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A novel species of RNA virus associated with root lesion nematode Pratylenchus penetrans

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dc.contributor.authorVieira, Pauloen
dc.contributor.authorNemchinov, Lev G.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2020-03-03T17:54:07Zen
dc.date.available2020-03-03T17:54:07Zen
dc.date.issued2019-04en
dc.description.abstractThe root lesion nematode Pratylenchus penetrans is a migratory species that attacks a broad range of plants. While analysing transcriptomic datasets of P. penetrans, we have identified a full-length genome of an unknown positive-sense singlestranded RNA virus, provisionally named root lesion nematode virus 1 (RLNV1). The 8614-nucleotide genome sequence encodes a single large polyprotein with conserved domains characteristic for the families Picornaviridae, Iflaviridae and Secoviridae of the order Picornavirales. Phylogenetic, BLAST and domain search analyses showed that RLNV1 is a novel species, most closely related to the recently identified sugar beet cyst nematode virus 1 and potato cyst nematode picornalike virus. In situ hybridization with a DIG-labelled DNA probe confirmed the presence of the virus within the nematodes. A negative-strand-specific RT-PCR assay detected RLNV1 RNA in nematode total RNA samples, thus indicating that viral replication occurs in P. penetrans. To the best of our knowledge, RLNV1 is the first virus identified in Pratylenchus spp.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesThis research was supported by the United States Department of Agriculture, Agricultural Research Service.en
dc.description.sponsorshipUnited States Department of Agriculture, Agricultural Research ServiceUnited States Department of Agriculture (USDA)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1099/jgv.0.001246en
dc.identifier.eissn1465-2099en
dc.identifier.issn0022-1317en
dc.identifier.issue4en
dc.identifier.pmid30869581en
dc.identifier.urihttp://hdl.handle.net/10919/97121en
dc.identifier.volume100en
dc.language.isoenen
dc.rightsCC0 1.0 Universalen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectroot lesion nematodeen
dc.subjectPratylenchus penetransen
dc.subjectpositive-sense single-stranded RNA virusen
dc.titleA novel species of RNA virus associated with root lesion nematode Pratylenchus penetransen
dc.title.serialJournal of General Virologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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