33400561en
dc.identifier.urihttp://hdl.handle.net/10919/106686en
dc.identifier.volume34en
dc.language.isoenen
dc.rightsPublic Domain (U.S.)en
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/en
dc.subjectnematode-plant interactionsen
dc.subjectnematode-induced plant genesen
dc.subjectnematode effectorsen
dc.subjecttransient expression using potato virus X (PVX)-based vectoren
dc.titleThe Root Lesion Nematode Effector Ppen10370 Is Essential for Parasitism of Pratylenchus penetransen
dc.title.serialMolecular Plant-Microbe Interactionsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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