Diversity of unique, nonmycorrhizal endophytic fungi in cultivated Phalaenopsis orchids: A pilot study

dc.contributor.authorWatkinson, Jonathan I.en
dc.contributor.authorWinkel, Brenda S. J.en
dc.date.accessioned2025-01-31T20:40:24Zen
dc.date.available2025-01-31T20:40:24Zen
dc.date.issued2024-05-17en
dc.description.abstractOrchids comprise one of the largest, most diverse, and most broadly distributed families of flowering plants and contribute significantly to habitat biodiversity. One key aspect of orchid growth and development is the formation of mycorrhizal symbioses with compatible endophytic fungi, which are maintained throughout the life of the plant. Substantial efforts to identify the fungi that form mycorrhizal symbioses across a range of orchid species have often also uncovered numerous nonmycorrhizal, endophytic fungi. These fungi could also have significant effects on orchid growth and development and are beginning to be analyzed more closely, particularly in wild species. The role of endophytic fungi in the production, distribution, and continued growth by the hobbyist of orchids is not known. As an initial step toward characterizing nonmycorrhizal endophytic fungi associated with cultivated orchids, we undertook a survey of fungi residing within roots of Phalaenopsis plants growing in home environments. Sequence analysis of ITS regions amplified from total DNA isolated from roots allowed rapid identification of endophytic fungi to the class level and may offer a useful initial screening method for beneficial species, for example, in horticultural settings. ITS-PCR sequences subsequently obtained from individual fungi cultured from surface-sterilized orchid roots corroborated the findings of the initial screen, while also providing a more complete characterization of the array of fungal taxa that were present. Although lower in diversity than has been reported for orchids growing in the wild, these endophytes have the potential to substantially enhance the growth and disease resistance of horticultural orchids.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1002/pei3.10146en
dc.identifier.eissn2575-6265en
dc.identifier.issn2575-6265en
dc.identifier.issue3en
dc.identifier.orcidWatkinson, Jonathan [0000-0002-8163-9927]en
dc.identifier.orcidWinkel, Brenda [0000-0003-3481-024X]en
dc.identifier.otherPMC11101157en
dc.identifier.otherPEI310146 (PII)en
dc.identifier.pmid38764601en
dc.identifier.urihttps://hdl.handle.net/10919/124466en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/38764601en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectascomycotaen
dc.subjectendophytesen
dc.subjecthorticulturalen
dc.subjectphytochemicalen
dc.subjectscreening methoden
dc.titleDiversity of unique, nonmycorrhizal endophytic fungi in cultivated <i>Phalaenopsis</i> orchids: A pilot studyen
dc.title.serialPlant-Environment Interactionsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournal Articleen
dcterms.dateAccepted2024-05-02en
pubs.organisational-groupVirginia Techen
pubs.organisational-groupVirginia Tech/Scienceen
pubs.organisational-groupVirginia Tech/Science/Biological Sciencesen
pubs.organisational-groupVirginia Tech/All T&R Facultyen
pubs.organisational-groupVirginia Tech/Science/COS T&R Facultyen

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Diversity of unique, nonmycorrhizal endophytic fungi in cultivated &lt;i&gt;Phalaenopsis&lt;i&gt; orchids A pilot study.pdf
Size:
908.97 KB
Format:
Adobe Portable Document Format
Description:
Published version
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.5 KB
Format:
Plain Text
Description: