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Oscillatory Dynamics of Cell Cycle Proteins in Single Yeast Cells Analyzed by Imaging Cytometry

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dc.contributor.authorBall, David A.en
dc.contributor.authorMarchand, Julieen
dc.contributor.authorPoulet, Magalyen
dc.contributor.authorBaumann, William T.en
dc.contributor.authorChen, Katherine C.en
dc.contributor.authorTyson, John J.en
dc.contributor.authorPeccoud, Jeanen
dc.date.accessioned2016-12-09T21:26:03Zen
dc.date.available2016-12-09T21:26:03Zen
dc.date.issued2011-10-26en
dc.description.abstractProgression through the cell division cycle is orchestrated by a complex network of interacting genes and proteins. Some of these proteins are known to fluctuate periodically during the cell cycle, but a systematic study of the fluctuations of a broad sample of cell-cycle proteins has not been made until now. Using time-lapse fluorescence microscopy, we profiled 16 strains of budding yeast, each containing GFP fused to a single gene involved in cell cycle regulation. The dynamics of protein abundance and localization were characterized by extracting the amplitude, period, and other indicators from a series of images. Oscillations of protein abundance could clearly be identified for Cdc15, Clb2, Cln1, Cln2, Mcm1, Net1, Sic1, and Whi5. The period of oscillation of the fluorescently tagged proteins is generally in good agreement with the inter-bud time. The very strong oscillations of Net1 and Mcm1 expression are remarkable since little is known about the temporal expression of these genes. By collecting data from large samples of single cells, we quantified some aspects of cell-to-cell variability due presumably to intrinsic and extrinsic noise affecting the cell cycle.en
dc.description.sponsorshipThis work was supported by grant R01-GM078989 from the National Institutes of Health and National Science Foundation Award DBI-0963988. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.description.versionPublished versionen
dc.format.extent12 pagesen
dc.identifier.citationBall DA, Marchand J, Poulet M, Baumann WT, Chen KC, et al. (2011) Oscillatory Dynamics of Cell Cycle Proteins in Single Yeast Cells Analyzed by Imaging Cytometry. PLoS ONE 6(10): e26272. doi:10.1371/journal.pone.0026272en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0026272en
dc.identifier.issn1932-6203en
dc.identifier.issue10en
dc.identifier.urihttp://hdl.handle.net/10919/73618en
dc.identifier.volume6en
dc.languageEnglishen
dc.publisherPLOSen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000296519600012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectb-type cyclinsen
dc.subjectsaccharomyces-cerevisiaeen
dc.subjectbudding yeasten
dc.subjectgene-expressionen
dc.subjectmitotic exiten
dc.subjects-phaseen
dc.subjectspindle checkpointen
dc.subjectglobal analysisen
dc.subjectg1 cyclinsen
dc.subjectanaphaseen
dc.titleOscillatory Dynamics of Cell Cycle Proteins in Single Yeast Cells Analyzed by Imaging Cytometryen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineering/Electrical and Computer Engineeringen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/Biological Sciencesen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Distinguished Professorsen

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