Browsing by Author "Moore, Ignacio T."

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    Abiotic Factors Underlying Stress Hormone Level Variation Among Larval Amphibians
    Chambers, David L. (Virginia Tech, 2009-03-31)
    Anthropogenic disturbances can alter the abiotic composition of freshwater systems. These compositional changes can act as physiological stressors towards system inhabitants. However, little is known about how these altered abiotic factors influence stress hormones (corticosterone) in larval amphibians. Throughout the following chapters, I examined the effects of several abiotic factors on baseline and stress-induced corticosterone levels in the larvae of four amphibian species: Jefferson salamander (Ambystoma jeffersonianum), spotted salamander (A. maculatum), wood frog (Rana sylvatica), and grey treefrog (Hyla versicolor). Chapter II examined corticosterone level differences throughout development in A. jeffersonianum and R. sylvatica larvae under field, mesocosm, and laboratory venues. Baseline corticosterone levels in R. sylvatica increased near metamorphic climax in all venues, but not in A. jeffersonianum. Rather, baseline corticosterone levels differed with respect to venue throughout development in A. jeffersonianum. Chapter III examined corticosterone level differences among free-living A. jeffersonianum populations and possible abiotic factors underlying these hormone differences. Corticosterone levels significantly differed across populations. Increased baseline corticosterone levels significantly correlated to low pH. There was also a trend for increased baseline corticosterone levels to be positively correlated with chloride levels and negatively correlated with conductivity. Chapter IV examined the effects of laboratory manipulated pH on corticosterone levels in A. jeffersonianum, A. maculatum, R. sylvatica, and H. versicolor. There was a significant correlation between increased baseline corticosterone levels to low pH in all four species. Prey consumption (in both Ambystoma species) and survival (in A. jeffersonianum, A. maculatum, and R. sylvatica) were also negatively correlated to low pH. Chapter V examined the effects of increased conductivity on corticosterone levels in A. jeffersonianum, R. sylvatica, and H. versicolor. Increased conductivity exposure significantly correlated to increased baseline corticosterone levels in A. jeffersonianum and R. sylvatica. Prey consumption in A. jeffersonianum was also negatively correlated to increased conductivity. My dissertation shows that abiotic factors, such as pH and conductivity, can influence corticosterone levels in larval amphibians. These results suggest that corticosterone levels in larval amphibians may be a suitable biomarker reflective of altered freshwater habitat quality. However, my results also suggest that one should use a high degree of caution when using corticosterone levels in larval amphibians as a means to infer the health status of a population.
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    Analysis and Management of UAV-Captured Images towards Automation of Building Facade Inspections
    Chen, Kaiwen (Virginia Tech, 2020-08-27)
    Building facades, serving mainly to protect occupants and structural components from natural forces, require periodic inspections for the detection and assessment of building façade anomalies. Over the past years, a growing trend of utilizing camera-equipped drones for periodical building facade inspection has emerged. Building façade anomalies, such as cracks and erosion, can be detected through analyzing drone-captured video, photographs, and infrared images. Such anomalies are known to have an impact on various building performance aspects, e.g., thermal, energy, moisture control issues. Current research efforts mainly focus on the design of drone flight schema for building inspection, 3D building model reconstruction through drone-captured images, and the detection of specific façade anomalies with these images. However, there are several research gaps impeding the improvement of automation level during the processes of building façade inspection with UAV (Unmanned Aerial Vehicle). These gaps are (1) lack effective ways to store multi-type data captured by drones with the connection to the spatial information of building facades, (2) lack high-performance tools for UAV-image analysis for the automated detection of building façade anomalies, and (3) lack a comprehensive management (i.e., storage, retrieval, analysis, and display) of large amounts and multi-media information for cyclic façade inspection. When seeking inspirations from nature, the process of drone-based facade inspection can be compared with caching birds' foraging food through spatial memory, visual sensing, and remarkable memories. This dissertation aims at investigating ways to improve the management of UAV-captured data and the automation level of drone-based façade anomaly inspection with inspirations from caching birds' foraging behavior. Firstly, a 2D spatial model of building façades was created in the geographic information system (GIS) for the registration and storage of UAV-images to assign façade spatial information to each image. Secondly, computational methods like computer vision and deep learning neural networks were applied to develop algorithms for automated extraction of visual features of façade anomalies within UAV-captured images. Thirdly, a GIS-based database was designed for the comprehensive management of heterogeneous inspection data, such as the spatial, multi-spectral, and temporal data. This research will improve the automation level of storage, retrieval, analysis, and documentation of drone-captured images to support façade inspection during a building's service lifecycle. It has promising potential for supporting the decision-making of early-intervention or maintenance strategies to prevent façade failures and improve building performance.
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    Analysis of the Optimal Duration of Behavioral Observations Based on an Automated Continuous Monitoring System in Tree Swallows (Tachycineta bicolor): Is One Hour Good Enough?
    Lendavi, Ádám; Akçay, Çağlar; Ouyang, Jenny Q.; Dakin, Roslyn; St. John, Prianka S.; Stanback, Mark; Moore, Ignacio T.; Bonier, Frances; Domalik, Alice D. (PLOS, 2015-11-11)
    Studies of animal behavior often rely on human observation, which introduces a number of limitations on sampling. Recent developments in automated logging of behaviors make it possible to circumvent some of these problems. Once verified for efficacy and accuracy, these automated systems can be used to determine optimal sampling regimes for behavioral studies. Here, we used a radio-frequency identification (RFID) system to quantify parental effort in a bi-parental songbird species: the tree swallow (Tachycineta bicolor). We found that the accuracy of the RFIDmonitoring systemwas similar to that of video-recorded behavioral observations for quantifying parental visits. Using RFID monitoring, we also quantified the optimum duration of sampling periods for male and female parental effort by looking at the relationship between nest visit rates estimated from sampling periods with different durations and the total visit numbers for the day. The optimum sampling duration (the shortest observation time that explained the most variation in total daily visits per unit time) was 1h for both sexes. These results show that RFID and other automated technologies can be used to quantify behavior when human observation is constrained, and the information fromthese monitoring technologies can be useful for evaluating the efficacy of human observation methods.
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    Assessing age, breeding stage, and mating activity as drivers of variation in the reproductive microbiome of female tree swallows
    Hernandez, Jessica; Hucul, Catherine; Reasor, Emily; Smith, Taryn; McGlothlin, Joel W.; Haak, David C.; Belden, Lisa K.; Moore, Ignacio T. (Wiley, 2021-07)
    Sexually transmitted microbes are hypothesized to influence the evolution of reproductive strategies. Though frequently discussed in this context, our understanding of the reproductive microbiome is quite nascent. Indeed, testing this hypothesis first re-quires establishing a baseline understanding of the temporal dynamics of the reproductive microbiome and of how individual variation in reproductive behavior and age influence the assembly and maintenance of the reproductive microbiome as a whole. Here, we ask how mating activity, breeding stage, and age influence the reproductive microbiome. We use observational and experimental approaches to explain variation in the cloacal microbiome of free- living, female tree swallows (Tachycineta bicolor). Using microsatellite- based parentage analyses, we determined the number of sires per brood (a proxy for female mating activity). We experimentally increased female sexual activity by administering exogenous 17ß-estradiol. Lastly, we used bacterial 16S rRNA amplicon sequencing to characterize the cloacal microbiome. Neither the number of sires per brood nor the increased sexual activity of females significantly influenced female cloacal microbiome richness or community structure. Female age, however, was positively correlated with cloacal microbiome richness and influenced overall community structure. A hypothesis to explain these patterns is that the effect of sexual activity and the number of mates on variation in the cloacal microbiome manifests over an individual's lifetime. Additionally, we found that cloacal microbiome alpha diversity (Shannon Index, Faith's phylogenetic distance) decreased and community structure shifted between breeding stages. This is one of few studies to document within-individual changes and age- related differences in the cloacal microbiome across successive breeding stages. More broadly, our results contribute to our understanding of the role that host life history and behavior play in shaping the cloacal microbiomes of wild birds.
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    Behavior, Physiology, and Reproduction of Urban and Rural Song Sparrows (Melospiza Melodia)
    Foltz, Sarah (Virginia Tech, 2015-06-01)
    Urban areas are a unique and growing habitat type. Animals living in this novel habitat are faced with new challenges, but may also encounter novel opportunities. Though urban animals have been observed to differ from their rural counterparts in a variety of behavioral and physiological traits, little is known about the specific features of urban areas that drive these differences and whether they are adaptive. Understanding this process is important from a conservation perspective and also to gain insight into how animals colonize novel habitats more generally. Using song sparrows (Melospiza melodia), a native songbird commonly found in urban areas, I explored responses to urbanization and the drivers and consequences of these responses with an eye toward understanding whether song sparrows had successfully adapted to urban habitats (Chapter I). I began by comparing body condition and levels of corticosterone, a hormone associated with energy management and the stress response in birds, between urban and rural populations (Chapter II). There was more variation across years than between habitats, suggesting that a variable environmental factor common to both habitats is the primary driver of these traits. I then compared territorial aggression levels and tested the effect of food availability on aggression (Chapter III). Fed rural birds and all urban birds had higher aggression levels than unfed rural birds, indicating that territorial aggression is related to resource availability in this species and that urban habitats may be perceived as more desirable. Finally, I looked for differences in reproductive timing and success and for relationships between reproductive success and aggression (Chapter IV). Higher reproductive success in urban populations, coupled with differences in the timing of successful nests between habitats, suggest differences in predation risk and predator community structure between habitats. In Chapter V, I synthesize my major findings and suggest directions for future research building on these results. I conclude that urban song sparrows differ from rural birds, that these differences are influenced by resource availability, and that urban habitats can potentially support stable song sparrow populations, though more research is necessary to determine the fitness impacts of specific traits that change with urbanization.
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    Behavioral Investigation of the Light-Dependent Magnetoreception Mechanism of Drosophila melanogaster
    Dommer, David H. (Virginia Tech, 2008-04-25)
    Use of a magnetic compass has been demonstrated in all major classes of vertebrates as well as several classes of invertebrates, and is proposed to involve a photo-induced radical pair mechanism (RPM). My dissertation research consisted of characterizing a magnetic compass in a model species, Drosophila melanogaster. Preliminary experiments were carried out with adult flies, however, due to the behavioral complexity of adult responses a new behavioral assay of magnetic compass orientation was developed using larval Drosophila that elicits a robust magnetic compass response in a trained magnetic direction. This manuscript describes experiments that were conducted showing that larval magnetic compass orientation: 1) demonstrates a complex 3-dimensional pattern of response consistent with a RPM; 2) is consistent with a receptor mechanism that utilizes short- and long-wavelength antagonistic photopigments, proposed to explain wavelength dependent effects in vertebrates (e.g. amphibians and birds); and 3) produces axially symmetrical patterns of response with respect to the geomagnetic field. Additionally, tests of adult Drosophila under low and high intensities of monochromatic long wavelength light revealed a similar behavioral response to varying intensities of monochromatic light as previously reported in migratory birds (E. rubecula). These findings indicate that the magnetic compass of larval Drosophila shares a common functional architecture and similar biophysical mechanism with that of at least some vertebrates (e.g. amphibians and possibly birds), suggesting that the magnetic compass of modern vertebrates may have evolved once in a common ancestor of these three lineages over 450 million years ago. Furthermore, findings indicating a spontaneous preference for magnetic directions in D. melanogaster larvae suggest that a light-dependent magnetoreception mechanism is more widespread in insects than was previously suspected. The development of a behavioral assay to study the light-dependent magnetic compass in an organism with a simple nervous system, a limited behavioral repertoire, and with the possibility of using the full power of modern molecular and genetic techniques holds considerable promise to increase our understanding of the biophysical mechanism(s) and neurophysiological structures underlying magnetic orientation in terrestrial animals.
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    Bidirectional interactions between behavior and disease in banded mongooses (Mungos mungo) infected with Mycobacterium mungi
    Fairbanks, Bonnie Marie (Virginia Tech, 2013-09-04)
    Behavior and disease interact bidirectionally and on multiple levels of host organization, and these interactions can have important consequences for population-level disease dynamics. I explored how behavior can both influence and respond to infectious disease in a banded mongoose population experiencing epidemics of tuberculosis (TB) caused by the bacterial pathogen Mycobacterium mungi in the M. tuberculosis complex (Alexander et al. 2010). Banded mongooses are highly social carnivores that live in troops of 5 to 65 individuals. Mycobacterium mungi appears to be primarily environmentally transmitted, but direct horizontal transmission cannot be ruled out. Approximately 10-20% of mongooses become diseased with TB each year in the study population in and around Chobe National Park, Botswana, and all mongooses with clinical signs of TB die within months. Characteristics of both banded mongooses and clinical TB provided a productive study system for exploring interactions between behavior and disease: first, free-living mongooses can be habituated and directly observed; second, the clinical signs of TB can be visually assessed non-invasively; and third, the mongooses' high sociality and egalitarianism provide a unique and ecologically relevant host social system for examining bidirectional interactions between behavior and infectious disease. I found that banded mongooses influenced and responded to disease through their behavior at both the individual and troop level, with possible implications for banded mongoose population and disease dynamics. Due to the environmental transmission of M. mungi, which appears to invade mongooses through breaks in the skin and nasal planum (Alexander et al. 2010), I focused on aggressive interactions as a potential risk factor for acquiring TB in this system. Troops with higher levels of aggression had more injuries, and at the individual level, injuries were a strong predictor of TB, suggesting that aggression may increase risk of disease by creating potential invasion sites for the pathogen. Troops were more aggressive when they foraged in garbage than when they foraged in other habitats, presumably due to the concentration of resources at this highly modified habitat. Overall, my results on how behavior can influence disease in this system suggest that anthropogenic supplementation of food, albeit inadvertent in this system, augments aggression levels in banded mongooses and may in turn lead to a higher incidence of TB. Second, I examined how behavior responds to disease in banded mongooses. Diseased individuals showed significantly lower activity and alertness, but intriguingly, did not show a reduction in overall social behaviors. Diseased individuals were less likely to disperse than healthy individuals, and healthy individuals with diseased troopmates may have been more likely to disperse than individuals without diseased troopmates. Despite this latter possible increase in dispersal in the presence of diseased conspecifics, diseased individuals were not avoided by their troopmates in daily social interactions. For example, diseased individuals were allogroomed at a higher than expected rate even though their reciprocation during allogrooming was approximately half that of healthy individuals. These interactions between behavior and disease have implications for banded mongoose troop and population dynamics, via changes in dispersal behavior and mortality, and can also affect disease dynamics, such as transmission rate. For example, changes to dispersal may affect the amount of inbreeding and outbreeding that occurs in this normally inbred species, and disease might be amplified in areas where aggression is increased by resource augmentation from humans. Additionally, the role that garbage plays in mongoose aggression suggests that humans may be inadvertently increasing disease incidence in this system, as well as in other taxa for which anthropogenic food augmentation may alter disease dynamics via changes in intraspecific aggression. This research sheds light on ways that behavior can influence and respond to disease that are often overlooked in disease ecology.
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    Biomimetic sonar design and the investigation of the role of peripheral dynamics for target classification in bat biosonar
    Sutlive, Joseph Vinson (Virginia Tech, 2020-12-17)
    The biosonar system of bats has many unique adaptations which allow for navigation in extremely cluttered environments. One such adaptation is the rapid motion of the pinna and noseleaf observed in certain families of old-world bats (Rhinolophidae and Hipposiderae). Little is known about the physical properties about this adaptation affects emitted pulses or incoming echoes. To explore the physical properties of biosonar systems utilizing dynamic peripheries, biomimetic sonar systems have been devised, which can be used to simulate the structural characteristics of the pinna and noseleaf geometry as well as the motor characteristics. Using this method, it was determined that the changing conformations of the biomimetic baffles were responsible for time-variant signatures in echoes. These signatures could be seen in echoes from a variety of both simple and complex target shapes. Then to further the capabilities of the device, an improved actuation system was devised using pneumatic actuation. This allowed for the baffles to make several unique motions as opposed to being restricted to one previously. It was also shown that the distinct motion profiles of the system led to distinct differences in the received acoustic signal. The features encoded by this system could lead to improvements in the development of improved sensing of smaller autonomous systems. GRANT INFORMATION: This work was supported by grants from the Office of Naval Research (ONR) and the Naval Engineering Education Consortium (NEEC). Additional support was provided by an East Asia and Pacific Summer Institutes (EAPSI) fellowship from the National Science Foundation (NSF).
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    Brain and Cognitive Consequences of Early-Life Immune System Challenge in a Songbird
    Campbell, Simone Alicia (Virginia Tech, 2016-05-17)
    Cognition, defined as the mechanism by which an animal acquires, processes, stores, and uses information present in the environment, is a trait that is sensitive to developmental conditions. Existing research supports the idea that the ability to develop and maintain cognitive abilities depends on the physiological condition of the individual, which can be influenced by the early environment. Alterations in maternal care, social stress, and malnutrition are some examples of environmental conditions that impact development and resulting cognitive abilities across taxa. The primary goal of this research was to determine whether immune system challenge during the critical song learning period in zebra finches (Taeniopygia guttata) would lead to long term negative impacts on song quality and learning, spatial learning, and neophobia. Immune challenge during this period of development did not produce long term impacts on learning or memory, nor did it lead to any changes in neophobic responses. However, birds that were hatched later in a clutch performed better on the motoric and spatial tasks, and were less neophobic. Future research in zebra finches that can describe the variation in song attributes as a function of hatching order would be a useful first step in determining a mechanistic link between hatch order and song learning outcomes.
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    The causes and consequences of variation in the cloacal microbiome of tree swallows (Tachycineta bicolor)
    Hernandez, Jessica (Virginia Tech, 2021-08-31)
    Animals are ecological landscapes that host communities of microbes often referred to as microbiomes. These microbes can be transferred between individuals when they come into contact, such as during mating. Microbes that reside in or on any aspect of a host that becomes exposed to the reproductive tract or gametes comprise the reproductive microbiome. These microbes within the reproductive microbiome are important to overall host biology because they can influence host reproductive function, and thus play a role in shaping host ecology, evolution, and fitness. Though previous work has revealed much about the impact of beneficial and pathogenic microbes within the reproductive tract, much is left to be learned from describing the dynamic nature of the reproductive microbiome, and ultimately, how it affects host fitness. For my dissertation, I asked questions regarding how and why reproductive microbiome diversity varies among individuals. For instance, does reproductive microbiome diversity vary with respect to the number of mates or mating activity? Does reproductive microbiome diversity vary with host age or breeding stage? Are there fitness consequences associated with differences in reproductive microbiome diversity? To explore these questions, I studied tree swallows (Tachycineta bicolor), a socially monogamous bird in which both females and males engage in extra-pair mating activity. I focused on the cloacal microbiome as it is the site of contact during mating, and thus where microbes can be sexually transferred between individuals. I found that social partners did not have more similar cloacal microbiome diversity compared to other individuals in the same population, and that cloacal microbiome diversity was similar between sexes (Chapter II). By combining an observational approach with a hormone implant manipulation, I found that neither the number of sires per brood nor the increased mating activity of females significantly influenced cloacal microbiome richness or community structure. However, female age and breeding stage did significantly correlate with cloacal microbiome richness and community structure (Chapter III). Based on these findings, I hypothesized that the effect of mating activity on variation in the cloacal microbiome may only be detectable over a female's lifetime, and not within a single breeding season. In addition, I found evidence for a relationship between lay date and cloacal microbiome structure, after controlling for age. And I found that older females lay earlier in the season compared to younger, first-time breeding females (Chapter IV). These results provide support for a relationship between lay date and the cloacal microbiome and highlight the importance of age to this relationship. Lastly, I discussed future steps that can be taken to extend the framework established by my dissertation research, and thereby gain further insight into the factors shaping the reproductive microbiome (Chapter V).
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    Characterization and Assessment of Transportation Diversity: Impacts on Mobility and Resilience Planning in Urban Communities
    Rahimi Golkhandan, Armin (Virginia Tech, 2020-06-25)
    A transportation system is a critical infrastructure that is key for mobility in any community. Natural hazards can cause failure in transportation infrastructure and impede its routine performance. Ecological systems are resilient systems that are very similar to transportation systems. Diversity is a fundamental factor in ecological resilience, and it is recognized as an important property of transportation resilience. However, quantifying transportation diversity remains challenging, which makes it difficult to understand the influence of diversity on transportation performance and resilience. Consequently, three studies are undertaken to remedy this circumstance. The first study develops a novel approach – inspired by biodiversity in ecological stability theory – to characterize and measure transportation diversity by its richness (availability) and evenness (distribution). This transportation diversity approach is then applied to New York City (NYC) at the zip code level using the GIS data of transportation modes. The results demonstrate the variation of transportation diversity across the city. The characterized inherent and augmented complementarities start to uncover the dynamics of modal compensation and to demonstrate how transportation diversity contributes to this phenomenon. Moreover, the NYC zip codes with low transportation diversity are mainly in hurricane evacuation zones that are more vulnerable. Consequently, low transportation diversity in these areas could affect their post-disaster mobility. In the second study, the influence of transportation diversity on post-disaster mobility is examined by investigating the patterns of mobility in New York City one month before and after Hurricane Sandy using Twitter data. To characterize pre- and post-Sandy mobility patterns, the locations that individuals visited frequently were identified and travel distance, the radius of gyration, and mobility entropy were measured. Individuals were grouped according to the transportation diversity of their frequently visited locations. The findings reveal that individuals that lived in or visited zip codes with higher transportation diversity mostly experienced less disturbance in their mobility patterns after Sandy and the recovery of their mobility patterns was faster. The results confirm that transportation diversity affects the resilience of individual post-disaster mobility. The approach used in this study is one of the first to examine the root causes of changes in mobility patterns after extreme events by linking transportation infrastructure diversity to post-disaster mobility. Finally, the third study employs the transportation diversity approach to investigate modal accessibility and social exclusion. Transportation infrastructure is a sociotechnical system and transport equity is crucial for access to opportunities and services such as jobs and infrastructure. The social exclusion caused by transport inequity could be intensified after natural disasters that can cause failure in a transportation system. One approach to determine transport equity is access to transportation modes. Common catchment area approaches to assess the equity of access to transportation modes cannot differentiate between the equity of access to modes in sub-regions of an area. The transportation diversity approach overcomes this shortcoming, and it is applied to all transportation modes in NYC zip codes to measure the equity of access. Zip codes were grouped in quartiles based on their transportation diversity. Using the American Community Survey data, a set of important socioeconomic and transport usage factors were compared in the quartile groups. The results indicated the relationship between transportation diversity and income, vehicle ownership, commute time, and commute mode. This relationship highlighted that social exclusion is linked with transport inequity. The results also revealed that the inequity of the transport system in zip codes with low transportation diversity affects poor individuals more than non-poor and the zip codes with a majority of black and Hispanic populations are impacted more. Further consideration of the impacts of Hurricanes Irene and Sandy in NYC shows that people in areas with a lower transportation diversity were affected more and the transport inequity in these areas made it difficult to cope with these disasters and caused post-disaster social exclusion. Therefore, enhancing transportation diversity should support transport equity and reduce social exclusion under normal situations and during extreme events. Together, these three studies illustrate the influence of transportation diversity on the resilience of this infrastructure. They highlight the importance of the provision and distribution of all transportation modes, their influence on mobility during normal situations and extreme events and their contribution toward mitigating social exclusion. Finally, these studies suggest that transportation diversity can contribute to more targeted and equitable transportation and community resilience planning, which should help decision-makers allocate scarce resources more effectively.
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    The Cloacal Microbiome Changes with the Breeding Season in a Wild Bird
    Escallón, C.; Belden, Lisa K.; Moore, Ignacio T. (Oxford University Press, 2018-09-29)
    The symbiotic microbial communities, or “microbiomes,” that reside on animals are dynamic, and can be affected by the behavior and physiology of the host. These communities provide many critical beneficial functions for their hosts, but they can also include potential pathogens. In birds, bacteria residing in the cloaca form a complex community, including both gut and sexually-transmitted bacteria. Transmission of cloacal bacteria among individuals is likely during the breeding season, when there is direct cloacal contact between individuals. In addition, the major energetic investment in reproduction can draw resources away from immune responses that might otherwise prevent the successful establishment of microbes. We assessed dynamic variation in the cloacal microbiome of free-living rufouscollared sparrows (Zonotrichia capensis) through sequential breeding and non-breeding seasons. We found that the cloacal bacterial communities differed between the sexes when they were in breeding condition. Further, in males, but not in females, the bacterial community became more diverse with the onset of reproduction, and then decreased in diversity as males transitioned to non-breeding condition. Individuals sampled across sequential breeding seasons did not accumulate more bacterial taxa over seasons, but bacterial community composition did change. Our results suggest that the cloacal microbiome in birds is dynamic and, especially in males, responsive to breeding condition.
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    Consequences of avian parental incubation behavior for within-clutch variance in incubation temperature and offspring behavioral phenotypes
    Hope, Sydney Frances (Virginia Tech, 2020-01-17)
    Parents can have large effects on their offspring by influencing the early developmental environment. In birds, a major way that parents can influence the early developmental environment is through egg incubation. Not only is incubation necessary for hatching success, but small changes of <1C in average incubation temperature have large effects on post-hatch offspring morphology and physiology. However, incubation is energetically costly and time-consuming for parents, and thus parents must allocate resources between incubation and self-maintenance. This can lead to differences in parental incubation behavior and egg temperatures among and within populations. Understanding which factors influence incubation, and the subsequent effects for offspring, is crucial for understanding parental effects, non-genetic drivers of phenotypic variation, and how environmental changes affect avian populations. I used wood ducks (Aix sponsa) as a study species to investigate how factors (disturbance, clutch size, ambient temperature) that influence parental demands may affect parental incubation behavior, physiology, and egg temperatures, and subsequently how egg temperatures affect offspring behavior and physiology. In a field experiment, I found that nest disturbance (i.e., capture) reduced both parent prolactin concentrations and the amount of time that parents spent incubating (Chapter 1). Further, ambient temperature was positively and clutch size negatively related to egg temperatures. Notably, in large clutches, differences in average incubation temperature among eggs within nests were large enough (i.e., >1C) to lead to different offspring phenotypes within broods (Chapter 2). Then, in a series of experiments in which I controlled incubation temperature, I provided evidence that lower average incubation temperatures lead to a reduced ability of ducklings to exit the nest cavity (Chapter 3), a more proactive behavioral phenotype (Chapter 4), a smaller body size, and a reduced efficiency in food consumption (Chapter 5), compared to those incubated at higher temperatures. Together, my dissertation illustrates how disturbances, clutch size, and ambient temperature can influence an important aspect of avian parental care, which has wide-ranging effects on offspring traits and fitness. This has broad implications for understanding the evolution of clutch size, development of behavior, and the effects of anthropogenic changes on wildlife.
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    Coping with Chronic Infection: The Role of Glucocorticoid Hormones in Mediating Resistance and Tolerance to Parasites
    Schoenle, Laura A. (Virginia Tech, 2017-07-10)
    Parasitic infections are ubiquitous, but the consequences to hosts can vary substantially. Variation in the consequences of infection can be related to individual differences in the use of two parasite defense strategies, resistance and tolerance. Resistance entails reducing parasite burden by removing parasites or restricting parasite reproduction. Tolerance involves minimizing the costs associated with a given parasite burden. Genetic variation, environmental conditions, and life history stage can contribute to variation in resistance and tolerance, but the physiological mechanisms that underlie investment in each strategy are not well understood. I proposed that glucocorticoid hormones, which mediate responses to challenges in the physical and social environment in vertebrates, might alter host investment in resistance and tolerance (Chapter I). Glucocorticoids influence a suite of physiological processes including immune function, resource allocation, and tissue growth, all which could alter resistance and tolerance. Using a combination of observational and experimental studies, I test the hypothesis that glucocorticoids mediate resistance and tolerance to infection in red-winged blackbirds (Agelaius phoeniceus) infected with Haemosporidians, including malaria (Plasmodium) and malaria-like (Haemoproteus and Leucocytozoon) parasites. I performed a medication experiment (Chapter II) to identify the physiological consequences of Haemosporidian infection and explored the relationships between glucocorticoids and parasite resistance and tolerance in both an observational field study and a hormone manipulation experiment (Chapters III and IV). Medication treatment effectively reduced Plasmodium burden, increased hematocrit and hemoglobin, and reduced the rate of red blood cell production (Chapter II). In an observational field study (Chapter III), red-winged blackbirds with higher plasma glucocorticoid concentrations maintained higher hematocrit than expected for their parasite burdens, suggesting a positive association between glucocorticoids and tolerance. In this study, I found no support for a relationship between glucocorticoids and resistance. However, experimental elevation of glucocorticoids (Chapter IV) yielded nearly opposite results: the higher of two doses of glucocorticoids increased Plasmodium burdens and caused a decrease in body mass with increasing parasite burden, indicative of a decrease in tolerance. I discuss possible causes of the differences in our observational and experimental studies and the implications of my work for future studies of individual variation in parasite tolerance (Chapter V).
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    Decentralized HVAC Operations: Novel Sensing Technologies and Control for Human-Aware HVAC Operations
    Jung, Wooyoung (Virginia Tech, 2020-04-13)
    Advances in Information and Communication Technology (ICT) paved the way for decentralized Heating, Ventilation, and Air-Conditioning (HVAC) HVAC operations. It has been envisioned that development of personal thermal comfort profiles leads to accurate predictions of each occupant's thermal comfort state and such information is employed in context-aware HVAC operations for energy efficiency. This dissertation has three key contributions in realizing this envisioned HVAC operation. First, it presents a systematic review of research trends and developments in context-aware HVAC operations. Second, it contributes to expanding the feasibility of the envisioned HVAC operation by introducing novel sensing technologies. Third, it contributes to shedding light on viability and potentials of comfort-aware operations (i.e., integrating personal thermal comfort models into HVAC control logic) through a comprehensive assessment of energy efficiency implications. In the first contribution, by developing a taxonomy, two major modalities – occupancy-driven and comfort-aware operations – in Human-In-The-Loop (HITL) HVAC operations were identified and reviewed quantitatively and qualitatively. The synthesis of previous studies has indicated that field evaluations of occupancy-driven operations showed lower potentials in energy saving, compared to the ones with comfort-aware operations. However, the results in comfort-aware operations could be biased given the small number of explorations. Moreover, required data representation schema have been presented to foster constructive performance assessments across different research efforts. In the end, the current state of research and future directions of HITL HVAC operations were discussed to shed light on future research need. As the second contribution, moving toward expanding the feasibility of comfort-aware operations, novel and smart sensing solutions have been introduced. It has been noted that, in order to have high accuracy in predicting individual's thermal comfort state (≥90%), user physiological response data play a key part. However, the limited number of applicable sensing technologies (e.g., infrared cameras) has impeded the potentials of implementation. After defining required characteristics in physiological sensing solutions in context of comfort-aware operations (applicability, sensitivity, ubiquity, and non-intrusiveness), the potentials of RGB cameras, Doppler radar sensors, and heat flux sensors were evaluated. RGB cameras, available in many smart computing devices, could be a ubiquitous solution in quantifying thermoregulation states. Leveraging the mechanism of skin blood perfusion, two thermoregulation state quantification methods have been developed. Then, applicability and sensitivity were checked with two experimental studies. In the first experimental study aiming to see applicability (distinguishing between 20 and 30C with fully acclimated human bodies), for 16 out of 18 human subjects, an increase in their blood perfusion was observed. In the second experimental study aiming to evaluate sensitivity (distinguishing responses to a continuous variation of air temperature from 20 to 30C), 10 out of 15 subjects showed a positive correlation between blood perfusion and thermal sensations. Also, the superiority of heat flux data, compared to skin temperature data, has been demonstrated in predicting personal thermal comfort states through the developments of machine-learning-based prediction models with feature engineering. Specifically, with random forest classifier, the median value of prediction accuracy was improved by 3.8%. Lastly, Doppler radar sensors were evaluated for their capability of quantifying user thermoregulation states leveraging the periodic movement of the chest/abdomen area induced by respiration. In an experimental study, the results showed that, with sufficient acclimation time, the DRS-based approach could show distinction between respiration states for two distinct air temperatures (20 and 30C). On the other hand, in a transient temperature without acclimation time, it was shown that, some of the human subjects (38.9%) used respiration as an active means of heat exchange for thermoregulation. Lastly, a comprehensive evaluation of comfort-aware operations' performance was carried out with a diverse set of contextual and operational factors. First, a novel comfort-aware operation strategy was introduced to leverage personal sensitivity to thermal comfort (i.e., different responses to temperature changes; e.g., sensitive to being cold) in optimization. By developing an agent-based simulation framework and thorough diverse scenarios with different numbers and combinations of occupants (i.e., human agents in the simulation), it was shown that this approach is superior in generating collectively satisfying environments against other approaches focusing on individual preferred temperatures in selection of optimized setpoints. The energy implications of comfort-aware operations were also evaluated to understand the impact from a wide range of factors (e.g., human and building factors) and their combinatorial effect given the uncertainty of multioccupancy scenarios. The results demonstrated that characteristics of occupants' thermal comfort profiles are dominant in impacting the energy use patterns, followed by the number of occupants, and the operational strategies. In addition, when it comes to energy efficiency, more occupants in a thermal zone/building result in reducing the efficacy of comfort-driven operation (i.e., the integration of personal thermal comfort profiles). Hence, this study provided a better understanding of true viability of comfort-driven HVAC operations and provided the probabilistic bounds of energy saving potentials. These series of studies have been presented as seven journal articles and they are included in this dissertation.
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    Dynamic Gap-Crossing Movements in Jumping and Flying Snakes
    Graham, Michelle Rebecca (Virginia Tech, 2022-05-23)
    Gap crossing is a regular locomotor activity for arboreal animals. The distance between branches likely plays a role in determining whether an animal is capable of crossing a given gap, and what locomotor behavior it uses to do so. Yet, despite the importance of gap distance as a physical parameter influencing gap crossing behavior, the precise relationships between gap distance and movement kinematics have been explored in only a very small number of species. One particularly interesting group of arboreal inhabitants are the flying snakes (Chrysopelea). This species is able to use a dynamic "J-loop" movement to launch its glides, but it is not known whether it is also capable of using such jumps to cross smaller gaps between tree branches. This dissertation addresses this knowledge gap, and investigates the influence of gap distance on crossing behavior and kinematics in three closely-related species of snake: Chrysopelea paradisi, a species of flying snake, and two species from the sister genus, Dendrelaphis, neither of which can glide. Chapter 2 is a literature review of the biomechanics of gap crossing, specifically focusing on the role played by gap distance, and establishes the context for the rest of the work. Chapter 3 presents a detailed study of how increasing gap size influences the behavior and kinematics of gap crossing in C. paradisi, showing that this species uses increasingly dynamic movements to cross gaps of increasing size. Chapter 4 explores the same relationships between gap size and kinematics in D. punctulatus and D. calligastra, revealing remarkable similarities between the three species, suggesting the possibility that dynamic gap crossing may have evolved prior to gliding in this clade. Finally, chapter 5 addresses the role played by gap distance in limiting the non-dynamic, cantilever movements used by these species to cross small gaps, comparing observed stopping distances to those predicted by various torque-related limitations.
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    Effects of ammonium perchlorate exposure on the thyroid function and the expression of thyroid-responsive genes in Japanese quail embryos and post hatch chicks
    Chen, Yu (Virginia Tech, 2008-04-28)
    Perchlorate ion interferes with thyroid function by competitively inhibiting the sodium-iodide symporter, thus blocking iodide uptake into the thyroid gland. In this study, the effect of perchlorate exposure on thyroid function and thyroid-responsive gene expression were examined in (1) embryos from eggs laid by perchlorate-treated Japanese quail hens and (2) perchlorate-treated young Japanese quail. I hypothesized that perchlorate exposure would decrease thyroid function and that the consequent hypothyroidism would alter the expression of thyroid sensitive genes. Laying Japanese quail hens were treated with 2000 mg/l and 4000 mg/l ammonium perchlorate in drinking water. Eggs from these hens were incubated. Embryos, exposed to perchlorate in the egg, were sacrificed at day 14 of the 16.5 day incubation period. Japanese quail chicks, 4-5 days old, were treated with 2000 mg/l ammonium perchlorate in drinking water for 2 and 7.5 weeks. Thyroid status was evaluated by measuring plasma thyroid hormone concentrations, thyroid gland weight and thyroidal thyroid hormone storage. Expression of thyroid-responsive genes was evaluated by measuring the mRNA levels of Type 2 deiodinase (D2) in the brain and liver, RC3/neurogranin mRNA level in the brain and Spot 14 mRNA level in the liver. Maternal perchlorate exposure led to embryonic hypothyroidism, demonstrated by thyroid hypertrophy and very low embryonic thyroidal TH storage. Embryonic hypothyroidism decreased body growth and increased D2 mRNA level in the liver (a presumed compensatory response to hypothyroidism) but did not affect the mRNA levels of D2 and RC3 in the brain. Spot 14 mRNA was not detected in embryonic liver. In the second part of the study, quail chicks showed early signs of hypothyroidism after two weeks of 2000 mg/l ammonium perchlorate exposure; plasma concentration and thyroid gland stores of both T4 and T3 were significantly decreased. After 7.5 weeks of perchlorate exposure, all thyroid variables measured indicated that the chicks had become overtly hypothyroid. D2 mRNA level was increased, a compensatory response to hypothyroidism, and spot 14 mRNA level was decreased, a substrate-driven response in the liver of quail chicks after two weeks of perchlorate exposure. However, no difference was observed in the mRNA levels of D2 and spot 14 in the liver after 7.5 weeks of perchlorate exposure, suggesting there was some adaptation to the hypothyroid condition. The mRNA level of D2 and RC3 in the brain was not affected by perchlorate-induced hypothyroidism in quail chicks after either 2 or 7.5 weeks of perchlorate exposure. As in the embryos, this suggests the brain of chicks was "protected" from the hypothyroid body conditions.
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    The effects of induced hypothyroidism on the glucocorticoid stress response in Japanese quail (Coturnix japonica)
    Weigel, Eric Roan (Virginia Tech, 2007-06-28)
    Many aspects of biological function are affected by hormones, from physiology to behavior, and the synthesis and release of hormones in vertebrates are regulated by the endocrine axes of control. A growing body of research shows that the mechanisms underlying the endocrine axes of control are complex and interconnected, with many hormones having multiple effects, and with many interactions between axes. In this study, I examined the effects of decreased thyroid function on the glucocorticoid stress response in Japanese quail, a potential interaction between the hypothalamic-pituitary thyroid (HPT) and hypothalamic-pituitary adrenal (HPA) axes of control. I used the thyroid inhibitor ammonium perchlorate (AP) for 2 weeks and 5 weeks to induce two states of decreased thyroid function: a thyroid challenged state, in which birds have depleted thyroidal T4 content, but still maintain euthyroid (normal) concentrations of plasma T4, and a hypothyroid state, in which birds have depleted thyroidal T4 content and decreased concentrations of plasma T4. Thyroid function was assessed by measuring plasma T4 concentrations, thyroidal T4 content, and thyroid gland mass. I took blood samples from birds both immediately prior to and immediately following a 30 minute confinement and agitation stressor to evaluate the effects of decreases in thyroid function on basal and stress-induced plasma corticosterone and plasma T4 concentrations. I found two key results: First, although baseline levels of plasma corticosterone were unchanged, the corticosterone stress response was significantly blunted in both the thyroid challenged and hypothyroid birds as compared to controls. This finding suggests that the HPT and HPA axes are functionally connected in birds, and other evidence suggests this connection is likely at the pituitary or hypothalamic level. Second, in hypothyroid birds, plasma T4 concentrations were elevated (into the euthyroid range) in response to the experimental stressor, although no change in plasma T4 was observed in thyroid challenged or control birds. This finding suggests that plasma T4 may have a permissive role in mounting a stress response.
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    The Effects of Limited Winter Food Availability on the Population Dynamics, Energy Reserves, and Feather Molt of the Swamp Sparrow
    Danner, Raymond Michael (Virginia Tech, 2012-06-15)
    Small birds likely face energetic challenges in temperate zone winters posed by cold weather coupled with food scarcity. These challenges are often assumed to occur, but are rarely experimentally tested. I hypothesized that the naturally occurring, lower abundances of food in temperate zone winters limit a bird's ability to acquire optimal energy and ultimately limit fitness. In this dissertation, I show that supplementation of food decreased mortality and improved traits potentially associated with future reproductive success of wild swamp sparrows (Melospiza georgiana), supporting the hypothesis that winter food abundance limits fitness. These results come from a replicated and controlled food supplementation experiment conducted over three years. First, I demonstrated that following food addition, immigration increased, leading to higher densities, and that all age/sex classes experienced higher survival and maintained larger energy reserves (Chapter I). Survival was positively related to energy reserves, indicating that food availability limits survival through a bird's ability to maintain sufficient fat. In addition to causing mortality in winter, food limitation of energy reserves may carry over to affect future reproductive success by influencing timing of preparations for breeding, including migration. In Chapter II, I show that swamp sparrows decreased fat reserves over each winter, despite unlimited food availability, indicating that they adaptively regulated fat reserves, potentially to balance starvation and depredation risks. Fat reserves of control birds tracked recent temperature and control birds lost muscle throughout the winter, indicating that they were limited by food and were unable to reach optimal fat levels on a daily basis. These results suggest that limitation of energy reserves by food availability can be influenced by temperature and predator abundance. Lastly, I demonstrated that food abundance limits the timing of molt in the wild (Chapter III), an unprecedented finding. Because molt, migration, and breeding typically do not overlap, early molt might lead to earlier migration and breeding. Therefore, we hypothesize that timing of molt is another mechanism by which winter food abundance can limit reproductive success. These results provide strong evidence that food availability can limit wintering temperate migrants in a variety of ways.
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    Effects of the antibiotic tetracycline on the honey bee gut microbiome
    Gregory, Casey L. (Virginia Tech, 2024-05-08)
    Host-associated microbial communities, also known as microbiomes, are essential to the health of their hosts, and disturbance of these communities can negatively impact host fitness. The honey bee gut microbiome is a relatively simple host-associated community that makes an excellent model system for studying microbiome stability. In addition, honey bees are essential agricultural pollinators, so factors that impact their health are important for food security. The presented research focused on the stability of the honey bee gut microbiome in response to disturbance from the antibiotic tetracycline. Tetracycline was chosen because it is the most commonly used antibiotic in beekeeping, and may have negative effects on bees through the disruption of their gut microbiomes. The first study presents a new fecal sampling method for studying the honey bee gut microbiome of individual bees over time. This method accurately represented bacterial community structure in the gut microbiome as determined with 16S rRNA gene amplicon sequencing, as fecal and whole gut samples did not differ significantly for individual bees. The fecal sampling technique was then used to examine changes to individual honey bee gut bacterial communities before and after tetracycline exposure. Minimal differences in gut community structure were detected prior to and five days after tetracycline treatment. However, there was variability in how individual gut microbiomes were affected by tetracycline treatment, highlighting the importance of intraspecific variation in response to disturbance. The second study investigated whether the timing of disturbance during a host's life impacts microbiome community stability. Newly emerged bees were treated with tetracycline, returned to their hive, and recollected 7 or 14 days later. The gut communities of the bees were then characterized using 16S rRNA gene amplicon sequencing. Gut microbiome structure of bees treated with tetracycline at emergence differed from controls both 7 and 14 days after emergence, with the antibiotic-treated bees having lower community richness overall. This study showed that early life disturbance of host-associated microbial communities can influence microbiome structure later in life. The final study describes the occurrence of antibiotic resistance genes (ARGs) in honey bee gut bacterial symbionts from hives across the US. Honey bee gut metagenomes were sampled from hives at 13 apiaries located in a transect from Virginia to Washington, and ARG presence was assessed across the sites. We also specifically quantified the abundances of two common tetracycline resistance genes (tet(B) and tet(M)) across apiaries. ARGs, both for antibiotics used in beekeeping and unrelated antibiotics, were detected in honey bee gut bacteria from all apiaries. Tetracycline resistance genes were the most common across all apiaries, and the abundance of two tetracycline resistance genes varied by apiary. Members of the honey bee gut microbiome contained different proportions of ARGs, but taxa within a single family contained similar proportions, possibly indicating phylogeny plays a role in ARG accumulation. In particular, Gilliamella and Frischella, both in the family Orbaceae, contained the highest percentages of ARGs. The results from this study suggest honey bee bacteria act as reservoirs of ARGs. Overall, the presented research contributes to the field of biology by highlighting the importance of intraspecific variation in host-associated microbial communities and presenting a new method for studying honey bee gut microbiome variation at the individual-level, showing that early life events in honey bees influence microbiome development, and suggesting that honey bee bacterial symbionts have adapted to deal with antibiotic disturbance through the accumulation of ARGs.