Comparison of the Biomechanics of the Mouse Astrocytic Lamina Cribrosa Between Glaucoma and Optic Nerve Crush Models

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dc.contributor.authorKorneva, Arinaen
dc.contributor.authorKimball, Elizabeth C.en
dc.contributor.authorJohnson, Thomas V.en
dc.contributor.authorQuillen, Sarah E.en
dc.contributor.authorPease, Mary E.en
dc.contributor.authorQuigley, Harry A.en
dc.contributor.authorNguyen, Thao D.en
dc.date.accessioned2024-02-21T13:13:06Zen
dc.date.available2024-02-21T13:13:06Zen
dc.date.issued2023-12-13en
dc.description.abstractPURPOSE. The strain response of the mouse astrocytic lamina (AL) to an ex vivo mechanical test was compared between two protocols: eyes that underwent sustained intraocular pressure (IOP) increase and eyes after optic nerve crush. METHODS. Chronic IOP elevation was induced by microbead injection or the optic nerve was crushed in mice with widespread green fluorescence. After 3 days or 6 weeks, eyes were inflation tested by a published method of two-photon fluorescence to image the AL. Digital volume correlation was used to calculate strains. Optic nerve axon damage was also evaluated. RESULTS. In the central AL but not the peripheral AL, four strains were greater in eyes at the 3-day glaucoma time point than control (P from 0.029 to 0.049, n = 8 eyes per group). Also, at this time point, five strains were greater in the central AL compared to the peripheral AL (P from 0.041 to 0.00003). At the 6-week glaucoma time point, the strains averaged across the specimen, in the central AL, and the peripheral AL were indistinguishable from the respective controls. Strains were not significantly different between controls and eyes 3 days or 6 weeks after crush (n = 8 and 16). CONCLUSIONS. We found alterations in the ex vivo mechanical behavior in eyes from mice with experimental glaucoma but not in those with crushed optic nerves. The results of this study demonstrate that significant axon injury does not directly affect mechanical behavior of the astrocytic lamina.en
dc.description.versionPublished versionen
dc.format.extentPages 14en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1167/iovs.64.15.14en
dc.identifier.eissn1552-5783en
dc.identifier.issn0146-0404en
dc.identifier.issue15en
dc.identifier.orcidKorneva, Arina [0000-0001-5080-4224]en
dc.identifier.other2793103 (PII)en
dc.identifier.pmid38088825en
dc.identifier.urihttps://hdl.handle.net/10919/118073en
dc.identifier.volume64en
dc.language.isoenen
dc.publisherAssociation for Research in Vision and Ophthalmology (ARVO)en
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/38088825en
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subject.meshOptic Nerveen
dc.subject.meshScleraen
dc.subject.meshAnimalsen
dc.subject.meshMiceen
dc.subject.meshOptic Nerve Injuriesen
dc.subject.meshGlaucomaen
dc.subject.meshIntraocular Pressureen
dc.subject.meshBiomechanical Phenomenaen
dc.titleComparison of the Biomechanics of the Mouse Astrocytic Lamina Cribrosa Between Glaucoma and Optic Nerve Crush Modelsen
dc.title.serialInvestigative Ophthalmology and Visual Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournal Articleen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/Biomedical Engineering and Mechanicsen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Facultyen

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