Long-term storage of liquid boar spermatozoa
| dc.contributor.author | Underwood, Charles Raymond | en |
| dc.contributor.department | Animal Science (Reproductive Physiology) | en |
| dc.date.accessioned | 2017-03-10T21:56:00Z | en |
| dc.date.available | 2017-03-10T21:56:00Z | en |
| dc.date.issued | 1981 | en |
| dc.description.abstract | This study was conducted to: (a) determine the optimum extender system for two extenders that will maintain the highest level of cellular integrity when stored at either 5C or 15C for a minimum of 72 hr; (b) evaluate the fertilizing capacity of stored spermatozoa using the optimum extender system; (c) critically analyze enzymatic and morphological changes associated with storage and aging of boar spermatozoa; and (d) characterize properties of boar spermatozoa important to fertilization. The least-squares means for the percentage of normal apical ridge acrosomes were significantly affected by ejaculate within boar (P< .001), boar x extender (P< .05), boar x storage temperature (P< .01), extender x storage temperature (P<.01), extender x cooling rate (P< .05) and antibiotic x storage temperature (P< .01). The regression equations of storage time x treatment effect were reported. The least-squares means for acrosin activity were significantly affected by ejaculate within boar (P<.001), ejaculate within boar x antibiotic (P< .05), ejaculate within boar x extender (P<. 001), ejaculate within boar x storage temperature and extender x storage temperature (P< .05). The regression of storage time x storage temperature was reported. The least-squares means for progressive forward motility were significantly affected by extender (P< .05), antibiotic (P< .05), ejaculate within boar (P< .001), antibiotic x extender (P< .05) and extender x storage temperature (P< .01). The regressions of storage time x treatment effects were reported. The least-squares means for vibrational and/or rotational motility were affected by ejaculate within boar (P< .001), ejaculate within boar x extender (P<.001), ejaculate within boar x cooling rate (P<.05), antibiotic x extender and extender x storage temperature (P< .01). The optimum extender system consisted of Purdue extender containing penicillin/streptomycin and cooled to a 15C storage temperature in 4 hours. This extender system maintained a 70% minimum fertilization rate for 84 hours. | en |
| dc.description.degree | Ph. D. | en |
| dc.format.extent | vii, 137, [2] leaves | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.uri | http://hdl.handle.net/10919/76584 | en |
| dc.language.iso | en_US | en |
| dc.publisher | Virginia Polytechnic Institute and State University | en |
| dc.relation.isformatof | OCLC# 7960064 | en |
| dc.rights | In Copyright | en |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | en |
| dc.subject.lcc | LD5655.V856 1981.U862 | en |
| dc.subject.lcsh | Frozen semen | en |
| dc.subject.lcsh | Boars | en |
| dc.title | Long-term storage of liquid boar spermatozoa | en |
| dc.type | Dissertation | en |
| dc.type.dcmitype | Text | en |
| thesis.degree.discipline | Animal Science (Reproductive Physiology) | en |
| thesis.degree.grantor | Virginia Polytechnic Institute and State University | en |
| thesis.degree.level | doctoral | en |
| thesis.degree.name | Ph. D. | en |
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