Browsing by Author "Voshell, J. Reese Jr."

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    Analyses of Two Aspects of Study Design for Bioassessment With Benthic Macroinvertebrates: Single Versus Multiple Habitat Sampling and Taxonomic Identification Level
    Hiner, Stephen W. (Virginia Tech, 2004-02-03)
    Bioassessment is the concept of evaluating the ecological condition of habitats by surveying the resident assemblages of living organisms. Conducting bioassessment with benthic macroinvertebrates is still evolving and continues to be refined. There are strongly divided opinions about study design, sampling methods, laboratory analyses, and data analysis. Two issues that are currently being debated about study design for bioassessment in streams were examined here: 1) what habitats within streams should be sampled; 2) and is it necessary to identify organisms to the species level? The influence of habitat sampling design and level of taxonomic identification on the interpretation of ecological conditions of ten small streams in western Virginia was examined. Cattle watering and grazing heavily affected five of these streams (impaired sites). The other five streams, with no recent cattle activity or other impact by man, were considered to be reference sites because they were minimally impaired and represented best attainable conditions. Inferential and non-inferential statistical analyses concluded that multiple habitat sampling design was more effective than a single habitat design (riffle only) at distinguishing impaired conditions, regardless of taxonomic level. It appeared that sampling design (riffle habitat versus multiple habitats) is more important than taxonomic identification level for distinguishing reference and impaired ecological conditions in this bioassessment study. All levels of taxonomic resolution, which were studied, showed that the macroinvertebrate assemblages at the reference and impaired sites were very different and the assemblages at the impaired sites were adversely affected by perturbation. This study supported the sampling of multiple habitats and identification to the family level as a design for best determining the ecological condition of streams in bioassessment.
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    Assessing the Nonpoint Source Pollutant Removal Efficiencies of a Two-Basin Stormwater Management System in an Urbanizing Watershed
    Lovern, Sharla Benjamin (Virginia Tech, 2000-05-15)
    Monitoring of a regional stormwater management facility, located on the Virginia Tech campus in Blacksburg VA, was conducted in order to assess its efficacy in reducing nonpoint source pollutant losses downstream. The facility design includes both an upper water quality (wet) pond and a lower 100-yr-event quantity (dry) pond. These on-stream ponds capture both baseflow and storm runoff from the southern portion of the Virginia Tech campus and surrounding lands, and release the water back to the unnamed stream shortly above its conjunction with Stroubles Creek, a tributary of the New River. Monitoring sites for flow measurement, water quality sampling, and biotic assessments (habitat evaluation and rapid bioassessment of benthic macroinvertebrates) were located above and below each of the ponds. Both grab samples and automated samples were collected at these stations. Between 1997 and 1999, water quality grab samples included 35 baseflow samples and 22 stormflow samples. The grab samples were analyzed for concentrations of total suspended solids (TSS), metals, bacteria, and nutrients as well as temperature, pH, dissolved oxygen, conductivity, total organic carbon (TOC), and chemical oxygen demand (COD). Automated flow-weighted sampling was initiated in February of 1999 and results are reported through the end of October 1999. Thirty-three storms in 1999 were monitored for flow and various water quality parameters (TSS, TOC, COD, and nutrients). Pollutant loads and pollutant removal estimates were calculated with regard to the wet pond, dry pond, and the combined facility. Two types of pollutant removal efficiencies were calculated: (1) the EMC efficiency, based on pollutant concentrations from individual storms; and (2) the SOL efficiency, based on pollutant loads, to estimate long-term performance over the study period. Benthic macroinvertebrate sampling and habitat assessment were performed in both 1997 and 1999. In addition, a preliminary investigation of pond characteristics was conducted, including measurements of water quality and composition, sediment deposition and composition, and residence time. As a system, the stormwater management facility appears to have minimum impact on improving the downstream water quality. Pollutant concentrations and loads both appear to increase downstream of the facility as compared to upstream, during both storm event and baseflow periods. Monitoring results of the benthic assemblages showed evidence of moderate to high impairment at all sampling locations, and habitat assessments showed evidence of high sedimentation levels within the stream, even after installation of the stormwater management facility. Total suspended solids (TSS) concentration removal efficiency was 10% for the combined wet pond and dry pond system, much lower than the 80 to 90% TSS removal expected for properly functioning stormwater management facilities (Hartigan, 1989). There is some evidence of sedimentation within the ponds because of a slight reduction in sediment-bound constituent export, but the dissolved nutrient constituents had either very low and most often negative (indicating pollutant export) removal efficiencies. Concentrations of metals measured in the stream often exceeded their respective acute and chronic water quality criteria at all sampling locations. Pollutant removal efficiencies measured in the wet pond are atypical of those reported in the literature (Schueler, 1993). Insufficient residence time (two days compared to the optimal two weeks), and wet pond embankment failure are likely the principal causes of the wet pond's inadequate performance and thus, the inadequate performance of the overall facility. TSS removal efficiencies were low in the wet pond (19% for concentrations and 33% for loads) compared to the 80 to 90% expected for similar ponds. Nevertheless, the wet pond reduced the concentrations of several pollutants typically associated with TSS and not likely to be associated with the fill material for the wet pond embankment. Zinc concentrations in sediment cores were highest near the pond inlet, where the majority of sedimentation occurs. During storm events, the following results were noted. Copper and zinc concentrations in 1998 were lower at the pond outlet as compared to the pond inlet, and TOC concentrations and loads were also reduced by the wet pond (13% for concentrations and 12% for loads). However, sedimentation is also expected to remove phosphorusl, and wet pond phosphorus loads were only reduced by 10% and 3% for orthophosphorus and total phosphorus, respectively. Because the wet pond is undersized with respect to the watershed it serves (surface area less than 1% of the watershed area (0.87 ha), as compared to the 3% ratio often recommended for optimal pollutant removal (Athanas, 1988)), higher removal efficiencies were found during baseflow periods. The greatest reductions in baseflow concentrations were for ammonia (67%), nitrate (57%), total nitrogen (54%), and COD (45%). However, the residence time of two days appears to be insufficient to reduce fecal coliform concentrations in the stream, and over 40% of the fecal coliform samples collected exceeded the water quality standard for contact recreation (DEQ-WQS, 1997). Furthermore, the wet pond did not appear to reduce TSS or TOC during baseflow periods. Export of TSS (-29% EMC efficiency) and TOC (-44% EMC efficiency) from the wet pond during baseflow periods is likely due to the wet pond embankment failure as well as pond eutrophication. Eutrophication processes are favored by the water temperature increase as flow passes through the shallow wet pond. The wet pond increased downstream temperatures by approximately 8°C above inflow temperatures during the summer, and to levels above 21°C which cannot be tolerated by sensitive coldwater species (Schueler, 1987). The dry pond did not remove dissolved nutrient constituents or other pollutants during baseflow periods, but there is some evidence of sedimentation within the dry pond during storm events. During storm events, the dry pond was effective in removing TSS, with a concentration removal efficiency of 69% (EMC efficiency) and loading removal efficiency of 43% (SOL Efficiency). Removal of TKN and total phosphorus (36% and 37% respectively for concentrations) within the dry pond is further evidence of sedimentation within the dry pond. The wet pond embankment was built in 1997, and monitoring occurred during a potential stabilization period when evidence of water quality benefits are slow to appear, especially with respect to downstream habitat and aquatic communities. Some benefits which could have been observed more immediately may have been negated or masked by the progressive erosion of the wet pond embankment as a result of a design flaw. Further complicating the results is the appearance; based on observations of extended drawdown time and results from a water budget analysis in the wet pond (where inflow substantially exceeds inflow); that groundwater interacts with the pond in a complicated fashion, possibly including both recharge and discharge. To fully understand the impact of the stormwater management facility on the water quantity and quality within this tributary of Stroubles Creek, monitoring efforts should continue after the wet pond embankment is repaired and is fully operational. If biotic community improvement is desired, the stabilization period could be defined by the time necessary to flush out accumulated sediment within the channel. Monitoring efforts should also expand to include the investigation of the groundwater regime and water level fluctuations within the wet pond. Further measurements of pollutant removal processes and influences upon those processes within the wet pond should also be considered. Last, the influence of the stormwater management facility on downstream flow regimes should be investigated to assess the adequacy of its performance with regard to flow control and prevention of stream channel degradation.
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    Benthic Macroinvertebrate Susceptibility to Trout Farm Effluents
    Roberts, Lenn Darrell (Virginia Tech, 2005-08-30)
    The direct effects of a Virginia trout farm on benthic macroinvertebrates were examined using multiple approaches. Static laboratory tests with the amphipod, Hyallela azteca, were conducted with exposures to water taken from a spring, effluent above a sedimentation basin, and effluent below a sedimentation basin. On-site mesocosms were constructed to expose previously colonized artificial substrates to the same treatments as the laboratory tests. Flat-headed mayflies were also collected from a nearby stream and transported to the mesocosms for a 10 day exposure. There was no significant difference between treatments in the laboratory tests after 20 days, but after 28 days the control was significantly lower than the above sedimentation basin treatment in one test. In the multispecies field tests, a clear decrease in total invertebrate abundance and EPT abundance was seen in the effluent treatments compared to the spring water treatments, with a slight improvement in survival in the treatment below the sedimentation basin. However, only total invertebrate abundance after 21 days produced statistically significant differences. A significant difference was detected between the effluent and the spring treatments in the flat-headed mayfly field test. We suggest that the effects seen in this study do not explain the lack of taxa richness in the receiving stream. The main cause of mortality from trout effluents appears to be solids accumulating upon the organisms, and sedimentation basins should be effective best management practices for protecting macroinvertebrates.
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    Biology of immature Culicoides variipennis ssp. australis (Coq.) (Diptera:Ceratopogonidae) at Saltville, VA
    Vaughan, Jefferson Archer (Virginia Polytechnic Institute and State University, 1985)
    The larval and pupal biology of a unique population of gulicoides variipennis inhabiting the brine ponds of Saltville, VA was studied. Developmental threshold temperatures (OC) and thermal constants (Odays) for larvae and pupae were 9.6OC and 387Odays (larval stage) and 9.6OC and 3OOdays (pupal stage) respectively. Accumulated heat units recorded in the field ranged from 366—376Odays between successive generations in the summer. Heat accumulations required for completion of immature development of Q. variipennis were found to be much greater (83lOdays) for the overwintering generation. During the summer, larval/pupal distribution within the littoral zone of a brine pond was confined to the surface cm of mud at or near the shoreline. Insects overwintered farther offshore, mostly as 3rd instars. In ear1y' March, most larvae had xnolted to 4th instars and migrated above shoreline to pupate. Adult emergence occurred in April. Three summer generations were documented for 1983-1984 at Saltville._ Life tables and survivorship curves were calculated for the overwintering generation and the first summer generations for 1983 and 1984. For the overwintering generation, there was a relatively constant mortality rate between successive ageclasses (Type II survivorship curve). During the summer, there was relatively little mortality between successive larval age—classes but a dramatic increase in mortality was evident at the pupal stage (Type I survivorship curve). Late instar larvae were found to migrate from the shoreline onto the exposed mudflats to pupate, thus becoming vulnerable to predation by ants and carabid beetles. Excellent survival rates of the larvae during the summer was attributed to habitat stability, the paucity of predators and parasites and abundant microfloral content (i.e. food} of the pond water. Intra-specific competition for food resources appeared to be alleviated somewhat by partitioning of those resources on a diurnal cycle.
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    Biotic and abiotic responses to rural development and legacy agriculture by southern Appalachain streams
    Burcher, Chris L. (Virginia Tech, 2005-04-12)
    Streams are integrative systems spanning multiple spatial and temporal scales. Stream researchers, land-use managers, and policy decision makers must consider the downstream displacement of streams when approaching questions about stream ecosystems. The study of how anthropogenic land-use influences streams demands an ecosystem perspective, and this dissertation is an example of applying large scale analyses of stream reach responses, and linking the activity of humans in the landscape to stream structure and function. I investigate whether rural development and agriculture land-cover types influence abiotic and biotic stream responses. I establish a method for considering land-cover as an independent variable at multiple scales throughout a streams' watershed using hydraulic modeling. The travel time required for water to drain from the watershed to a stream reach provided a continuous index to delimit watershed sub portions along a spatial continuum. Within travel time zones (TTZs), I consider land-use at increasingly larger scales relative to a stream reach within which biotic responses are typically measured. By partitioning land-cover in TTZs, I was able to determine the spatial scale at which land-cover was most likely to influence in-stream responses. I quantified a suite of physical and biotic responses typical to the aquatic ecology literature, and found that streams did not respond much to rural development. Rural development influenced suspended and depositional sediments, and likely altered watershed hydrology though I was unable to find significant evidence supporting a hydrologic effect. Subtle differences in assemblages suggest that differences in sediment dynamics influenced macroinvertebrates and fish. Using the Land Cover Cascade (LCC) design, I link the influence of land-cover to biotic responses through a suite of multivariate models, focusing on sediment dynamics in an attempt to capture the subtle influence of hydrology and sediment dynamics. My dissertation provides future researchers with improved methods for considering land-cover as an independent variable, as well as introduces multivariate models that link land-cover to sediment dynamics and biota. My dissertation will assist future research projects in identifying specific mechanisms associated with stream responses to disturbance.
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    Cellulolytic responses to heavy metal accumulation in Corbicula fluminea and Mudalia dilatata
    Farris, Jerry L. (Virginia Tech, 1986-03-05)
    Cellulolytic responses of the Asiatic clam, Corbicula fluminea and a snail, Mudalia dilatata, to selected constituents of power plant effluents (i.e., zinc, cadmium, acidic and alkaline pH, individually and paired) were investigated in 30-day exposures. Exposures were conducted in both laboratory and field-oriented artificial streams and then validated in the river receiving system of a power plant. Cellulolytic activity was reduced by laboratory and field exposures to cadmium and zinc at all levels tested from 0.012 to 0.10 mg cadmium/L and generally at 0.025 to 1.0 mg zinc/L. Clams detected acute lethal levels of metal and used valve closure as an avoidance mechanism for 14 days. Snails, however, did not effectively avoid exposures and were more sensitive to acute stress during all exposures. These behavioral responses were corroborated by both cellulolytic activity and metal accumulation. Measurements of cellulolytic activity for both test species in laboratory exposures differed from those in field artificial streams. Reduced enzyme activity in controls by day 30 was attributed to artificially induced stress associated with the laboratory environment. This factor precluded any analysis of laboratory responses for periods of exposure longer than 20 days as well as recovery analysis. Field oriented artificial streams provided a sufficient environment to adequately assess long-term stress and recovery as measured by cellulolytic activity and metal accumulation in both clams and snails. Enzyme activity responded to metal exposure with respect to both degree and duration of exposure. Cadmium and zinc combined exposures caused significantly reduced cellulolytic activity at the same concentration as those for cadmium alone. Reduced enzyme activity caused by cadmium and zinc addition at levels that were not detectable suggested that the cellulolytic index was sensitive to sublethal stressors. This was supported by metal uptake patterns in clams and snails. Cellulolytic activity responded to zinc addition at alkaline and acidic pH in a manner that supported pH optima for cellulases and bioavailability of metals. Effects seen in macroinvertebrate assemblages (diversity, richness, and similarity) were compared with cellulolytic activity of caged Corbicula from a site specific power plant discharge. Enzyme activity inhibition was the most sensitive indicator measured. Reductions in cellulolytic activity at stations monitored for total zine content were consistent with effects seen at comparable exposures to zine in field-located artificial streams. A zine concentration of 0.05 mg/L consistently caused the first significant reductions in cellulolytic activity. This concentration is comparable to the U.S. Environmental Protection Agency's Water Quality Criteria value (0.047 mg/L zinc) for protection of aquatic life.
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    Characterization of pond effluents and biological and physicochemical assessment of receiving waters in Ghana
    Ansah, Yaw Boamah (Virginia Tech, 2010-04-09)
    This study was carried out to characterize ponds and aquaculture systems, and also to determine both the potential and actual impacts of pond aquaculture effluents on receiving stream quality in the Ashanti and Brong Ahafo regions of Ghana. Water, fish and macroinvertebrate samples were collected from upstream, downstream and nearby reference streams of, and questionnaires administered to, 32 farms. Total settleable solids were higher in ponds than reference streams (p = 0.0166); suspended solids was higher in ponds than reference streams (p = 0.0159) and upstream (p = 0.0361); and total phosphorus was higher in ponds than reference (p = 0.0274) and upstream (p = 0.0269). Total nitrogen was most clearly higher in ponds than all other locations: p = 0.0016, 0.0086 and 0.0154 for the differences between ponds and reference, upstream, and downstream respectively. BOD5 level was also higher in ponds than all locations (p = 0.0048, 0.0009, and 0.0012 respectively). Also, non-guarding fish species were more abundant in reference streams than downstream (p = 0.0214) and upstream (p = 0.0251), and sand-detritus spawning fish were less predominant in reference streams than upstream (p = 0.0222) and marginally less in downstream locations (p = 0.0539). A possible subsidy-stress response within study streams was also observed. Hence, ponds are potential sources of these water quality variables to receiving streams. Effluent-receiving streams, generally, were not much different from reference streams in terms of most the metrics of community structure and function used in the comparisons. Hence, even though receiving streams in Central Ghana may not be severely impacted by aquaculture effluents at the moment, the management of pond effluents will determine the scale of future impact. Vegetable, cereal, and livestock farming could serve as additional sources of fecal streptococci and coliform bacteria and nutrient-enrichment within the study area, besides aquaculture, and so these industries must also be included in efforts to minimize pollution of these streams.
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    Community ecology of aquatic insects in forested headwater streams in the southern Appalachians
    Sokol, Eric R. (Virginia Tech, 2009-09-04)
    Competing paradigms of community assembly emphasize different mechanisms for predicting patterns in biogeography. Niche assembly emphasizes the role of environmental gradients as filters that organize a metacommunity by locally selecting colonizers with similar functional traits, whereas dispersal assembly emphasizes the importance of source pool characteristics and dispersal limitation in organizing a metacommunity. In this study, I developed a framework that uses spatially explicit patterns in taxonomic and functional measures of community composition as diagnostics for community assembly processes for benthic macroinvertebrates in headwater streams in the southern Appalachians. Distance decay in taxonomic and functional similarity was used to determine the scales at which taxonomic turnover occurred within functional niches. Trait-neutral models of community composition were used as null models to assess which functional traits were the best candidates to explain how community composition was influenced by environmental gradients: an assessment of niche-based community assembly. Regional scale patterns suggested that niche-based community assembly was the dominant mechanism organizing community composition in headwater streams at local scales (<30km). Therefore, I compared how well trait-neutral models identified functional traits as relevant to community sorting against how well observed trait distributions correlated with environmental variation at a local scale, in the Ray Branch catchment (<10km study extent). Functional traits exhibiting non-random distributions within the Ray Branch watershed were most strongly correlated with environmental variation. Lastly, I assessed how the influences of niche and dispersal assembly on benthic macroinvertebrate community composition were affected by disturbance (shelterwood logging). Environmental variables defining the habitat template, and macroinvertebrate community composition, were measured before and after the disturbance; and path analysis was used to quantify the disturbance effect. The relationship between environmental variation and functional composition increased following logging, indicating disturbance augmented the influence of environmental filters, and consequently, the importance of niche-based community assembly. My dissertation provides the framework for a novel assessment of taxonomic and functional community composition data to infer the types of ecological dynamics that organize communities in the landscape. Additionally, this work provides a theoretical basis for assessing how dominant ecological processes change, in predictable ways, in response to changes in the habitat template.
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    A contribution to the knowledge of the odonata
    Carle, Frank Louis (Virginia Tech, 1982-06-16)
    Theories concerning the origin of insect wings and flight are reviewed and a new scenario for their origin proposed. It is suggested that environmental conditions of the small stream were responsible for the evolution of insect flight, and that thermoregulation as well as respiration was important in the preadaptation of wings. The possibility that the five paired convex-concave vein systems each represented a dorsal-ventral blood channel is suggested. Odonate wing vein homology and nomenclatural systems, and phylogeny are reviewed. The process of vein loss is evaluated in the Palaeoptera and a new system of odonate wing vein homologies proposed. The odonate wing mechanism is analyzed and the heretofore overlooked discal nodus characterized. Reevaluation of the comparative morphology of fossil and recent Odonata indicates that Protozygoptera and Protanisoptera represent evolutionary side branches, that the Anisozygoptera is polyphyletic, and that Isophlebiidae and Calopterygoidea are the most generalized Odonata known. Previous scenarios explaining evolution of the unique odonate copulatory process are reviewed. Considering the copulatory behavior of the Calopterygoidea generalized supports evolutionary trends toward male domination and in-flight completion of the process. Assuming direct sperm transfer the original odonate mode requires that originally oviposition be in tandem and that sperm transfer to and from male anterior abdominal sterna be accidental. In contrast, assuming an original indirect transfer of sperm leads to a copulatory sequence similar to that of the Odonata. The proposed scenario differs from others in that extraordinary postures are not envisioned, the process is completed at rest, and the odonate tandem hold is developed prior to copulation. Anisopteran morphology and phylogeny are reviewed and reliable dentification keys developed for North American families and genera, and for 180 anisopteran species collected in Virginia and vicinity. Each species is described and photographed, including seven new species. The biogeography of Virginia Anisoptera is best explained by overlapping biotic regions, the fauna being a mixture of eastern North American, boreal, and tropical elements. New efficient methods for collecting, preserving, and rearing Odonata are described.
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    Contributions to the autecology and ecosystematics of immature ceratopogonidae (Diptera), with emphasis on the tribes heteromyiini and sphaeromiini in the middle atlantic United States
    Knausenberger, Walter Ingolf (Virginia Tech, 1986-12-05)
    Biological and systematic study of the immature stages of the biting midges (Diptera: Ceratopogonidae) has been infrequent, although the family is one of the largest in the order, with over 4,000 species worldwide, and is ubiquitous among aquatic/semiaquatic environments. An ecosystematic analysis is presented here of the Ceratopogonidae in Virginia and contiguous states based mainly on a diversified biological field survey, with particular attention to associating immature stages with adults, and acquisition of ecological data. I emphasized (a) extraction of larvae and pupae; (b) two methods of rearing, developed for this study: individual rearing(IR) with an agar-nematode technique, and collective rearing from the habitat substrate in rearing cartons(RC); and (c) field trapping of adults at the habitats. In Section I, a faunistic assessment - the first of its sort - is provided of the total Virginia-region ceratopogonid fauna, in all developmental stages, with a focus on Culicoides, including a comparative evaluation of the relative abundance of the ceratopogonid genera with respect to methods of collecting and extracting. High taxonomic richness of the ceratopogonid fauna in the Middle Atlantic states is reflected in the 150 described and 42 undescribed (new) species recovered. A detailed geographic checklist for the 222 species of Virginia and the five contiguous states is presented and interpreted. At least 54 Culicoides species are present in the Middle Atlantic u.s. About half of the larval species can be determined in Virginia. With the results of this study, 93% of breeding sites for the genus are known in Virginia, more than for any other state. Section II assembles and synopsizes data on the autecology, life history, habitat, morphology, systematics, as well as geographic and seasonal occurrence of 28 species (3 new) and 10 genera in two related tribes of predaceous biting midges, Heteromyiini and Sphaeromiini (subfamily Ceratopogoninae). Shorelines are the "archetypical arena" in which these larvae thrive, typically above and below the water line. Their habitats and substrates are characterized in detail. The greatest diversity of larvae in these tribes consistently occurs in and along mid-reach streams{Order: 3 to 5). Mechanisms of ecological partitioning by sympatric species, and the differential adaptive significance of larval, pupal and adult morphological characters are evaluated. Taxonomic and ecological diversity in these groups are clearly related.
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    Development and Implementation of Integrative Bioassessment Techniques to Delineate Small Order Acid Mine Drainage Impacted Streams of the North Fork Powell River, Southwestern Virginia
    Schmidt, Travis Scott (Virginia Tech, 2001-09-10)
    Acid mine drainage (AMD) results from the oxidation of pyretic mineralogy, exposed by mining operations to oxygen and water. This reaction produces sulfuric acid and liberates heavy metals from the surrounding mineralogy and impairs water quality and freshwater communities. The U.S. Army Corps of Engineers has begun an ecosystem restoration project to remediate the abandoned mine land (AML) impacts to the North Fork Powell River (NFP) and is utilizing the ecotoxicological rating (ETR) system to delineate these affects to focus restoration efforts. The ETR was developed to summarize the integrative data into a single number ranging from 0 to 100, which is descriptive of the environmental integrity of a sampling station. The ETR is conceptualized to work as an academic grading scale (0 through 100), rating reference stations with A's (90-100) and B's (80-89) and impacted stations with C's (70-80), D's (60-70) and failures (F = 60). Two rounds of ETR investigations have evaluated seven headwater tributaries to the NFP including investigations of Ely and Puckett's Creek from 1997 and 1998. This thesis contains the results of the second series of ETR investigations at 41 stations in Cox Creek, Jone's Creek, Reed's Creek, Summers Fork, Straight Creek, and areas in the NFP. Eight stations were recommended for reclamation; CC 03, JCRF2 02, JCRF2 01, RCPS 09B, RCPS 11B, SULF 01, SU 02, and SU 01. Summers Fork was the most severely impacted watershed of the second round of ETR investigations. An effort to streamline the ETR to the most ecologically predictive parameters was successful in creating a system more time and cost efficient then the initial ETRs and exclusive of benthic macroinvertebrate surveys. The Modified ETR streamlined the ETR to just 5 parameters including; mean conductivity, mean Asian clam survival, mean aluminum (Al) and manganese (Mn) in the water column, and mean habitat score to describe the AMD impacts to small headwater streams. Also, an investigation was conducted to determine the mode of toxicity, (i.e., exposures to metal contaminated surface waters or sediments) by which Al and iron (Fe) dominated AMD impairs benthic macroinvertebrate communities. It was found that water column exposures both within and beyond the zone of pH depression are the most likely mode by which AMD impairs the benthic macroinvertebrate communities of the NFP.
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    The diet and growth of a leaf-shredding caddisfly, Pycnopsyche, in streams of contrasting disturbance histories
    Hutchens, John Jehu (Virginia Tech, 1994-06-28)
    This study investigated the diet and growth of a leaf-eating caddisfly, Pycnopsyche, in streams draining a >60 year-old reference forest and a 16-year-old c1earcut (disturbed) forest at Coweeta Hydrologic Laboratory in southwestern North Carolina. The objective was to examine whether Pycnopsyche larvae grew better on fast-decaying leaf types more prevalent in the disturbed streams (e.g., black birch) because leaf-eating insects (shredders) in a previous study were more productive in these streams despite having less food available. Larvae consumed mostly unidentified plant material in streams of both forest types over three seasons (fall, winter, and spring) which suggested larvae did not consume higher quality foods (e.g., algae) in disturbed streams. When fed 2-mo "conditioned" black birch and white oak leaves, lab experiment larvae grew significantly faster on birch leaves. However, when larvae were fed the same leaf types after 3 mo of conditioning, larvae grew significantly faster on oak leaves. A field growth experiment conducted for 42 d using leaf diets representative of both forest types and conditioned for 2 mo found Pycnopsyche grew better on the diet representative of the reference forest. found Pycnopsyche grew better on the diet representative of the reference forest.
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    Ecological studies of benthic macroinvertebrates for determining sedimentation impacts in Chattahoochee National Forest streams
    Longing, Scott Douglas (Virginia Tech, 2006-04-07)
    Understanding sedimentation impacts to benthic macroinvertebrates in headwater, mountain streams is a top priority of watershed management programs in the Chattahoochee National Forest. Five studies involving the analysis of historical, biological survey data and current data were conducted to improve our understanding of macroinvertebrate response to sedimentation and to support the development of biological information for sediment load models to be applied in the Chattooga River watershed. An initial analysis of historical data involving a composited, macroinvertebrate reach-scale sample revealed weak relationships between assemblage metrics and sedimentation, which was similar to results of two recent macroinvertebrate studies that found biological ratings of good or excellent with reported physical impact attributed to sedimentation. Those findings and field reconnaissance in the Chattooga River watershed revealed that patchy, coarse sands may be the primary issue of concern regarding sedimentation impact to benthic macroinvertebrates. Therefore, a modified sampling approach was used to investigate relationships of macroinvertebrates and environmental conditions that included micro-habitat patches containing coarse sands, a product of erosion associated with Southern Blue Ridge, silicate parent geology. At the microhabitat, patch scale, flow velocity was the main environmental factor associated with a macroinvertebrate assemblage gradient, and was significantly correlated with percent deposited sediment across 264 samples. The high dominance of just a few macroinvertebrate genera, and the majority lack of individual macroinvertebrate associations with dominant substrate types may suggest that the dominant macroinvertebrates utilize a multi-microhabitat portion of the streambed at any given time, which may be due to the homogenization of streambeds due to sand (providing ease of movement) and its immobility (low bedload volume and sand patch shift). Because flow was the only significantly correlated environmental variable on an assemblage gradient produced by ordination (and was individually correlated with dominant substrate and percent deposited sediment), a subsequent study was conducted to determine macroinvertebrate sensitivity to deposited sediments among two flow-differentiated habitat types. Results showed that more taxa were related to a gradient of percent deposited sediment in fast water habitats, and no taxa were positively correlated with percent deposited sediment. Indicator species analysis found a number of taxa that were associated with a four-level grouping of percent deposited sediment levels. Therefore, a final study involved calculating deposited sediment tolerance values using indicator species associations and individual cumulative abundances across percent deposited sediment levels. The final index developed from cumulative abundances showed a relationship with deposited sediment within the range 0 – 30%, and that low range was due to the low deposited sediment levels at which all 50% cumulative abundances fell (1 - 10%). The sedimentation index produced from indicator species analysis produced a reach-scale index that was related to percent pool embeddedness. Key findings from these studies are: (1) sand is the primary deposited sediment type, with most streambed comprised of cobble-sand substrate, (2) few taxa are associated with deposited sand substrate, (3) there are high numbers of a relatively few dominant taxa across samples and streams, (4) macroinvertebrate response to deposited sediments is greatest in fast water habitats, and (5) the developed sedimentation biotic index is a potential, assemblage-level indicator of increasing sedimentation in these headwater systems. The functional and habit organization of the four most dominant taxa determined in recent studies suggest that they may be utilizing sand patches for crawling and collecting food, therefore structurally adapting to long-term, press disturbances due to historical and contemporary anthropogenic activities and natural erosion. In addition, macroinvertebrate assemblage composition in these streams indicates overall good "health" and suggests streambed stability in the presence of a large portion of coarse sand. However, an important question that remains involves sand movement along streambeds and the ecological consequences of continued sediment inputs to these headwater systems.
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    Ecology of benthic macroinvertebrates in experimental ponds
    Christman, Van D. (Virginia Tech, 1991-09-07)
    I studied life history parameters of 5 taxa of aquatic insects in the orders Ephemeroptera and Odonata, successional patterns over 2 years of pond development, and precision of 15 biological metrics in a series of 6 replicate experimental ponds from March 1989 to April 1990. I determined voltinism, emergence patterns, larval growth rates and annual production for Caenis amica (Ephemeroptera: Caenidae), Callibaetis floridanus (Ephemeroptera: Baetidae), Anax junius (Odonata: Aeshnidae), Gomphus exilis (Odonata: Gomphidae), and Enallagma civile (Odonata: Coenagrionidae). Growth rates ranged from 0.011 to 0.025 mg DW/d for Ephemeroptera and from 0.012 to 0.061 mg DW/d for Odonata. Annual production ranged from 5 to 11 mg DW/sampler/yr for Ephemeroptera and from 10 to 673 mg DW/sampler/yr for Odonata. Comparison of the benthic macroinvertebrate community at the end of year 1 to the benthic macroinvertebrate community at the end of year 2 showed no significant differences for community summary measures (total density, taxa richness, diversity, Bray-Curtis similarity index); however, some individual taxa densities were significantly lower at the end of year 2. Physicochemical parameters measured indicated that the ponds were oligotrophic. Submerged macrophytes colonized and became established in most of the ponds during year 2. A few noninsect taxa were not present in expected numbers, probably due to lack of efficient dispersal mechanisms. Fifteen metrics were analyzed by a statistical procedure that indicates the percent change that must occur (detection limit) to detect true differences between two means. The metrics with the lowest detection limits (usually < 20%) were taxa richness, EOT index (number of taxa in the orders Ephemeroptera, Odonata, and Trichoptera), proportion of Chironominae/Orthocladiinae, and proportion of collector-gatherers. Detection limits of < 20% on all dates were also obtained for taxa richness and EOT index metrics when analyzed using dip net samples. Density metrics only allowed detection limits of about 50% on most dates. This study provided needed information on the life history of taxa important in shallow, lentic ecosystems, ecological succession in newly created ponds, and statistically sound and ecologically meaningful metrics. This study also provides a valuable baseline for impact assessment work in experimental ponds.
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    An Ecotoxicological Assessment of Upper Clinch River Tributaries, Virginia
    Locke, Branden Alyssa (Virginia Tech, 2005-04-14)
    The Clinch River, Virginia is known for high aquatic biodiversity, particularly Unionidae which are declining at remarkable rates. Studies conducted on the mainstem have only addressed effects of point-source stressors (various toxic spills and effluents from the Clinch River Plant (CRP), Carbo, Virginia) that have been introduced into the Clinch River. It is hypothesized that the tributaries of the Clinch River deliver a variety of stressors to the mainstem, which may affect the diverse fauna. The aquatic health of 19 upper Clinch River tributaries, Virginia, was assessed via ecotoxicological ratings that indicated the least healthy catchments were associated with mining activity (Dumps, Russell and Coal Creeks). Tributaries were categorized by land use and mining streams were significantly different from agricultural and forested streams (F = 9.63, p<0.0001). Tributaries with ecotoxicological ratings (ETR) <80 from 100 were deemed suboptimal and thus studied further. Using identical response variables and upstream and downstream sites, resulting ETRs for nine streams indicated no model significance regarding land use, year, or site. Variability within treatments and low sampling sizes contributed to lack of significance, and results indicate that future studies need to be designed incorporating sites with analogous land use stressors. This first assessment of upper Clinch River tributaries indicates the catchments requiring remediation are Dumps, Russell and Coal Creeks, while tributaries requiring extensive evaluations are Big, Lick, Swords, Big Spring, Guest River, Cavitts and Middle Creeks. Tributaries that were deemed healthy (ETRs >80) were Big Stony, Copper, Indian, Stock, Little River and Cove Creeks.
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    The effectiveness of using volunteers for biological monitoring of streams
    Engel, Sarah Rose (Virginia Tech, 2000-12-15)
    An increase in public environmental awareness and a decrease in resources within government regulatory agencies have led to a larger interest in volunteer biomonitoring programs. Government agencies are currently using volunteer data for official purposes with increasing frequency, but questions have been raised about the validity of the data collected by volunteers who have only limited training and experience. Therefore, we conducted a detailed study to assess, modify, and validate the Virginia Save-Our-Streams (SOS) program, which is a volunteer organization collecting macroinvertebrate data. Sites were sampled using professional methods concurrently with volunteers who utilized the SOS protocol. The volunteer samples were retained for further laboratory analysis. In addition, numerous sites previously sampled by volunteers were re-sampled using professional methods. The data were statistically analyzed to determine if the results of volunteers and professional aquatic biologists were correlated and if they arrived at the same conclusions about ecological condition. It was determined that the Virginia SOS method, and probably other similar volunteer methods, consistently overrate ecological condition. This means that streams impaired by pollution could go unreported, if they are monitored exclusively by volunteers. The cause of this overestimation was determined to be the overly simplistic SOS metric, which is based solely on the presence or absence of taxa. The SOS protocol for data analysis was made more quantitative by developing a multimetric index that is appropriate for use by volunteers. The SOS sampling protocol was modified slightly to obtain actual counts of the different kinds of macroinvertebrates, which allowed for calculation of metrics. Sorting effort and taxonomic level of identification were not changed so that currently participating volunteers would not be excluded because of the need for expensive equipment or advanced technical training. The modified SOS protocol was evaluated by a different set of concurrent samples taken by volunteers and professionals, but using the same statistical techniques. The modified SOS protocol proved to be feasible for volunteers. The new SOS multimetric index correlated well with a professional multimetric index. The conclusions about ecological condition derived from the volunteer multimetric index agreed very closely with those made by professional aquatic biologists. This study demonstrated that volunteer biomonitoring programs can provide reliable data, but every volunteer program needs to be thoroughly validated by statistical comparisons to the professional methods being used in that area.
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    The Effects of Climate Change and Long-term Fire Suppression on Ephemeral Pond Communities in the Southeastern United States
    Chandler, Houston Cawthorn (Virginia Tech, 2015-01-15)
    In the southeastern United States, ephemeral wetlands in pine flatwoods provide important habitat for amphibians and aquatic invertebrates, but extensive deforestation has destroyed or isolated many wetlands and fire suppression has altered vegetation in others. My goals were to identify how wetland hydroperiods have changed through time and to examine the effects of long-term fire suppression on aquatic communities, including Reticulated Flatwoods Salamanders (Ambystoma bishopi) and Ornate Chorus Frogs (Pseudacris ornata). Chapter 1 used a modeling approach to relate wetland hydroperiods to current climate conditions and to hindcast historic conditions. Over the past 118 years, hydroperiods were often unfavorable for A. bishopi reproduction, and in recent years hydroperiods were shortened by persistent drought. Chapters 2 and 3 focused on identifying the effects of shifting from an open, grass dominated wetland to a wetland with high canopy cover and little herbaceous vegetation. In Chapter 2, I quantified amphibian and invertebrate communities in several wetlands. A. bishopi and P. ornata tended to occupy wetlands with lower canopy cover and higher herbaceous vegetation cover. Aquatic invertebrate abundance was generally higher in wetlands with lower shrub density and lower canopy cover. In Chapter 3, I examined how a reduction in herbaceous vegetation affected tadpoles when a predatory crayfish was present using two experiments. Crayfish were effective predators of both species across all vegetation treatments and often caused nonlethal tail injury. My results suggest that managers should focus on ensuring that wetland basins regularly burn, and wetlands with longer hydroperiods should be a management priority.
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    Effects of stream network topology on fish assemblage structure and bioassessment sensitivity in the mid-Atlantic highlands, USA
    Hitt, Nathaniel Patterson (Virginia Tech, 2007-04-19)
    Stream fish assemblages exist within stream networks defined by the size and proximity of connected streams (i.e., stream network topology). The spatial position of sites within stream networks may therefore regulate opportunities for fish dispersal to access distant resources or colonize "new" habitats. Such inter-stream dispersal dynamics will influence local fish assemblage structure and the vulnerability of local assemblages to anthropogenic stressors. In this dissertation, I explored the effects of stream network topology on fish assemblage structure in the mid-Atlantic highlands, USA and tested the hypothesis that dispersal would affect the sensitivity of fish-based environmental quality assessments (i.e., bioassessments). In chapter 1, I evaluated the effects of stream networks by comparing fish assemblages between sites with and without large downstream confluences (>3rd order) in western Virginia, USA (i.e., mainstem tributaries and headwater tributaries, respectively). I found that local species richness was higher in mainstem tributaries than headwater tributaries and that these effects could not be explained by variation in local environmental habitat conditions. In chapter 2, I developed and applied a continuous model of stream network topology to explore the effects of downstream size and proximity on local fish assemblage structure within the mid-Atlantic highlands. I found that fish assemblage structure (i.e., Bray-Curtis distances in species abundance) was significantly related to variation in stream network topology up to approximately 9 fluvial km from sites. Chapters 3 and 4 explored the implications of inter-stream dispersal for fish bioassessments. In Chapter 3, I identified 10 fish metrics that corresponded predictably to environmental stressors in the mid-Atlantic highlands. However, headwater tributary assemblages showed stronger relations to local environmental quality than mainstem tributaries, consistent with the hypothesis of riverine dispersal. In Chapter 4, I compared the effects of stream network topology on fish and benthic macroinvertebrate assemblages. Fish metrics were influenced by the size and proximity of connected streams but benthic macroinvertebrate metrics were not. This finding suggests that stream fishes may complement benthic macroinvertebrate bioassessments by indicating environmental conditions at larger spatial grains.
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    Environmental Factors Determining the Pre-Restoration Benthic Macroinvertebrate Assemblage In A Stream Used By Cattle
    Willey, Katherine Tara (Virginia Tech, 2008-09-12)
    I investigated the baseline benthic macroinvertebrate community in relation to the environmental conditions in a section of Smith Creek, north of Harrisonburg, VA, prior to restoration. Quantitative benthic macroinvertebrate and environmental samples were collected in April and September 2006 from the Bruce Farm (BR) section of Smith Creek and the nearby Mixed Use (MU) section of Mountain Run. BR had been heavily used for cattle grazing for decades and suffered from sediment, nutrients, and lack of a forested riparian zone. MU had a forested riparian zone, but still received nutrient and sediment inputs from upstream cattle grazing. Visual habitat assessments were performed in September 2006 and were compared to quantitative measures. Benthic macroinvertebrate densities and taxa richness were greater at BR (total density for combined seasons = 52,438; taxa richness for both seasons = 84) than MU (total density for combined seasons = 3,982 and taxa richness for both seasons = 63). Biological environmental variables related to nutrients and growth of plants on rocks (ash-free dry mass, chlorophyll a, epilithic biomass) influenced the benthic macroinvertebrate assemblage more than physical environmental variables related to the substrate composition (% fines, % gravel, Trask's sorting coefficient). Visual habitat estimates were not as effective as quantitative measures of habitat for explaining the benthic macroinvertebrate assemblage.