Population genetics of Michaux's sumac, smooth sumac, and their hybrids

Abstract

Michaux's sumac (Rhus michauxii) is a federally endangered rhizomatous shrub endemic to the southeastern United States, with two of the largest populations located at Maneuver Training Center--Fort Pickett, VA (Fort Pickett), and a nearby property, Deepwater. Michaux's sumac requires soil disturbance and fire to maintain healthy populations. Before being added to the endangered species list, 47% of populations were extirpated due to habitat loss, fire suppression, and hybridization with smooth sumac (Rhus glabra). Concerns with hybridization include hybrid swamping if hybrids are fertile, or outbreeding depression if hybrids display reduced fitness. I used genotyping-by-sequencing to estimate the extent of hybridization at Fort Pickett and Deepwater, and to assess how such hybridization may impact survival of Michaux's sumac as a distinct species at each of these locations. Additionally, population structure was examined using DAPC (discriminant analysis of principal components) and Admixture analyses to determine whether the colonies at Fort Pickett and Deepwater make up separate populations, meta-populations, or one large population. Analysis of 107,344 SNPs (single nucleotide polymorphisms) using Introgress and Admixture software suggested widespread hybridization at both Fort Pickett and Deepwater, with hybrids present in most of the sampled colonies. Population structure analyses revealed differentiation between the Fort Pickett and Deepwater populations, but little evidence of separate populations among the colonies sampled at Fort Pickett. These results are important for conservation planning to ensure the long-term survival of Michaux's sumac at Fort Pickett and Deepwater and can be used to help inform future management decisions.