Levels of Germination Proteins in Bacillus subtilis Dormant, Superdormant, and Germinating Spores

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dc.contributor.authorChen, Yanen
dc.contributor.authorRay, W. Keithen
dc.contributor.authorHelm, Richard F.en
dc.contributor.authorMelville, Stephen B.en
dc.contributor.authorPopham, David L.en
dc.contributor.departmentBiochemistryen
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2017-02-06T16:15:07Zen
dc.date.available2017-02-06T16:15:07Zen
dc.date.issued2014-04-21en
dc.description.abstractBacterial endospores exhibit extreme resistance to most conditions that rapidly kill other life forms, remaining viable in this dormant state for centuries or longer. While the majority of Bacillus subtilis dormant spores germinate rapidly in response to nutrient germinants, a small subpopulation termed superdormant spores are resistant to germination, potentially evading antibiotic and/or decontamination strategies. In an effort to better understand the underlying mechanisms of superdormancy, membrane-associated proteins were isolated from populations of B. subtilis dormant, superdormant, and germinated spores, and the relative abundance of 11 germination-related proteins was determined using multiplereaction-monitoring liquid chromatography-mass spectrometry assays. GerAC, GerKC, and GerD were significantly less abundant in the membrane fractions obtained from superdormant spores than those derived from dormant spores. The amounts of YpeB, GerD, PrkC, GerAC, and GerKC recovered in membrane fractions decreased significantly during germination. Lipoproteins, as a protein class, decreased during spore germination, while YpeB appeared to be specifically degraded. Some protein abundance differences between membrane fractions of dormant and superdormant spores resemble protein changes that take place during germination, suggesting that the superdormant spore isolation procedure may have resulted in early, non-committal germination-associated changes. In addition to low levels of germinant receptor proteins, a deficiency in the GerD lipoprotein may contribute to heterogeneity of spore germination rates. Understanding the reasons for superdormancy may allow for better spore decontamination procedures.en
dc.description.versionPublished versionen
dc.format.extent10 pagesen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0095781en
dc.identifier.issn1932-6203en
dc.identifier.issue4en
dc.identifier.urihttp://hdl.handle.net/10919/74940en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherPLOSen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000335227400127&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectclostridium-perfringens sporesen
dc.subjectdipicolinic acid uptakeen
dc.subjectinner membraneen
dc.subjectspova proteinsen
dc.subjectcontrast microscopyen
dc.subjectnutrient receptorsen
dc.subjectlocalizationen
dc.subjectresistanceen
dc.subjectoperonen
dc.subjectgerden
dc.titleLevels of Germination Proteins in Bacillus subtilis Dormant, Superdormant, and Germinating Sporesen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/Biological Sciencesen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen

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