Characterization of Glutamate-Mediated Hormonal Regulatory Pathway of the Drought Responses in Relation to Proline Metabolism in Brassica napus L.

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dc.contributor.authorLa, Van Hienen
dc.contributor.authorLee, Bok-Ryeen
dc.contributor.authorIslam, Md Tabibulen
dc.contributor.authorAl Mamun, Mden
dc.contributor.authorPark, Sang-Hyunen
dc.contributor.authorBae, Dong-Wonen
dc.contributor.authorKim, Tae-Hwanen
dc.contributor.departmentVirginia Agricultural Experiment Stationen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2020-09-02T12:44:30Zen
dc.date.available2020-09-02T12:44:30Zen
dc.date.issued2020-04en
dc.description.abstractProline metabolism influences the metabolic and/or signaling pathway in regulating plant stress responses. This study aimed to characterize the physiological significance of glutamate (Glu)-mediated proline metabolism in the drought stress responses, focusing on the hormonal regulatory pathway. The responses of cytosolic Ca2+ signaling, proline metabolism, and redox components to the exogenous application of Glu in well-watered or drought-stressed plants were interpreted in relation to endogenous hormone status and their signaling genes. Drought-enhanced level of abscisic acid (ABA) was concomitant with the accumulation of ROS and proline, as well as loss of reducing potential, which was assessed by measuring NAD(P)H/NAD(P)(+) and GSH/GSSG ratios. Glu application to drought-stressed plants increased both salicylic acid (SA) and cytosolic Ca2+ levels, with the highest expression of calcium-dependent protein kinase (CPK5) and salicylic acid synthesis-related ICS1. The SA-enhanced CPK5 expression was closely associated with further enhancement of proline synthesis-related genes (P5CS1, P5CS2, and P5CR) expression and a reset of reducing potential with enhanced expression of redox regulating genes (TRXh5 and GRXC9) in a SA-mediated NPR1- and/or PR1-dependent manner. These results clearly indicate that Glu-activated interplay between SA- and CPK5-signaling as well as Glu-enhanced proline synthesis are crucial in the amelioration of drought stress in Brassica napus.en
dc.description.notesThis work was funded by a grant from the National Research Foundation (NRF-2019R1A2C1089340).en
dc.description.sponsorshipNational Research Foundation [NRF-2019R1A2C1089340]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3390/plants9040512en
dc.identifier.eissn2223-7747en
dc.identifier.issue4en
dc.identifier.other512en
dc.identifier.pmid32316109en
dc.identifier.urihttp://hdl.handle.net/10919/99892en
dc.identifier.volume9en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectcalcium signalingen
dc.subjectglutamateen
dc.subjectproline synthesisen
dc.subjectredoxen
dc.subjectsalicylic aciden
dc.titleCharacterization of Glutamate-Mediated Hormonal Regulatory Pathway of the Drought Responses in Relation to Proline Metabolism in Brassica napus L.en
dc.title.serialPlantsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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