Whole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosis

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dc.contributor.authorLiu, Lingbinen
dc.contributor.authorXiao, Qihaien
dc.contributor.authorGilbert, Elizabeth R.en
dc.contributor.authorCui, Zhifuen
dc.contributor.authorZhao, Xiaolingen
dc.contributor.authorWang, Yanen
dc.contributor.authorYin, Huadongen
dc.contributor.authorLi, Diyanen
dc.contributor.authorZhang, Haihanen
dc.contributor.authorZhu, Qingen
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2018-12-12T20:05:54Zen
dc.date.available2018-12-12T20:05:54Zen
dc.date.issued2018-05-08en
dc.description.abstractBroodiness in laying hens results in atrophy of the ovary and consequently decreases productivity. However, the regulatory mechanisms that drive ovary development remain elusive. Thus, we collected atrophic ovaries (AO) from 380-day-old broody chickens (BC) and normal ovaries (NO) from evenaged egg-laying hens (EH) for RNA sequencing. We identified 3,480 protein-coding transcripts that were differentially expressed (DE), including 1,719 that were down-regulated and 1,761 that were up-regulated in AO. There were 959 lncRNA transcripts that were DE, including 56 that were downregulated and 903 that were up-regulated. Among the116 miRNAs that were DE, 79 were downregulated and 37 were up-regulated in AO. Numerous DE protein-coding transcripts and target genes for miRNAs/lncRNAs were significantly enriched in reproductive processes, cell proliferation, and apoptosis pathways. A miRNA-intersection gene-pathway network was constructed by considering target relationships and correlation of the expression levels between ovary development-related genes and miRNAs. We also constructed a competing endogenous RNA (ceRNA) network by integrating competing relationships between protein-coding genes and lncRNA transcripts, and identified several lncRNA transcripts predicted to regulate the CASP6, CYP1B1, GADD45, MMP2, and SMAS2 genes. In conclusion, we discovered protein-coding genes, miRNAs, and lncRNA transcripts that are candidate regulators of ovary development in broody chickens.en
dc.description.notesThis work was financially supported by the China Agriculture Research System (CARS-41).en
dc.description.sponsorshipChina Agriculture Research System [CARS-41]en
dc.format.extent14en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41598-018-25103-6en
dc.identifier.issn2045-2322en
dc.identifier.other7231en
dc.identifier.pmid29739971en
dc.identifier.urihttp://hdl.handle.net/10919/86356en
dc.identifier.volume8en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectlong noncoding rnasen
dc.subjectgenome-wide identificationen
dc.subjectcell-proliferationen
dc.subjectgene-expressionen
dc.subjectgranulosa-cellsen
dc.subjecttransitionen
dc.subjectbroodinessen
dc.subjectpolymorphismsen
dc.subjectmechanismsen
dc.subjectmicrornasen
dc.titleWhole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosisen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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