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dc.contributor.authorAbler, Steven W.en_US
dc.date.accessioned2014-03-14T20:32:57Z
dc.date.available2014-03-14T20:32:57Z
dc.date.issued2003-01-31en_US
dc.identifier.otheretd-03282003-123427en_US
dc.identifier.urihttp://hdl.handle.net/10919/31568
dc.description.abstractLeptosphaerulina spp. are common fungi that have been reported to colonize several turfgrass species. Controversy exists regarding the relationship of Leptosphaerulina spp. and their turfgrass hosts. The fungus has been classified as a saprophyte, senectophyte, weak pathogen, and pathogen of turfgrasses. There has also been conflicting reports regarding the delineation of species within the genus Leptosphaerulina. Because of the uncertainty regarding the ecology and taxonomy of the genus in relation to turfgrasses the present study was undertaken. The ITS and EF-1á gene regions were sequenced and analyzed to compare to the multiple taxonomic schemes reported in the literature. The ITS region offered no resolution of species; however, the phylogeny of the EF-1á gene was consistent with the six-species model of Graham and Luttrell. Inoculation experiments were performed on unstressed and artificially stressed plants to determine whether the fungi are pathogens, senectophytes, or saprophytes of turfgrasses. Perennial ryegrass and creeping bentgrass plants were stressed by placing them in a dew chamber set at 38ºC, 100% R.H., and no light for two and one days respectively. Plants were inoculated with cultures of Leptosphaerulina isolated from turfgrasses, and maintained at optimum conditions reported for infection and colonization. There was no visible difference between inoculated and uninoculated plants, and examination of cleared and stained leaves with a light microscope revealed spores that germinated and produced appressoria, but failed to penetrate the epidermal cells. The lack of infection and colonization suggests that Leptosphaerulina spp. are saprophytes of turfgrasses.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartSteveAbler-Thesis.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectITSen_US
dc.subjectLeptosphaerulinaen_US
dc.subjectEF-1áen_US
dc.subjectphylogeneticsen_US
dc.subjectmorphologyen_US
dc.subjectturfgrassen_US
dc.titleEcology and Taxonomy of Leptosphaerulina spp. Associated with Turfgrasses in the United Statesen_US
dc.typeThesisen_US
dc.contributor.departmentPlant Pathology, Physiology, and Weed Scienceen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplinePlant Pathology, Physiology, and Weed Scienceen_US
dc.contributor.committeechairCouch, H. B.en_US
dc.contributor.committeememberErvin, Erik H.en_US
dc.contributor.committeememberBaudoin, Antonius B. A. M.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-03282003-123427/en_US
dc.date.sdate2003-03-28en_US
dc.date.rdate2004-04-02
dc.date.adate2003-04-02en_US


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